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Volumn 121, Issue 41, 1999, Pages 9748-9749

Isoniazid oxidation by Mycobacterium tuberculosis KatG: A role for superoxide which correlates with isoniazid susceptibility [14]

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; CATALASE; CYTOCHROME C; CYTOCHROME P450; ISONIAZID; KATG; MANGANESE PEROXIDASE; PEROXIDASE; PLUMBAGIN; SUPEROXIDE; SUPEROXIDE DISMUTASE; UNCLASSIFIED DRUG; XANTHINE; XANTHINE OXIDASE;

EID: 0032757045     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja992590a     Document Type: Letter
Times cited : (59)

References (32)
  • 1
    • 0344105477 scopus 로고    scopus 로고
    • World Health Organization, 1998
    • World Health Organization, 1998.
  • 17
    • 0344076418 scopus 로고    scopus 로고
    • note
    • 11 Reactions were conducted at 37 °C in 50 mM sodium phosphate buffer (pH 7.5) and samples removed at selected times. HPLC was done using a Rainin Dynamax C-18 column (4.6 × 150 mm, 100 Å, 5 μm, Varian Instruments) with a linear gradient of 5-80% acetonitrile (v/v) in 50 mM ammonium acetate (pH 7.0) over 30 min. Products were monitored at 260 nm. Initial velocities were determined by integration of isoniazid peak areas.
  • 18
    • 0344076417 scopus 로고    scopus 로고
    • note
    • 3,10 KatG- and KatG(S315T)-catalyzed INH oxidation gave isonicotinic acid (major) and isonicotinamide (minor) as products. NH oxidation by both enzymes also produced the corresponding acid and amide.
  • 19
    • 0014691242 scopus 로고
    • -1 near neutral pH. Therefore, a xanthine/xanthine oxidase superoxide-generating system was utilized to provide a constant, low superoxide level. McCord, J. M.; Fridovich, I. J. Biol. Chem. 1969, 244, 6049-6055.
    • (1969) J. Biol. Chem. , vol.244 , pp. 6049-6055
    • McCord, J.M.1    Fridovich, I.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.