Pepstatin-sensitive proteolytic activity of sorghum seeds

Biotechnology Techniques

Volumn 13, Issue 11, 1999, Pages 817-820

  Macedo, I Queiroz ^a   Marques, Paulo ^a   Delgadillo, I ^a  

^a UNIVERSITY OF AVEIRO   (Portugal)

Author keywords

Aspartic proteinase; Pepstatin; Proteinase; Sorghum

Indexed keywords

EDETIC ACID; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME SUBSTRATE; ENZYME SUBUNIT; HEMOGLOBIN; LEUPEPTIN; N [N (3 CARBOXYOXIRANE 2 CARBONYL)LEUCYL]AGMATINE; PEPSTATIN; PH; PROTEIN HYDROLYSIS; PROTEINASE; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS;

SORGHUM;

EID: 0032762964     PISSN: 0951208X     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1008912905483     Document Type: Article

References (12)

1. Asakura T, Watanabe H, Abe K, Arai S (1997) Oryzasin as an aspartic proteinase occurring in rice seeds: purification, characterization and application to milk-clotting. J. Agric. Food Chem. 45: 1070-1075.
2. Dunaevsky YE, Sarbakanova ST, Belozersky MA (1989) Wheat seed carboxypeptidase and joint action on gliadin of proteases from dry and germinating seeds. J. Exp. Bot. 40: 1323-1329.
3. Galleschi L, Fèlicioli F (1994) Purification, characterization and activation by anions of an aspartic proteinase isolated from bran of soft wheat. Plant Sci. 98: 15-24.
4. Kervinen J, Tormakangas K, Runeberg-Roos P, Guruprasad K, Blundell T, Teeri T (1995) Structure and possible function of aspartic proteinases in barley and other plants. In: Takahashi K (ed.) Aspartic Proteinases: Structure, Function, Biology and Biomedical Implications. New York: Plenum Press, pp. 241-254.
5. Macedo IQ, Faro CJ, Pires EV (1993) Specificity and kinetis of the milk-clotting enzyme from cardoon (C. cardunculus L.) toward κ-casein. J. Agric. Food Chem. 41: 1537-1540.
6. Macedo IQ, Faro CJ, Pires EV (1996) Caseinolytic specificity of cardosin, an aspartic protease from the cardoon Cynara cardunculus L. Action on bovine αs- and β-casein and comparison with chymosin. J. Agric. Food Chem. 44: 42-47.
7. Macedo IQ, Marques P, Delgadillo I (1999) Purification and characterization of a novel plant metalloenzyme. Biothechnol. Tech. (in press).
8. Sarkkinen P, Kalkkinen N, Tilgmann C, Siuro J, Kervinen J, Mikola L (1992) Aspartic proteinase from barley grains is related to mammalian lysossomal cathepsin D. Planta 186: 317-323.
9. Shutov AD, Vaintraub IA (1987) Degradation of storage proteins in germinating seeds. Phytochemistry 26: 1557-1566.
10. Timmermann F, Voudouristsoukala M, Belitz HD (1993) Aspartic proteinases in wheat-flour. I. Characterization. Z. Lebensm. Unters. Forsch. 196: 5-11.
11. Yang JF, Malek L. (1991) Purification of a dry pea seed proteinolytic enzyme complex. Phytochemistry 30: 2487-2491.
12. Zhang N, Jones B (1999) Polymorphism of aspartic proteinases in resting and germinated barley seeds. Cereal Chem. 76: 134-138.