Simultaneous oligonucleotide probe hybridization and immunostaining for in situ detection of Gordona species in activated sludge

FEMS Microbiology Ecology

Volumn 29, Issue 2, 1999, Pages 129-136

  Oerther, Daniel B ^a   De Los Reyes Iii, Francis L ^a   Hernandez, Mark ^b   Raskin, Lutgarde ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b UNIVERSITY OF COLORADO   (United States)

Author keywords

Activated sludge; Antibody stain; Gordona; Oligonucleotide probe; rRNA

Indexed keywords

POLYCLONAL ANTIBODY; RIBOSOME RNA;

ACTIVATED SLUDGE; METHODOLOGY; MICROBIAL COMMUNITY;

ACTIVATED SLUDGE; ARTICLE; CONTROLLED STUDY; FUNGUS GROWTH; GORDONA; GORDONIA AMARAE; IMMUNOHISTOCHEMISTRY; NONHUMAN; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; RNA HYBRIDIZATION;

FUNGI; GORDONIA (ACTINOMYCETE); GORDONIA AMARAE; NOCARDIA;

EID: 0032776672     PISSN: 01686496     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-6496(99)00005-7     Document Type: Article

References (16)

1. Soddell, J.A. and Seviour, R.J. (1990) A review: microbiology of foaming in activated sludge plants. J. Appl. Bacteriol. 69, 145-176.
2. Jenkins, D., Richard, M.G. and Daigger, G.T. (1993) Manual on the Causes and Control of Activated Sludge Bulking and Foaming, 2nd edn., 193 pp. Lewis, Chelsea.
3. Wanner, J. (1994) Activated Sludge Bulking and Foaming Control, 1st edn., 327 pp. Technomic, Pennsylvania.
4. Lechevalier, M.P. and Lechevalier, H.A. (1974) Nocardia amarae sp. nov., an actinomycete common in foaming activated sludge. Int. J. System. Bacteriol. 24, 278-288.
5. Klatte, S., Rainey, F. and Kroppenstedt, R.M. (1994) Transfer of Rhodococcus aichiensis tsukamura 1982 and Nocardia amarae lachevalier 1974 to the genus Gordona as Gordona aichiensis comb. nov. and Gordona amarae comb. nov. Int. J. Syst. Bacteriol. 44, 769-773.
6. Amann, R., Lemmer, H. and Wagner, M. (1998) Monitoring the community structure of wastewater treatment plants: a comparison of old and new techniques. FEMS Microbiol. Ecol. 25, 205-215.
7. Stahl, D.A. (1997) Molecular approaches for the measurement of density, diversity, and phylogeny. In: Manual of Environmental Microbiology (Hurst, C.J., Knudsen, G.R., McInerney, M.J., Stetzenbach, L.D. and Walter, M.V., Eds.), pp. 102-114. American Society for Microbiology, Washington, DC.
8. Li, X., de Boer, S.H. and Ward, L.J. (1997) Improved microscopic identification of Clavibacter michiganesis subsp. sepedonicus cells by combining in situ hybridization with immunofluorescence. Lett. Appl. Microbiol. 24, 431-434.
9. Abmus, B., Schloter, M., Kirchhof, G., Hutzler, P. and Hartmann, A. (1997) Improved in situ tracking of rhizosphere bacteria using dual staining with fluorescence-labeled antibodies and rRNA-targeted oligonucleotides. Microb. Ecol. 33, 32-40.
10. Hernandez, M., Jenkins, D. and Beaman, B.L. (1994) Mass and viability estimations of Nocardia in activated sludge and anaerobic digestors using conventional stains and immunofluorescent methods. Water Sci. Technol. 29, 249-259.
11. de los Reyes, F.L., Ritter, W. and Raskin, L. (1997) Group-specific small subunit rRNA hybridization probes to characterize filamentous foaming in activated sludge systems. Appl. Environ. Microbiol. 63, 1107-1117.
12. de los Reyes, F.L., Oerther, D.B., de los Reyes, M.F., Hernandez, M. and Raskin, L. (1998) Characterization of filamentous foaming in activated sludge systems using oligonucleotide hybridization probes and antibody probes. Water Sci. Technol. 37 (4-5), 485-493.
13. Hernandez, M. and Jenkins, D. (1994) The fate of Nocardia in anaerobic digestion. Water Environ. Res. 66, 828-835.
14. Bohlool, B.B. and Schmidt, E.L. (1980) The immunofluorescence approach in microbial ecology. Adv. Microb. Ecol. 4, 203-241.
15. DeLong, E.F., Wickham, G.S. and Pace, N.R. (1989) Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells. Science 243, 1360-1363.
16. Poulsen, L.K., Ballard, G. and Stahl, D.A. (1993) Use of rRNA fluorescent in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl. Environ. Microbiol. 59, 1354-1360.