Cisplatin-digoxigenin mRNA labeling for nonradioactive detection of mRNA hybridized onto nucleic acid cDNA arrays

BioTechniques

Volumn 27, Issue 5, 1999, Pages 1064-1067

  Hoevel, Thorsten ^a   Holz, H ^b   Kubbies, M ^a  

^a ROCHE INNOVATION CENTER MUNICH   (Germany)

^b ROCHE DIAGNOSTICS GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CISPLATIN; COMPLEMENTARY DNA; DIGOXIGENIN; MESSENGER RNA; NUCLEIC ACID;

ARTICLE; FIBROBLAST; GENE EXPRESSION; HUMAN; HUMAN CELL; HYBRIDIZATION; ISOTOPE LABELING; RADIOACTIVITY; TECHNIQUE;

EID: 0032755957     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99275pf01     Document Type: Article

References (14)

1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl. 1998. Current Protocols in Molecular Biology. Greene Publishing Associates and John Wiley & Sons. New York.
2. Brown, P.O. and D. Botstein. 1999. Exploring the new world of the genome with DNA microarrays. Nat. Genet. 21:33-37.
3. Chee, M., R. Yang, E. Hubell, A. Berno, X.C. Huang, D. Stern, J. Winkler, D.J. Lockhart et al. 1996. Accessing genetic information with high-density DNA arrays. Science 274:610-614.
4. DeRisi, J., L. Penland, O.O. Brown, M.L. Bittner, P.S. Meltzer, M. Ray, Y. Chen, Y.A. Su et al. 1996. Use of a cDNA microarray to analyse gene expression patterns in human cancer. Nat. Genet. 14:457-460.
5. Duggan, D.J., M. Bittner, Y. Chen, P. Meltzer and J.M. Trent. 1999. Expression profiling using cDNA microarrays. Nat. Genet. 21:10-14.
6. Heller, R.A., M. Schena, A. Chai, D. Shalon, T. Dedilion, J. Gilmore, D.E. Woolley et al. 1997. Discovery and analysis of inflammatory disease-related genes using cDNA microarrays. Proc. Natl. Acad. Sci. USA 94:2150-2155.
7. Holz, H., C. Goldstein, R. Rein and H.J. Hoeltke. 1997. DIG-ChemLink labeling and Detection Kit. Biochemica 3:26-27.
8. Lennon, G.G. and H. Lehrach. 1991. Hybridization analyses of arrayed cDNA libraries. Trends Genet. 7:314-317.
9. Lipshutz, R.J., S.P.A. Fodor, T.R. Gingeras and D.J. Lockhart. 1999. High density synthetic oligonucleotide arrays. Nat. Genet. 21:20-24.
10. Lockhart, D.J., H. Dong, M.C. Byrne, M.T. Follettie Gallo, M.S. Chee, M. Mittmann, C. Wang, M. Kobayashi et al. 1996. Expression monitoring by hybridization to high-density oligonucleotide arrays. Nat. Biotechnol. 14:1649.
11. Nguyen, C., D. Rocha, S. Granjeaud, M. Baldit, K. Bernard, P. Naquet and R.B. Jordan. 1995. Differential gene expression in the murine thymus assayed by quantitative hybridization of arrayed cDNA clones. Genomics 1:207-216.
12. Pietu, G., O. Alibert, V. Guichard, B. Lamy, F. Bois, E. Leroy, R. Mariage-Sampson, R. Houlgatte et al. 1996. Novel gene transcripts preferentially expressed in human muscles revealed by quantitative hybridization of a high density cDNA array. Genome Res. 6:492-503.
13. Planitzer, A.S., A.W. Machl, M. Rueckels and M. Kubbies. 1998. Identification of a novel c-DNA overexpressed in Fanconi's anemia fibroblasts partially homologous to a putative L-3-phosphoserine-phosphatase. Gene 210:297-306.
14. Van Belkum, A., E. Linkels, T. Jelsma, T.M. van den Berg and W. Quint. 1994. Non-isotopic labeling of DNA by newly developed hapten-containing platinum compounds. BioTechniques 16:148-154.