Leukocyte counting with the Nageotte hemocytometer: Use of fluorescence microscopy

Infusionstherapie und Transfusionsmedizin

Volumn 26, Issue 4, 1999, Pages 222-225

  Graldi, G ^a   Matteucci, G ^a   Reverberi, R ^a,b  

^a UNIVERSITY HOSPITAL OF FERRARA   (Italy)

^b Serv Immunoematologia Trasfusionale   (Italy)

Author keywords

Fluorescence microscopy; Leukocyte counts; Nageotte hemocytometer

Indexed keywords

FLUOROCHROME; HEMOGLOBIN;

ARTICLE; CYTOMETRY; FLUORESCENCE MICROSCOPE; HUMAN; HUMAN CELL; LEUKOCYTE COUNT;

EID: 0032770397     PISSN: 10198466     EISSN: None     Source Type: Journal    
DOI: 10.1159/000053492     Document Type: Article

References (7)

1. Masse M, Naegelen C, Pellegrini N, Segier JM, Marpaux N, Beujean F: Validation of a simple method to count very low white cell concentrations in filtered red cells or platelets. Transfusion 1992; 32:565-571.
2. Lutz P, Dzik WH: Large-volume hemocytometer chamber for accurate counting of white cells (WBCs) in WBC-reduced platelets: Validation and application for quality control of WBC-reduced platelets prepared by apheresis and filtration. Transfusion 1993;33:409-412.
3. Moroff G, Eich J, Dabay M: Validation of use of the Nageotte hemocytometer to count low levels of white cells in white-cell reduced platelet components. Transfusion 1994;34:35-38.
4. Rebulla P, Dzik WH: Multicenter evaluation of methods for counting residual white cells in leukocyte-depleted red blood cells. Vox Sang 1994;66: 25-32.
5. Nishimura T, Oka S, Yamawaki N: Technical aspects of leukocyte depletion; in Högman CF (ed): Leukocyte Depletion of Blood Components. Amsterdam, VU University Press, 1994, pp 51-58.
6. Dzik WH, Szuflad P: Method for counting white cells (WBCs) in WBC-reduced red cell concentrates. Transfusion 1993;33:272-273.
7. Dumont LJ: Sampling errors and the precision associated with counting very low numbers of white cells in blood components. Transfusion 1991;31: 428-432.