Overcoming false negatives due to the genomic context in polymerase chain reaction amplification

Journal of Biochemical and Biophysical Methods

Volumn 40, Issue 1-2, 1999, Pages 45-48

  Baldrich, E ^a   Xamena, N ^a   Cabre O ^a  

^a Dept Genet I M ^*  (Spain)

Author keywords

Drosophila melanogaster; Mutation; PCR specificity; White locus

Indexed keywords

RESTRICTION ENDONUCLEASE;

AGAR GEL ELECTROPHORESIS; ANIMAL CELL; DROSOPHILA MELANOGASTER; GENE AMPLIFICATION; GENOME; MUTANT; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT; SHORT SURVEY;

DROSOPHILA MELANOGASTER; MELANOGASTER;

EID: 0032767187     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(99)00017-2     Document Type: Article

References (6)

1. Sharma J.K., Gopalkrishna V., Das B.C. A simple method for elimination of unspecific amplifications in polymerase chain reaction. Nucleic Acids Res. 20:1992;6117-6118.
2. Hatchwell E. Improving PCR specificity by multiple pre-digestion. Trends Genet. 12:1996;460.
3. a allele at the white locus of D. melanogaster. Cell. 5:1981;705-711.
4. Levis R., Bingham P., Rubin G. Physical map of the white locus of Drosophila melanogaster. Proc. Natl. Acad. Sci. USA. 79:1982;564-568.
5. O'Hare K., Murphy C., Levis R., Rubin G.M. DNA sequence of the white locus of Drosophila melanogaster. J. Mol. Biol. 80:1984;437-455.
6. O'Hare K. EMBL/GenBank accession number X02974, 1986.