Flow cytometry and RT-PCR for rotavirus detection in artificially seeded oyster meat

International Journal of Food Microbiology

Volumn 49, Issue 1-2, 1999, Pages 9-18

  Barardi, Celia R M ^a,b   Yip, Hopi ^c   Emsile, Kerry R ^b   Vesey, Graham ^b   Raj Shanker, S ^c   Williams, Keith L ^b  

^a Univ Fed S C   (Brazil)

^b MACQUARIE UNIVERSITY   (Australia)

^c Water Technology Australia   (Australia)

Author keywords

Flow cytometry; Oysters; Rotavirus; RT PCR

Indexed keywords

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FLOW CYTOMETRY; NONHUMAN; OYSTER; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ROTAVIRUS; VIRUS DETECTION;

ANIMALS; FLOW CYTOMETRY; MEAT; MICE; OSTREIDAE; POLYMERASE CHAIN REACTION; ROTAVIRUS; SENSITIVITY AND SPECIFICITY;

EID: 0032776601     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1605(99)00040-9     Document Type: Article

References (26)

1. Abad F.X., Bosch A., Comas J., Villalba D., Pintó R.M. Evaluation of flow cytometry for the monitoring of infectious human rotavirus in water. Water Sci. Tech. 35:1997;451-453.
2. Barardi C.R.M., Emslie K.R., Vesey G., Williams K.L. Development of a rapid and sensitive quantitative assay for rotavirus based on flow cytometry. J. Virol. Meth. 74:1998;31-38.
3. Bej A.K., Mahbubani M.H. Applications of the polymerase chain reaction (PCR) in vitro DNA-amplification method in environmental microbiology. Griffin H.G., Griffin A.M. PCR Technology: Current Innovations. 1994;327-339 CRC Press, Boca Raton, FL.
4. Bouchriti N., Goyal S.M. Methods for the concentration and detection of human enteric viruses in shellfish: a review. Microbiologica. 16:1993;105-114.
5. Chen D., Ramig R.F. Determinants of rotavirus stability during CsCl purification. Virology. 186:1992;228-237.
6. Cromeans T.L., Nainan O.V., Margolis H.S. Detection of hepatitis A virus RNA in oyster meat. Appl. Environ. Microbiol. 63:1997;2460-2463.
7. Deetz T.R., Smith E.M., Goyal S.M., Gerba C.P., Vollet J.J. III, Tsai L., Pont H.L., Keswick B.H. Occurrence of rota and enteroviruses in developing nation. Water Res. 18:1984;567-571.
8. Estes M.K., Palmer E.L., Objeski J.F. Rotaviruses: a review. Curr. Top. Microbiol. Immunol. 105:1983;123-184.
9. Gouvea V., Glass R.I., Woods P., Taniguchi K., Clark H.F., Forrester B., Fang Z.Y. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J. Clin. Microbiol. 28:1990;276-282.
10. Goyal S.M., Gerba C.P. Viradel method for detection of rotavirus in seawater. J. Virol. Methods. 7:1983;95-106.
11. Grinde B., Jonassen T.Ø., Ushijima H. Sensitive detection of group A rotaviruses by immunomagnetic separation and reverse transcription-polymerase chain reaction. J. Virol. Methods. 55:1995;327-338.
12. Hafliger D., Gilger M., Luthy J., Hubner P. Seminested RT-PCR systems for small round structured viruses and detection of enteric viruses in seafood. Int. J. Food Microbiol. 37:1997;27-36.
13. Hoffman R.M., Standridge J.H., Cucunato J.C., Bernhardt M. Using flow cytometry to detect protozoa. J. Am. Water Works Assoc. 89:1997;104-111.
14. Imbert-Marcille B.M., Robillard N., Poirier A.S., Coste-Burel M., Cantarovich D., Milpied N., Billaudel S. Development of a method for direct quantitation of cytomegalovirus antigenemia by flow cytometry. J. Clin. Microbiol. 35:1997;2665-2669.
15. Kostenbader K.D., Cliver D. Polyelectrolyte flocculation as an aid to recovery of enteroviruses from oysters. Appl. Microbiol. 24:1972;540-543.
16. Lewis G.D., Metcalf T.G. Polyethylene Glycol precipitation for recovery of pathogenic viruses, including rotavirus, from oyster, water and sediment samples. Appl. Environ. Microbiol. 54:1988;1983-1988.
17. Limsawat S., Ohgaki S. Fate of liberated viral RNA in wastewater determined by PCR. Appl. Environ. Microbiol. 63:1997;2932-2933.
18. Schwabb K.J., deLeon R., Sobsey M.D. Concentration and purification of beef extracts mock eluates from water samples for the detection of enteroviruses, hepatitis A virus, and Norwalk virus by reverse-transcription-PCR. Appl. Environ. Microbiol. 61:1995;531-537.
19. Scillian J.J., McHugh T.M., Busch M.P., Tam M., Fulwyler M.J., Chien D.Y., Vyas G.N. Early detection of antibodies against rDNA-produced HIV proteins with a flow cytometric assay. Blood. 73:1989;2041-2048.
20. Shaw R.D., Vo P.T., Offit P.A., Coulson B.S., Greenberg H.B. Antigenic mapping of the surface proteins of rhesus rotavirus. Virology. 155:1986;434-451.
21. Smith E.M., Gerba C.P. Development of a method for detection of human rotavirus in water and sewage. Appl. Environ. Microbiol. 43:1982;1440-1450.
22. Strappe P. Rotavirus detection - a problem that needs concentration. Water Sci. Tech. 24:1991;221-223.
23. Vaneechoutte M., Van Eldere J. The possibilities and limitations of nucleic acid amplification technology in diagnostic microbiology. J. Med. Microbiol. 46:1997;188-194.
24. Vesey G., Narai J., Ashbolt N., Williams K.L., Veal D. Detection of specific microorganisms in environmental samples using flow cytometry. Methods Cell Biol. 42:1994;489-522 Academic Press, New York. Ch. 29.
25. Wallner G., Fuchs B., Spring S., Beisker W., Amann R. Flow sorting of microorganisms for molecular analysis. Appl. Environ. Microbiol. 63:1997;4223-4231.
26. Wilson I.G. Inhibition and facilitation of nucleic acid amplification - minireview. Appl. Environ. Microbiol. 63:1997;3741-3751.