Protein salvage by directed evolution. Functional restoration of a defective lysozyme mutant

Annals of the New York Academy of Sciences

Volumn 870, Issue , 1999, Pages 404-407

  Jucovic, Milan ^a   Poteete, Anthony R ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LYSOZYME; MUTANT PROTEIN;

CONFERENCE PAPER; DNA RECOMBINATION; ENZYME INACTIVATION; EVOLUTION; MUTAGENESIS;

EID: 0032768957     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1111/j.1749-6632.1999.tb08914.x     Document Type: Conference Paper

References (7)

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2. RENNELL, D., S.E. BOUVIER, L.W. HARDY & A.R. POTEETE. 1991. Systematic mutation of bacteriophage T4 lysozyme. J. Mol. Biol. 222: 67-88.
3. POTEETE, A.R., D. RENNELL & S.E. BOUVIER 1992. Functional significance of conserved amino acid residues. Proteins 13: 38-40.
4. BOUVIER, S.E. & A.R. POTEETE. 1996. Second-site reversion of a structural defect in bacteriophage T4 Iysozyme. FASEB J. 10: 159-163.
5. POTEETE, A.R., D. RENNELL, S.E. BOUVIER & L.W. HARDY. 1997. Alteration of T4 Iysozyme structure by second-site reversion of deleterious mutations. Prot. Sci. 6: 2418-2425.
6. STEMMER, W.P.C. 1994. Rapid evolution of a protein in vitro by DNA shuffling. Nature 370: 389-391.
7. SAYLE, R.A. & E.J. MILNER-WHITE. 1995. RASMOL: Biomolecular graphics for all. Trends Biochem. Sci. 20:374-376.