Detection of intrastrain antigenic variation of Bacteroides fragilis surface polysaccharides by monoclonal antibody labelling

Infection and Immunity

Volumn 67, Issue 9, 1999, Pages 4346-4351

  Patrick, Sheila ^a   Gilpin, Deirdre ^a   Stevenson, Leanne ^a  

^a QUEEN'S UNIVERSITY BELFAST   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL POLYSACCHARIDE; EPITOPE; MONOCLONAL ANTIBODY;

ANTIBODY SPECIFICITY; ANTIGEN EXPRESSION; ARTICLE; BACTERIAL INFECTION; BACTERIUM CULTURE; BACTERIUM ISOLATION; BACTEROIDES FRAGILIS; DIAGNOSTIC TEST; GRAM NEGATIVE INFECTION; IMMUNOFLUORESCENCE MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; STRAIN DIFFERENCE;

EID: 0032768793     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.67.9.4346-4351.1999     Document Type: Article

References (28)

1. Cruickshank, R. 1965. Medical microbiology: a guide to the laboratory diagnosis and control of infection, 11th ed., p. 657-660. Churchill Livingstone, Edinburgh, United Kingdom.
2. Davies, D. G., M. R. Parsek, J. P. Pearson, B. H. Iglewski, J. W. Costerton, and E. P. Greenberg. 1998. The involvement of cell-to-cell signals in the development of a bacterial biofilm. Science 280:295-298.
3. Delahooke, D. M., G. R. Barclay, and I. R. Poxton. 1995. A re-appraisal of the biological activity of bacteroides LPS. J. Med. Microbiol. 42:102-112.
4. Duerden, B. I., and B. S. Drasar (ed.). 1991. Anaerobes in human disease. Edward Arnold, London, United Kingdom.
5. Lutton, D. A., S. Patrick, A. D. Crockard, L. D. Stewart, M. J. Larkin, E. Dermott, and T. A. McNeill. 1991. Flow cytometric analysis of within-strain variation in polysaccharide expression by Bacteroides fragilis by use of murine monoclonal antibodies. J. Med. Microbiol. 35:229-237.
6. Namavar, F., E. B. Theunissen, A. M. J. J. Verweij-Van Vught, P. G. Peerbooms, M. Bal, H. F. Hoitsma, and D. M. MacLaren. 1989. Epidemiology of the Bacteroides fragilis group in the colonic flora of patients with colonic cancer. J. Med. Microbiol. 19:171-176.
7. Pantosti, A., R. Colangeli, A. O. Tzianabos, and D. L. Kasper. 1995. Monoclonal antibodies to detect capsular diversity among Bacieroides fragilis isolates. J. Clin. Microbiol. 33:2647-2652.
8. Pantosti, A., A. O. Tzianabos, A. B. Onderdonk, and D. L. Kasper. 1991. Immunochemical characterization of two surface polysaccharides of Bacteroides fragilis. Infect. Immun. 59:2075-2082.
9. Patrick, S. 1993. The virulence of Bacteroides fragilis. Rev. Med. Microbiol. 4:40-49.
10. Patrick, S. 1997. Variation of Bacteroides fragilis surface structures, p. 19-30. In A. R. Eley and K. W. Bennett (ed.), Anaerobic pathogens. Sheffield Academic Press, Sheffield, United Kingdom.
11. Patrick, S., and M. J. Larkin. 1993. Attachment in disease, p. 109-131. In S. P. Denyer, S. P. Gorman, and M. Sussman (ed.), Society for Applied Bacteriology Technical Series, 30. Microbial biofilms: formation and control. Blackwell Scientific Publications, Oxford, United Kingdom.
12. Patrick, S., and M. J. Larkin. 1995. Immunological and molecular aspects of bacterial virulence. John Wiley & Sons, Chichester, United Kingdom.
13. Patrick, S., and D. A. Lutton. 1990. Bacteroides fragilis surface structure expression in relation to virulence. Med. Mal. Infect. 20:19-25.
14. Patrick, S., J. P. McKenna, S. O'Hagan, and E. Dermott. 1996. A comparison of the haemagglutinating and enzymic activities of Bacteroides fragilis whole cells and outer membrane vesicles. Microb. Pathog. 20:191-202.
15. Patrick, S., and J. H. Reid. 1983. Separation of capsulate and non-capsulate Bacteroides fragilis on a discontinuous density gradient. J. Med. Microbiol. 16:239-241.
16. Patrick, S., J. H. Reid, and A. Colfey. 1986. Capsulation of in vitro and in vivo grown Bacteroides species. J. Gen. Microbiol. 132:1099-1109.
17. Patrick, S., L. D. Stewart, N. Damani, K, G. Wilson, D. A. Lutton, M. J. Larkin, I. Poxton, and R. Brown. 1995. Immunological detection of Bacteroides fragilis in clinical samples. J. Med. Microbiol. 43:99-109.
18. Poxton, I. R., and R. Brown. 1986. Immunochemistry of the surface carbohydrate antigens of Bacteroides fragilis and definition of a common antigen. J. Gen. Microbiol. 132:2475-2481.
19. Poxton, I. R., R. Brown, A. Sawyerr, and A. Ferguson. 1997. Mucosa-associated bacterial flora of the human colon. J. Med. Microbiol. 46:85-91.
20. Ramage, G., S. Patrick, and S. Houstoń. 1998. Combined fluorescent in situ hybridisation and immunolabelling of Bacteroides fragilis. J. Immunol. Methods 212:139-147.
21. Reid, J. H., and S. Patrick. 1984. Phagocytic and serum killing of capsulate and non-capsulate Bacteroides fragilis. J. Med. Microbiol. 17:247-257.
22. Reid, J. H., S. Patrick, E. Dermott, A. Trudgett, and S. Tabaqchali. 1985. Investigation of antigenic expression of Bacteroides fragilis by immunogold labelling and immunoblotting with a monoclonal antibody. FEMS Microbiol. Lett. 30:289-293.
23. Reid, J. H., S. Patrick, and S. Tabaqchali. 1987. Imrnunochemical characterization of a polysaccharide antigen of Bacteroides fragilis with an IgM-monoclonal antibody. J. Gen. Microbiol. 133:171-179.
24. Seifert, H., and M. So. 1988. Genetic mechanisms of bacterial antigenic variation. Microbiol. Rev. 52:327-336.
25. Stewart, L. D. 1993. Ph.D. thesis. Queen's University of Belfast, Belfast, United Kingdom.
26. Stewart, L. D., S. Patrick, D. A. Lutton, M. J. Larkin, I. R. Poxton, and R. Brown. 1992. Immunological detection of polysaccharide antigens of Bacteroides fragilis in human pus samples, p. 55-56. In B. I. Duerden, J. S. Brazier, S. V. Seddon, and W. G. Wade (ed.), Medical and environmental aspects of anaerobes. Wrightson Biomedical Publishing Ltd., Petersfield, United Kingdom.
27. Tzianabos, A. O., A. B. Onderdonk, B. Rosner, R. L. Cisneros, and D. L. Kasper. 1993. Structural features of polysaccharides that induce intra-abdominal abscesses. Science 262:416-419.
28. van Tassell, R. L., and T. D. Wilkins. 1978. Isolation of auxotrophs of Bacteroides fragilis. Can. J. Microbiol. 24:1619-1621.