Embryonic stem cells can be used to construct hybrid cell lines containing a single, selectable murine chromosome

Mammalian Genome

Volumn 10, Issue 4, 1999, Pages 381-384

  Jakobs, Petra M ^a   Smith, Lesley ^a   Thayer, Mathew ^a   Grompe, Markus ^a  

^a OREGON HEALTH AND SCIENCE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; AUTOSOME; CHROMOSOME TRANSLOCATION; EMBRYO DEVELOPMENT; GENE DELETION; GENE DISRUPTION; GENE FUNCTION; GENE MAPPING; GENE TARGETING; GENETIC COMPLEMENTATION; HYBRIDIZATION; NONHUMAN; STEM CELL;

ANIMALS; CELL LINE; CHROMOSOMES; CRICETINAE; GENETIC MARKERS; GENETIC TECHNIQUES; HUMANS; HYBRID CELLS; MICE; RATS; RECOMBINATION, GENETIC; STEM CELLS;

EID: 0032776756     PISSN: 09388990     EISSN: None     Source Type: Journal    
DOI: 10.1007/s003359901006     Document Type: Article

References (19)

1. Athwal RS, Smarsh M, Searle BM, Deo SS (1985) Integration of a dominant selectable marker into human chromosomes and transfer of marked chromosomes to mouse cells by microcell fusion. Somatic Cell Mol Genet 11, 177-187
2. Cuthbert AP, Trott DA, Ekong RM, Jezzard S, England NL, et al. (1995) Construction and characterization of a highly stable human: rodent monochromosomal hybrid panel for genetic complementation and genome mapping studies. Cytogenet Cell Genet 71, 68-76
3. Davis AC, Wims M, Spotts GD, Hann SR, Bradley A (1993) A null c-myc mutation causes lethality before 10.5 days of gestation in homozygotes and reduced fertility in heterozygous female mice. Genes Dev 7, 671-682
4. Engle SJ, Stockelman MG, Chen J, Bolvin G, Yum MN et al. Adenine phosphoribosyltransferase-deficient mice develop 2,8-dihydroxyadenine nephrolithiasis. Proc Natl Acad Sci USA 93, 5307-5312
5. Fournier RE (1981) A general high-efficiency procedure for production of microcell hybrids. Proc Natl Acad Sci USA 78, 6349-6353
6. Fournier RE, Frelinger JA (1982) Construction of microcell hybrid clones containing specific mouse chromosomes: application to autosomes 8 and 17. Mol Cell Biol 2, 526-534
7. Gosden PD (1987) HRASI-selected, chromosome-mediated gene transfer; in situ hybridization with combined biiotin and tritium label localizes the oncogene and reveals duplications of the human transgenome. Cytogenet Cell Genet 45, 44-51
8. Islam K, Islam MQ (1994) Assignment of TK1 encoding thymidine kinase to Syrian hamster chromosome 9 by microcell-mediated chromosome transfer. Cytogenet Cell Genet 66, 177-180
9. Klein KG, Bouck NP (1994) The distal region of the long arm of human chromosome 1 carries tumor suppressor activity for a human fibrosarcoma line. Cancer Genet Cytogenet 73, 109-121
10. Lugo TG, Handelin B, Killary AM, Housman DE, Fournier RE (1987) Isolation of microcell hybrid clones containing retroviral vector insertions into specific human chromosomes. Mol Cell Biol 7, 2814-2820
11. McNeill CA, Brown RL (1980) Genetic manipulation by means of microcell-mediated transfer of normal human chromosomes into recipient mouse cells. Proc Natl Acad Sci USA 77, 5394-5398
12. Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16, 1215
13. Satoh H, Barrett JC, Oshimura M (1991) Introduction of new genetic markers on human chromosomes. Exp Cell Res 193, 5-11
14. Saxon PJ, Srivatsan ES, Stanbridge EJ (1986) Introduction of human chromosome 11 via microcell transfer controls tumorigenic expression of HeLa cells. Embo J 5, 3461-3466
15. Schultz RA, Saxon PJ, Glover TW, Friedberg EC (1987) Microcell-mediated transfer of a single human chromosome complements xeroderma pigmentosum group A fibroblasts. Proc Natl Acad Sci USA 84, 4176-4179
16. Seabright M (1971) A rapid banding technique for human chromosomes. Lancet 2, 971-972
17. Sid'en TS, Hoglund M, Rohme D (1989) Construction of microcell hybrid panel containing different neo gene insertions in mouse chromosome 17 used for chromosome-mediated gene transfer. Somatic Cell Mol Genet 15, 245-253
18. Stein PL, Lee HM, Rich S, Soriano P (1992) pp59fyn mutant mice display differential signaling in thymocytes and peripheral T cells. Cell 70, 741-750
19. Warburton D, Gersen S, Yu MT, Jackson C, Handelin B et al. (1990) Monochromosomal rodent-human hybrids from microcell fusion of human lymphoblastoid cells containing an inserted dominant selectable marker. Genomics 6, 358-366