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Volumn 73, Issue 12, 1999, Pages 10029-10039

An epitope of the Semliki Forest virus fusion protein exposed during virus-membrane fusion

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; ARGININE; EPITOPE; GLYCINE; HYBRID PROTEIN; MONOCLONAL ANTIBODY; PROTEIN SUBUNIT; VIRUS PROTEIN;

EID: 0032758908     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.73.12.10029-10039.1999     Document Type: Article
Times cited : (35)

References (49)
  • 1
    • 0021686879 scopus 로고
    • Identification of distinct antigenic determinants on Semliki Forest virus by using monoclonal antibodies with different antiviral activities
    • Boera, W. A. M., T. Harmsen, J. Vinje, B. J. Benaissa-Trouw, C. A. Kraaijeveld, and H. Snippe. 1984. Identification of distinct antigenic determinants on Semliki Forest virus by using monoclonal antibodies with different antiviral activities. J. Virol. 52:575-582.
    • (1984) J. Virol. , vol.52 , pp. 575-582
    • Boera, W.A.M.1    Harmsen, T.2    Vinje, J.3    Benaissa-Trouw, B.J.4    Kraaijeveld, C.A.5    Snippe, H.6
  • 2
    • 0019887744 scopus 로고
    • Phase separation of integral membrane proteins in Triton X-114 solution
    • Bordier, C. 1981. Phase separation of integral membrane proteins in Triton X-114 solution. J. Biol. Chem. 256:1604-1607.
    • (1981) J. Biol. Chem. , vol.256 , pp. 1604-1607
    • Bordier, C.1
  • 3
    • 0027509351 scopus 로고
    • Membrane fusion of Semliki Forest virus in a model system: Correlation between fusion kinetics and structural changes in the envelope glycoprotein
    • Bron, R., J. M. Wahlberg, H. Garoff, and J. Wilschut. 1993. Membrane fusion of Semliki Forest virus in a model system: correlation between fusion kinetics and structural changes in the envelope glycoprotein. EMBO J. 12:693-701.
    • (1993) EMBO J. , vol.12 , pp. 693-701
    • Bron, R.1    Wahlberg, J.M.2    Garoff, H.3    Wilschut, J.4
  • 8
    • 0028935439 scopus 로고
    • Mutations in the putative fusion peptide of Semliki Forest virus affect spike protein oligomerization and virus assembly
    • Duffus, W. A., P. Levy-Mintz, M. R. Klimjack, and M. Kielian. 1995. Mutations in the putative fusion peptide of Semliki Forest virus affect spike protein oligomerization and virus assembly. J. Virol. 69:2471-2479.
    • (1995) J. Virol. , vol.69 , pp. 2471-2479
    • Duffus, W.A.1    Levy-Mintz, P.2    Klimjack, M.R.3    Kielian, M.4
  • 9
    • 0025281122 scopus 로고
    • A conformational change in Sindbis virus glycoproteins E1 and E2 is detected at the plasma membrane as a consequence of early virus-cell interaction
    • Flynn, D. C., W. J. Meyer, J. M. Mackenzie, and R. E. Johnston. 1990. A conformational change in Sindbis virus glycoproteins E1 and E2 is detected at the plasma membrane as a consequence of early virus-cell interaction. J. Virol. 64:3643-3653.
    • (1990) J. Virol. , vol.64 , pp. 3643-3653
    • Flynn, D.C.1    Meyer, W.J.2    Mackenzie, J.M.3    Johnston, R.E.4
  • 10
    • 0029052283 scopus 로고
    • Low pH induces swiveling of the glycoprotein heterodimers in the Semliki Forest virus spike complex
    • Fuller, S. D., J. A. Berriman, S. J. Butcher, and B. E. Gowen. 1995. Low pH induces swiveling of the glycoprotein heterodimers in the Semliki Forest virus spike complex. Cell 81:715-725.
    • (1995) Cell , vol.81 , pp. 715-725
    • Fuller, S.D.1    Berriman, J.A.2    Butcher, S.J.3    Gowen, B.E.4
  • 11
    • 0019157110 scopus 로고
    • The capsid protein of Semliki Forest virus has clusters of basic amino acids and prolines in its amino-terminal region
    • Garoff, H., A.-M. Frischauf, K. Simons, H. Lehrach, and H. Delius. 1980. The capsid protein of Semliki Forest virus has clusters of basic amino acids and prolines in its amino-terminal region. Proc. Natl. Acad. Sci. USA 77: 6376-6380.
    • (1980) Proc. Natl. Acad. Sci. USA , vol.77 , pp. 6376-6380
    • Garoff, H.1    Frischauf, A.-M.2    Simons, K.3    Lehrach, H.4    Delius, H.5
  • 12
    • 0019300295 scopus 로고
    • Nucleotide sequence of cDNA coding for Semliki Forest virus membrane glycoproteins
    • Garoff, H., A.-M. Frischauf, K. Simons, H. Lehrach, and H. Delius. 1980. Nucleotide sequence of cDNA coding for Semliki Forest virus membrane glycoproteins. Nature 288:236-241.
    • (1980) Nature , vol.288 , pp. 236-241
    • Garoff, H.1    Frischauf, A.-M.2    Simons, K.3    Lehrach, H.4    Delius, H.5
  • 13
    • 0031969430 scopus 로고    scopus 로고
    • fus-1, a pH-shift mutant of Semliki Forest virus, acts by altering spike subunit interactions via a mutation in the E2 subunit
    • Glomb-Reinmund, S., and M. Kielian. 1998. fus-1, a pH-shift mutant of Semliki Forest virus, acts by altering spike subunit interactions via a mutation in the E2 subunit. J. Virol. 72:4281-4287.
    • (1998) J. Virol. , vol.72 , pp. 4281-4287
    • Glomb-Reinmund, S.1    Kielian, M.2
  • 14
    • 0032169169 scopus 로고    scopus 로고
    • The role of low pH and disulfide shuffling in the entry and fusion of Semliki Forest virus and Sindbis virus
    • Glomb-Reinmund, S., and M. Kielian. 1998. The role of low pH and disulfide shuffling in the entry and fusion of Semliki Forest virus and Sindbis virus. Virology 248:372-381.
    • (1998) Virology , vol.248 , pp. 372-381
    • Glomb-Reinmund, S.1    Kielian, M.2
  • 15
    • 0026560161 scopus 로고
    • Introduction of intersubunit disulfide bonds in the membrane-distal region of the influenza hemagglutinin abolishes membrane fusion activity
    • Godley, L., J. Pfeifer, D. Steinhauer, B. Ely, G. Shaw, R. Kaufmann, E. Suchanek, C. Pabo, J. J. Skehel, D. C. Wiley, and S. Wharton. 1992. Introduction of intersubunit disulfide bonds in the membrane-distal region of the influenza hemagglutinin abolishes membrane fusion activity. Cell 68:635-645.
    • (1992) Cell , vol.68 , pp. 635-645
    • Godley, L.1    Pfeifer, J.2    Steinhauer, D.3    Ely, B.4    Shaw, G.5    Kaufmann, R.6    Suchanek, E.7    Pabo, C.8    Skehel, J.J.9    Wiley, D.C.10    Wharton, S.11
  • 16
    • 0022450456 scopus 로고
    • A new mechanism for the neutralization of enveloped viruses by antiviral antibody
    • Gollins, S. W., and J. S. Porterfield. 1986. A new mechanism for the neutralization of enveloped viruses by antiviral antibody. Nature 321:244-246.
    • (1986) Nature , vol.321 , pp. 244-246
    • Gollins, S.W.1    Porterfield, J.S.2
  • 17
    • 0029257245 scopus 로고
    • Structural characterization of viral fusion proteins
    • Hughson, F. M. 1995. Structural characterization of viral fusion proteins. Curr. Biol. 5:265-274.
    • (1995) Curr. Biol. , vol.5 , pp. 265-274
    • Hughson, F.M.1
  • 18
    • 0031882119 scopus 로고    scopus 로고
    • Fusion of influenza virus with the endosomal membrane is inhibited by monoclonal antibodies to defined epitopes on the hemagglutinin
    • Imai, M., K. Sugimoto, K. Okazaki, and H. Kida. 1998. Fusion of influenza virus with the endosomal membrane is inhibited by monoclonal antibodies to defined epitopes on the hemagglutinin. Virus Res. 53:129-139.
    • (1998) Virus Res. , vol.53 , pp. 129-139
    • Imai, M.1    Sugimoto, K.2    Okazaki, K.3    Kida, H.4
  • 19
    • 0027487986 scopus 로고
    • Role of spike protein conformational changes in fusion of Semliki Forest virus
    • Justman, J., M. R. Klimjack, and M. Kielian. 1993. Role of spike protein conformational changes in fusion of Semliki Forest virus. J. Virol. 67:7597-7607.
    • (1993) J. Virol. , vol.67 , pp. 7597-7607
    • Justman, J.1    Klimjack, M.R.2    Kielian, M.3
  • 20
    • 0029108852 scopus 로고
    • Membrane fusion and the alphavirus life cycle
    • Kielian, M. 1995. Membrane fusion and the alphavirus life cycle. Adv. Virus Res. 45:113-151.
    • (1995) Adv. Virus Res. , vol.45 , pp. 113-151
    • Kielian, M.1
  • 21
    • 85060773493 scopus 로고    scopus 로고
    • Specific role for lipids in virus fusion and exit: Examples from the alphaviruses
    • in press
    • Kielian, M., P. K. Chatterjee, D. L. Gibbons, and Y. E. Lu. Specific role for lipids in virus fusion and exit: Examples from the alphaviruses. Subcell. Biochem., in press.
    • Subcell. Biochem.
    • Kielian, M.1    Chatterjee, P.K.2    Gibbons, D.L.3    Lu, Y.E.4
  • 22
    • 0022273625 scopus 로고
    • pH-induced alterations in the fusogenic spike protein of Semliki Forest virus
    • Kielian, M., and A. Helenius. 1985. pH-induced alterations in the fusogenic spike protein of Semliki Forest virus. J. Cell Biol. 101:2284-2291.
    • (1985) J. Cell Biol. , vol.101 , pp. 2284-2291
    • Kielian, M.1    Helenius, A.2
  • 23
    • 0025165605 scopus 로고
    • Biosynthesis, maturation, and acid-activation of the Semliki Forest virus fusion protein
    • Kielian, M., S. Jungerwirth, K. U. Sayad, and S. DeCandido. 1990. Biosynthesis, maturation, and acid-activation of the Semliki Forest virus fusion protein. J. Virol. 64:4614-4624.
    • (1990) J. Virol. , vol.64 , pp. 4614-4624
    • Kielian, M.1    Jungerwirth, S.2    Sayad, K.U.3    DeCandido, S.4
  • 24
    • 0029840915 scopus 로고    scopus 로고
    • Mechanisms of mutations inhibiting fusion and infection by Semliki Forest virus
    • Kielian, M., M. R. Klimjack, S. Ghosh, and W. A. Duffus. 1996. Mechanisms of mutations inhibiting fusion and infection by Semliki Forest virus. J. Cell Biol. 134:863-872.
    • (1996) J. Cell Biol. , vol.134 , pp. 863-872
    • Kielian, M.1    Klimjack, M.R.2    Ghosh, S.3    Duffus, W.A.4
  • 26
    • 0028152964 scopus 로고
    • Membrane and protein interactions of a soluble form of the Semliki Forest virus fusion protein
    • Klimjack, M. R., S. Jeffrey, and M. Kielian. 1994. Membrane and protein interactions of a soluble form of the Semliki Forest virus fusion protein. J. Virol. 68:6940-6946.
    • (1994) J. Virol. , vol.68 , pp. 6940-6946
    • Klimjack, M.R.1    Jeffrey, S.2    Kielian, M.3
  • 27
    • 0032930413 scopus 로고    scopus 로고
    • The cholesterol requirement for Sindbis virus entry and exit and characterization of a spike protein region involved in cholesterol dependence
    • Lu, Y. E., T. Cassese, and M. Kielian. 1999. The cholesterol requirement for Sindbis virus entry and exit and characterization of a spike protein region involved in cholesterol dependence. J. Virol. 73:4272-4278.
    • (1999) J. Virol. , vol.73 , pp. 4272-4278
    • Lu, Y.E.1    Cassese, T.2    Kielian, M.3
  • 28
    • 15844427939 scopus 로고    scopus 로고
    • Wortmannin-sensitive trafficking pathways in Chinese hamster ovary cells - Differential effects on endocytosis and lysosomal sorting
    • Martys, J. L., C. Wjasow, D. M. Gangi, M. C. Kielian, T. E. McGraw, and J. M. Backer. 1996. Wortmannin-sensitive trafficking pathways in Chinese hamster ovary cells - differential effects on endocytosis and lysosomal sorting. J. Biol. Chem. 271:10953-10962.
    • (1996) J. Biol. Chem. , vol.271 , pp. 10953-10962
    • Martys, J.L.1    Wjasow, C.2    Gangi, D.M.3    Kielian, M.C.4    McGraw, T.E.5    Backer, J.M.6
  • 29
    • 0026655252 scopus 로고
    • Conformational alteration of Sindbis virion glycoproteins induced by heat, reducing agents, or low pH
    • Meyer, W. J., S. Gidwitz, V. K. Avers, R. J. Schoepp, and R. E. Johnston. 1992. Conformational alteration of Sindbis virion glycoproteins induced by heat, reducing agents, or low pH. J. Virol. 66:3504-3513.
    • (1992) J. Virol. , vol.66 , pp. 3504-3513
    • Meyer, W.J.1    Gidwitz, S.2    Avers, V.K.3    Schoepp, R.J.4    Johnston, R.E.5
  • 30
    • 0027219587 scopus 로고
    • Structural rearrangement of infecting Sindbis virions at the cell surface: Mapping of newly accessible epitopes
    • Meyer, W. J., and R. E. Johnston. 1993. Structural rearrangement of infecting Sindbis virions at the cell surface: mapping of newly accessible epitopes. J. Virol. 67:5117-5125.
    • (1993) J. Virol. , vol.67 , pp. 5117-5125
    • Meyer, W.J.1    Johnston, R.E.2
  • 31
    • 0025094320 scopus 로고
    • Structural and serological evidence for a novel mechanism of antigenic variation in foot-and-mouth disease virus
    • Parry, N., G. Fox, D. Rowlands, F. Brown, E. Fry, R. Acharya, D. Logan, and D. Stuart. 1990. Structural and serological evidence for a novel mechanism of antigenic variation in foot-and-mouth disease virus. Nature 347:569-572.
    • (1990) Nature , vol.347 , pp. 569-572
    • Parry, N.1    Fox, G.2    Rowlands, D.3    Brown, F.4    Fry, E.5    Acharya, R.6    Logan, D.7    Stuart, D.8
  • 32
    • 0026022952 scopus 로고
    • Cholesterol is required for infection by Semliki Forest virus
    • Phalen, T., and M. Kielian. 1991. Cholesterol is required for infection by Semliki Forest virus. J. Cell Biol. 112:615-623.
    • (1991) J. Cell Biol. , vol.112 , pp. 615-623
    • Phalen, T.1    Kielian, M.2
  • 33
    • 0026567676 scopus 로고
    • Membrane fusion process of Semliki Forest virus. II. Cleavage-dependent reorganization of the spike protein complex controls virus entry
    • Salminen, A., J. M. Wahlberg, M. Lobigs, P. Liljeström, and H. Garoff. 1992. Membrane fusion process of Semliki Forest virus. II. Cleavage-dependent reorganization of the spike protein complex controls virus entry. J. Cell Biol. 116:349-357.
    • (1992) J. Cell Biol. , vol.116 , pp. 349-357
    • Salminen, A.1    Wahlberg, J.M.2    Lobigs, M.3    Liljeström, P.4    Garoff, H.5
  • 34
    • 0021066631 scopus 로고
    • Protective monoclonal antibodies define maturational and pH-dependent antigenic changes in sindbis virus E1 glycoprotein
    • Schmaljohn, A. L., K. M. Kokubun, and G. A. Cole. 1983. Protective monoclonal antibodies define maturational and pH-dependent antigenic changes in Sindbis virus E1 glycoprotein. Virology 130:144-154.
    • (1983) Virology , vol.130 , pp. 144-154
    • Schmaljohn, A.L.1    Kokubun, K.M.2    Cole, G.A.3
  • 35
    • 0024564284 scopus 로고
    • Acidification of endosome subpopulations in wild-type Chinese hamster ovary cells and temperature-sensitive acidification-defective mutants
    • Schmid, S. L., R. Fuchs, M. Kielian, A. Helenius, and I. Mellman. 1989. Acidification of endosome subpopulations in wild-type Chinese hamster ovary cells and temperature-sensitive acidification-defective mutants. J. Cell Biol. 108:1291-1300.
    • (1989) J. Cell Biol. , vol.108 , pp. 1291-1300
    • Schmid, S.L.1    Fuchs, R.2    Kielian, M.3    Helenius, A.4    Mellman, I.5
  • 36
    • 0032431055 scopus 로고    scopus 로고
    • Coiled coils in both intracellular vesicle and viral membrane fusion
    • Skehel, J. J., and D. C. Wiley. 1998. Coiled coils in both intracellular vesicle and viral membrane fusion. Cell 95:871-874.
    • (1998) Cell , vol.95 , pp. 871-874
    • Skehel, J.J.1    Wiley, D.C.2
  • 37
    • 0022620217 scopus 로고
    • Antibody-selected variation and reversion in Sindbis virus neutralization epitopes
    • Stec, D. S., A. Waddell, C. S. Schmaljohn, B. A. Cole, and A. L. Schmaljohn. 1986. Antibody-selected variation and reversion in Sindbis virus neutralization epitopes. J. Virol. 57:715-720.
    • (1986) J. Virol. , vol.57 , pp. 715-720
    • Stec, D.S.1    Waddell, A.2    Schmaljohn, C.S.3    Cole, B.A.4    Schmaljohn, A.L.5
  • 38
    • 0029022326 scopus 로고
    • Human hepatoma cell mutant defective in cell surface protein trafficking
    • Stockert, R. J., B. Potvin, L. Tao, P. Stanley, and A. W. Wolkoff. 1995. Human hepatoma cell mutant defective in cell surface protein trafficking. J. Biol. Chem. 279:16107-16113.
    • (1995) J. Biol. Chem. , vol.279 , pp. 16107-16113
    • Stockert, R.J.1    Potvin, B.2    Tao, L.3    Stanley, P.4    Wolkoff, A.W.5
  • 39
    • 0025945880 scopus 로고
    • Identification of antigenically important domains in the glycoproteins of Sindbis virus by analysis of antibody escape variants
    • Strauss, E. G., D. S. Stec, A. L. Schmaljohn, and J. H. Strauss. 1991. Identification of antigenically important domains in the glycoproteins of Sindbis virus by analysis of antibody escape variants. J. Virol. 65:4654-4664.
    • (1991) J. Virol. , vol.65 , pp. 4654-4664
    • Strauss, E.G.1    Stec, D.S.2    Schmaljohn, A.L.3    Strauss, J.H.4
  • 40
    • 0028088152 scopus 로고
    • The alphaviruses: Gene expression, replication, and evolution
    • Strauss, J. H., and E. G. Strauss. 1994. The alphaviruses: gene expression, replication, and evolution. Microbiol. Rev. 58:491-562.
    • (1994) Microbiol. Rev. , vol.58 , pp. 491-562
    • Strauss, J.H.1    Strauss, E.G.2
  • 41
    • 15644375941 scopus 로고    scopus 로고
    • An antibody which binds to the membrane-proximal end of influenza virus haemagglutinin (H3 subtype) inhibits the low-pH-induced conformational changes and cell-cell fusion but does not neutralize virus
    • Vanlandschoot, P., E. Beirnaert, B. Barrere, B. Millar, S. Wharton, W. M. Jou, and W. Fiers. 1998. An antibody which binds to the membrane-proximal end of influenza virus haemagglutinin (H3 subtype) inhibits the low-pH-induced conformational changes and cell-cell fusion but does not neutralize virus. J. Gen. Virol. 79:1781-1791.
    • (1998) J. Gen. Virol. , vol.79 , pp. 1781-1791
    • Vanlandschoot, P.1    Beirnaert, E.2    Barrere, B.3    Millar, B.4    Wharton, S.5    Jou, W.M.6    Fiers, W.7
  • 42
    • 0031919431 scopus 로고    scopus 로고
    • A single point mutation controls the cholesterol dependence of Semliki Forest virus entry and exit
    • Vashishtha, M., T. Phalen, M. T. Marquardt, J. S. Ryu, A. C. Ng, and M. Kielian. 1998. A single point mutation controls the cholesterol dependence of Semliki Forest virus entry and exit. J. Cell Biol. 140:91-99.
    • (1998) J. Cell Biol. , vol.140 , pp. 91-99
    • Vashishtha, M.1    Phalen, T.2    Marquardt, M.T.3    Ryu, J.S.4    Ng, A.C.5    Kielian, M.6
  • 43
    • 0024455501 scopus 로고
    • The heterodimeric association between the membrane proteins of Semliki Forest virus changes its sensitivity to low pH during virus maturation
    • Wahlberg, J. M., W. A. M. Boere, and H. Garoff. 1989. The heterodimeric association between the membrane proteins of Semliki Forest virus changes its sensitivity to low pH during virus maturation. J. Virol. 63:4991-4997.
    • (1989) J. Virol. , vol.63 , pp. 4991-4997
    • Wahlberg, J.M.1    Boere, W.A.M.2    Garoff, H.3
  • 44
    • 0026495818 scopus 로고
    • Membrane fusion of Semliki Forest virus involves homotrimers of the fusion protein
    • Wahlberg, J. M., R. Bron, J. Wilschut, and H. Garoff. 1992. Membrane fusion of Semliki Forest virus involves homotrimers of the fusion protein. J. Virol. 66:7309-7318.
    • (1992) J. Virol. , vol.66 , pp. 7309-7318
    • Wahlberg, J.M.1    Bron, R.2    Wilschut, J.3    Garoff, H.4
  • 45
    • 0026584727 scopus 로고
    • Membrane fusion process of Semliki Forest virus. I. Low pH-induced rearrangement in spike protein quaternary structure precedes virus penetration into cells
    • Wahlberg, J. M., and H. Garoff. 1992. Membrane fusion process of Semliki Forest virus. I. Low pH-induced rearrangement in spike protein quaternary structure precedes virus penetration into cells. J. Cell Biol. 116:339-348.
    • (1992) J. Cell Biol. , vol.116 , pp. 339-348
    • Wahlberg, J.M.1    Garoff, H.2
  • 46
    • 0020530076 scopus 로고
    • Changes in the antigenicity of the hemagglutinin molecule of H3 influenza virus at acidic pH
    • Webster, R. G., L. E. Brown, and D. C. Jackson. 1983. Changes in the antigenicity of the hemagglutinin molecule of H3 influenza virus at acidic pH. Virology 126:587-599.
    • (1983) Virology , vol.126 , pp. 587-599
    • Webster, R.G.1    Brown, L.E.2    Jackson, D.C.3
  • 47
    • 0023574003 scopus 로고
    • Anti-peptide antibodies detect steps in a protein conformational change: Low-pH activation of the influenza hemagglutinin
    • White, J. M., and I. A. Wilson. 1987. Anti-peptide antibodies detect steps in a protein conformational change: low-pH activation of the influenza hemagglutinin. J. Cell Biol. 105:2887-2896.
    • (1987) J. Cell Biol. , vol.105 , pp. 2887-2896
    • White, J.M.1    Wilson, I.A.2
  • 48
    • 0023082135 scopus 로고
    • The structure and function of the hemagglutinin membrane glycoprotein of influenza virus
    • Wiley, D. C., and J. J. Skehel. 1987. The structure and function of the hemagglutinin membrane glycoprotein of influenza virus. Annu. Rev. Biochem. 56:365-394.
    • (1987) Annu. Rev. Biochem. , vol.56 , pp. 365-394
    • Wiley, D.C.1    Skehel, J.J.2
  • 49
    • 0029201982 scopus 로고
    • Fusion of Semliki Forest virus with cholesterol-containing liposomes at low pH: A specific requirement for sphingolipids
    • Wilschut, J., J. Corver, J. L. Nieva, R. Bron, L. Moesby, K. C. Reddy, and R. Bittman. 1995. Fusion of Semliki Forest virus with cholesterol-containing liposomes at low pH: a specific requirement for sphingolipids. Mol. Membr. Biol. 12:143-149.
    • (1995) Mol. Membr. Biol. , vol.12 , pp. 143-149
    • Wilschut, J.1    Corver, J.2    Nieva, J.L.3    Bron, R.4    Moesby, L.5    Reddy, K.C.6    Bittman, R.7


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