Laundry detergent effectively preserves amphibian and reptile blood and tissue for DNA isolation

Herpetological Review

Volumn 30, Issue 2, 1999, Pages 80-82

  Kuch, Ulrich ^a   Pfenninger, Markus ^a   Bahl, Andreas ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA ISOLATION; PRESERVATIVE;

EID: 0032757644     PISSN: 0018084X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. ARCTANDER, P. 1988. Comparative studies of avian DNA by restriction fragment length polymorphism analysis: Convenient procedures based on blood samples from live birds. J. Ornithologie 129:205-216.
2. ASIH, HL, AND SSAR. 1987. Guidelines for Use of Live Amphibians and Reptiles in Field Research. (Available from Robert D. Aldridge, SSAR Publications Secretary, Dept. Biology, St. Louis University, St. Louis, Missouri 63130, USA)
3. BAHL, A., AND M. PFENNINGER. 1996. A rapid method of DNA isolation using laundry detergent. Nucleic Acids Research 24:1587-1588.
4. DESSAUER, H. C., AND M. S. HAFNER (EDS.). 1984. Collections of frozen tissues: value, management, field and laboratory procedures, and directory of existing collections. Association of Systematics Collections, Univ. Kansas Press, Lawrence.
5. _, C. J. COLE, AND M. S. HAFNER. 1990. Collection and storage of tissues. In D. M. Hillis and C. Moritz (eds.), Molecular Systematics, pp. 25-41. Sinauer, Sunderland.
6. DONNELLY M. A., C. GUYER, J. E. JUTERBOCK, AND R. A. ALFORD. 1994. Techniques for marking amphibians. In W. R. Heyer, M. A. Donnelly, R. W. McDiarmid, L. A. C. Hayek, and M. S. Forster (eds.). Measuring and Monitoring Biological Diversity: Standard Methods for Amphibians, pp. 277-284. Smithsonian Institution Press, Washington, D.C.
7. FERNER, J. W. 1979. A review of marking techniques for amphibians and reptiles. SSAR Herpetol. Circ. 9:1-41.
8. GONSER, R. A. , AND R. V. COLLURA. 1996. Waste not, want not: toe-clips as a source of DNA. J. Herpetol. 30:445-447.
9. SAMBROOK, J., E. F. FRITSCH, AND T. MANIATIS. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.