The cysteine-rich region of the Entamoeba histolytica adherence lectin (170-kilodalton subunit) is sufficient for high-affinity Gal/GalNac-specific binding in vitro

Infection and Immunity

Volumn 67, Issue 8, 1999, Pages 3836-3841

  Pillai, Dylan R ^a   Wan, Peter S K ^a   Yau, Yvonne C W ^a   Ravdin, Jonathan I ^b   Kain, Kevin C ^c  

^a UNIVERSITY HEALTH NETWORK   (Canada)

^b UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^c TORONTO GENERAL HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

ASIALOFETUIN; LUCIFERASE; PROTEIN DISULFIDE ISOMERASE;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL ADHESION; CHO CELL; ENTAMOEBA HISTOLYTICA; ENZYME ACTIVITY; IMMUNOREACTIVITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING;

ENTAMOEBA HISTOLYTICA;

EID: 0032765411     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.67.8.3836-3841.1999     Document Type: Article

References (34)

1. Adler, P., S. J. Wood, Y. C. Lee, R. T. Lee, W. A. Petri, Jr., and R. L. Schnaar. 1995. High affinity binding of the Entamoeba histolytica lectin to polyvalent N-acetylgalactosaminides. J. Biol. Chem. 270:5164-5171.
2. Aley, S. B., W. A. Scott, and Z. A. Cohn. 1980. Plasma membrane of Entamoeba histolytica. J. Exp. Med. 152:391-404.
3. Campbell, D., and K. Chadee. 1997. Survival strategies of Entamoeba histolytica: modulation of cell-mediated immune responses. Parasitol. Today 13:184-189.
4. Chadee, K., M. L. Johnson, E. Orozco, W. A. Petri, Jr., and J. I. Ravdin. 1988. Binding and internalization of rat colonic mucins by the galactose/N-acetyl-D-galactosamine adherence lectin of Entamoeba histolytica. J. Infect. Dis. 158:398-406.
5. Chadee, K., W. A. Petri, Jr., D. J. Innes, and J. I. Ravdin. 1987. Rat and human colonic mucins bind to and inhibit adherence lectin of Entamoeba histolytica. J. Clin. Investig. 80:1245-1254.
6. Diamond, L. S., D. R. Harlow, and C. C. Cunnick. 1978. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans. R. Soc. Trop. Med. Hyg. 72:431-432.
7. Gilbert, H. F. 1997. Protein disulfide isomerase and assisted protein folding. J. Biol. Chem. 272:29399-29402.
8. Guerrant, R. L., J. Brush, J. I. Ravdin, J. A. Sullivan, and G. L. Mandell. 1981. Interaction between Entamoeba histolytica and human polymorphonuclear neutrophils. J. Infect. Dis. 143:83-93.
9. Leippe, M., J. Andra, and H. J. Muller-Eberhard. 1994. Cytolytic and antibacterial activity of synthetic peptides derived from amoebapore, the pore-forming peptide of Entamoeba histolytica. Proc. Natl. Acad. Sci. USA 91: 2602-2606.
10. Lotter, H., T. Zhang, K. B. Seydel, S. L. Stanley, Jr., and E. Tannich. 1997 Identification of an epitope on the Entamoeba histolytica 170-kD lectin conferring antibody-mediated protection against invasive amebiasis. J. Exp. Med. 185:1793-1801.
11. Mann, B. J., C. Y. Chung, J. M. Dodson, L. S. Ashley, L. L. Braga, and T. L. Snodgrass. 1993. Neutralizing monoclonal antibody epitopes of the Entamoeba histolytica galactose adhesin map to the cysteine-rich extracellular domain of the 170-kilodalton subunit. Infect. Immun. 61:1772-1778.
12. Mann, B. J., and L. A. Lockhart. 1998. Molecular analysis of the Gal/ GalNAc adhesin of Entamoeba histolytica. J. Eukaryot. Microbiol. 45:13S-16S.
13. Mann, B. J., B. E. Torian, T. S. Vedvick, and W. A. Petri, Jr. 1991. Sequence of a cysteine-rich galactose-specific lectin of Entamoeba histolytica. Proc. Natl. Acad. Sci. USA 88:3248-3252.
14. McCoy, J. J., B. J. Mann, T. S. Vedvick, Y. Pak, D. B. Heimark, and W. A. Petri, Jr. 1993. Structural analysis of the light subunit of the Entamoeba histolytica galactose-specific adherence lectin. J. Biol. Chem. 268:24223-24231.
15. McCoy, J. J., B. J. Mann, T. S. Vedvick, and W. A. Petri, Jr. 1993. Sequence analysis of genes encoding the light subunit of the Entamoeba histolytica galactose-specific adhesin. Mol. Biochem. Parasitol. 61:325-328.
16. Petri, W. A., Jr., M. P. Joyce, J. Broman, R. D. Smith, C. F. Murphy, and J. I. Ravdin. 1987. Recognition of the galactose- or N-acetylgalactosamine-binding lectin of Entamoeba histolytica by human immune sera. Infect. Immun. 55:2327-2331.
17. Petri, W. A., Jr., and R. L. Schnaar. 1995. Purification and characterization of galactose- and N-acetylgalactosamine-specific adhesin lectin of Entamoeba histolytica. Methods Enzymol. 253:98-104.
18. Petri, W. A., Jr., R. D. Smith, P. H. Schlesinger, C. F. Murphy, and J. I. Ravdin. 1987. Isolation of the galactose-binding lectin that mediates the in vitro adherence of Entamoeba histolytica. J. Clin. Investig. 80:1238-1244.
19. Petri, W. A., Jr., T. L. Snodgrass, T. F. Jackson, V. Gathiram, A. E. Simjee, K. Chadee, and M. D. Chapman. 1990. Monoclonal antibodies directed against the galactose-binding lectin of Entamoeba histolytica enhance adherence. J. Immunol. 144:4803-4809.
20. Pillai, D. R., D. Britten, J. P. Ackers, J. I. Ravdin, and K. C. Kain. 1997. A gene homologous to hgl2 of Entamoeba histolytica is present and expressed in Entamoeba dispar. Mol. Biochem. Parasitol. 87:101-105.
21. 20a. Promega Corp. 1995. Protocols & applications guide, 2nd ed. Promega Corp., Madison, Wis.
22. Purdy, J. E., B. J. Mann, E. C. Shugart, and W. A. Petri, Jr. 1993. Analysis of the gene family encoding the Entamoeba histolytica galactose-specific adhesin 170-kDa subunit. Mol. Biochem. Parasitol. 62:53-59.
23. Ravdin, J. I., B. Y. Croft, and R. L. Guerrant. 1980. Cytopathogenic mechanisms of Entamoeba histolytica. J. Exp. Med. 152:377-390.
24. Ravdin, J. I., and R. L. Guerrant. 1981. Role of adherence in cytopathogenic mechanisms of Entamoeba histolytica. Study with mammalian tissue culture cells and human erythrocytes. J. Clin. Investig. 68:1305-1313.
25. Ravdin, J. I., and B. L. Kelsall. 1994. Role of mucosal secretory immunity in the development of an amebiasis vaccine. Am. J. Trop. Med. Hyg. 50:36-41.
26. Ravdin, J. I., W. A. Petri, C. F. Murphy, and R. D. Smith. 1986. Production of mouse monoclonal antibodies which inhibit in vitro adherence of Entamoeba histolytica trophozoites. Infect. Immun. 53:1-5.
27. Reed, S. L., J. G. Curd, I. Gigli, F. D. Gillin, and A. I. Braude. 1986. Activation of complement by pathogenic and nonpathogenic Entamoeba histolytica. J. Immunol. 136:2265-2270.
28. Ruddon, R. W., and E. Bedows. 1997. Assisted protein folding. J. Biol. Chem. 272:3125-3128.
29. Saffer, L. D., and W. J. Petri. 1991. Role of the galactose lectin of Entamoeba histolytica in adherence-dependent killing of mammalian cells. Infect. Immun. 59:4681-4683.
30. Tannich, E., F. Ebert, and R. D. Horstmann. 1992. Molecular cloning of cDNA and genomic sequences coding for the 35-kilodalton subunit of the galactose-inhibitable lectin of pathogenic Entamoeba histolytica. Mol. Biochem. Parasitol. 55:225-227.
31. Tannich, E., F. Ebert, and R. D. Horstmann. 1991. Primary structure of the 170-kDa surface lectin of pathogenic Entamoeba histolytica. Proc. Natl. Acad. Sci. USA 88:1849-1853.
32. Tannich, E., H. Scholze, R. Nickel, and R. D. Horstmann. 1991. Homologous cysteine proteinases of pathogenic and nonpathogenic Entamoeba histolytica. Differences in structure and expression. J. Biol. Chem. 266:4798-4803.
33. Walsh, J. A. 1986. Problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality. Rev. Infect. Dis. 8:228-238.
34. Zhang, T., and S. L. Stanley, Jr. 1994. Protection of gerbils from amebic liver abscess by immunization with a recombinant protein derived from the 170-kilodalton surface adhesin of Entamoeba histolytica. Infect. Immun. 62:2605-2608.