A novel assay for allelic discrimination that combines the fluorogenic 5' nuclease polymerase chain reaction (TaqMan®) and mismatch amplification mutation assay

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 430, Issue 1, 1999, Pages 1-12

  Glaab, Warren E ^a   Skopek, Thomas R ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Allelic discrimination; HPRT; MAMA; Mismatch amplification; TaqMan

Indexed keywords

HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; NUCLEASE;

ALLELE; ARTICLE; BASE MISPAIRING; BIOASSAY; CELL LINE; EXON; FLUORESCENCE; GENE AMPLIFICATION; HUMAN; HUMAN CELL; LYMPHOBLASTOID CELL; MUTANT; MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ALLELES; BASE PAIR MISMATCH; CELL LINE, TRANSFORMED; DNA MUTATIONAL ANALYSIS; DNA PROBES; EXONS; FLUORESCENT DYES; HUMANS; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; PLACENTA; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; TAQ POLYMERASE;

EID: 0032751267     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(99)00147-5     Document Type: Article

References (8)

1. Parsons B.L., Heflich R.H. Genotypic selection methods for the direct analysis of point mutations. Mutat. Res. 387:1997;97-121.
2. Newton C.R., Graham A., Heptinstall L.E., Powell S.J., Summers C., Kalsheker N., Smith J.C., Markham A.F. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res. 17:1989;2503-2516.
3. Orou A., Fechner B., Utermann G., Menzel H.J. Allele-specific competitive blocker PCR: one-step method with applicability to pool screening. Human Mutation. 6:1995;163-169.
4. Tyagi S., Kramer F.R. Molecular beacons: probes that fluoresce upon hybridization. Nature Biotech. 14:1996;303-308.
5. De Francesco L. The next new wave in genome analysis. Scientist. 12:1998;16-20.
6. Heid C.A., Stevens J., Livak K.J., Williams P.M. Real time quantitive PCR. Genome Res. 6:1996;986-994.
7. Cha R.S., Zarbl H., Keohavong P., Thilly W.G. Mismatch amplification mutation assay (MAMA): application to the c-H-ras gene. PCR Methods and Applications. 2:1992;14-20.
8. Jolly D., Okayama H., Berg P., Esty A., Filpula D., Bohlen P., Johnson G., Shively J.E., Hunkapillar T., Friedmann T. Isolation and characterization of a full-length expressible cDNA for human hprt. Proc. Natl. Acad. Sci. U.S.A. 80:1983;477-481.