The application of single cell gel electrophoresis or comet assay to human monitoring studies

Salud Publica de Mexico

Volumn 41, Issue SUPPL. 2, 1999, Pages

  Valverde, Mahara ^b   Ostrosky Wegman, Patricia ^b   Rojas Del Castillo, Emilio ^a   Fortoul, Teresa ^b   Meneses, Fernando ^b   Ramírez, Matiana ^b   Díaz Barriga, Fernando ^b   Cebrian, Mariano ^b  

^a UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^b NONE

Author keywords

Biological markers; Single cell gel electrophoresis assay

Indexed keywords

BIOLOGICAL MARKER;

ARTICLE; DNA STRAND BREAKAGE; ENVIRONMENTAL EXPOSURE; EXPERIENCE; GEL ELECTROPHORESIS; GENOTOXICITY; MONITORING; OCCUPATIONAL EXPOSURE; POPULATION RESEARCH;

EID: 0032750532     PISSN: 00363634     EISSN: None     Source Type: Journal    
DOI: 10.1590/S0036-36341999000800007     Document Type: Article

References (26)

1. Albertini RJ, Robison SH. Human population monitoring. In: Li AP, Heflich RH, eds. Genetic Toxicology. Boca Ratón:CRC Press, 1991;375:420.
2. Committe on Biological Markers of the National Research Council. Biol Markeers in Environ Health Res 1987;74:3-9.
3. Grandjean P, Brown SS, Reavey P, Young DS. Biomarkers of chemical exposure: State of Art. Clin Chem 1994;40(7):1360-1362.
4. Tucker JD, Auletta A, Cimino MC, Dearfield KL, Jacobson-Kram D, Tice RR et al. Sister chromatid exchange: Second report of the Gene-Tox Program. Mutat Res 1993;297:101-180.
5. Anwar WA. Monitoring of human population at risk by different cytogenetic end points. Environ Health Perspect 1994;102 suppl 4:131-134.
6. Ostrosky-Wegman P, Gonsebatt ME. Environmental toxicants in developing countries. Environ Health Perspect 1996;104 (suppl 3):599-602.
7. Norppa H, Luomahaara S, Heikanen H, Roth S, Sorsa M, Renzi L et al. Micronucleus assay in lymphocytes as a tool to biomonitor human exposure to aneuploidogens and clastogens. Environ Health Perspect 1993;101 suppl 3:139-143.
8. Tice RR. The single cell gel/comet assay. In: Phillips DH, Venitt S, eds. Environmental mutagenesis. Oxford, U.K.: Bios Scientific Publishers LTD, 1995.
9. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184-191.
10. McKelvey-Martin VJ, Green MHL, Schmezer P, Pool-Zobel BL, De Meo MP, Collins A. The single cell gel electrophoresis (comet assay): An European review. Mutat Res 1993;288:47-63.
11. Fairbairn DW, Olive PL, O'Neill KL. The comet assay: An comprehensive review. Mutat Res 1995;339:37-59.
12. Hartmann A, Speit G. Comparative investigations of the genotoxic effects of metals in the single cell gel (SCG) assay and the sister chromatid exchange (SCE) test. Env Mol Mutagen 1994;23:299-305.
13. Comet Workshop. Croydon, UK May 8-10, 1997.
14. Ross GM, McMillan TJ, Wilcox P, Collins AR. The single cell microgel electrophoresis assay (comet assay): Technical aspects and applications, Reports on the 5th LH Gray Trust Workshop, Institute of Cancer Research, 1994. Mutat Res 1995;337:57-60.
15. Calderón-Garcidueñas L, Osnaya-Brizuela N, Ramírez-Martínez L, Villareal-Calderón A. DNA strand breaks in human nasal respiratory epithelium are induced upon exposure to urban pollution. Environ Health Perspect 1996;104(2):160-167.
16. Rojas E, Valverde M, Sordo M, Ostrosky-Wegman P. DNA damage in exfoliated buccal cells of smokers assessed by the single cell gel electrophoresis assay. Mutat Res 1996;370:115-120.
17. Cox DH. Arsine evolution-electrothermal atomic absorption method for the determination of nanogram levels of total arsenic in urine and water. J Anal Toxicol 1980;4:207-211.
18. Bousnes RW, Tawssky HH. On the colorimetric determination of creatinine by the Jaffe reaction. J Biol Chem 1945;158:581.
19. Valverde M, Lopez MC, Lopez Y, Sanchez Y, Fortoul TI, Ostrosky-Wegman P et al. Environ Mol Mutagen 1997;30:147-152.
20. Andreoli C, Leopardi P, Crebelli R. Detection of DNA damage in human lymphocytes by alkaline single cell gel electrophoresis after exposure to benzene or benzene metabolites. Mutat Res 1997;377:95-104.
21. Reese E, Kimbrough RD. Acute toxicity of gasoline and some additives. Environ Health Perspect 1994;101:115-131.
22. Ostrosky-Wegman P, Gonsebatt ME, Montera R, Vega L, Barba H, Espinosa J et al. Lymphocyte proliferation kinetics and genotoxic findings in a pilot study on individuals chronically exposed to arsenic in Mexico. Mutat Res 1991;250:477-482.
23. Gonsebatt ME, Vega L, Salazar AM, Montero R, Guzman P, Blas J et al. Cytogenetic effects in human exposure to arsenic. Mutat Res 1997;386: 219-228.
24. Ramirez P, Eastmond DA, Laclette JP, Ostrosky-Wegman P. Disruption of microtubule assembly and spindle formation as a mechanism for the induction of aneuploid cell by sodium arsenite and vanadium pentoxide. Mutat Res 1997;386:291-298.
25. Salazar AM, Ostrosky-Wegman P, Menendez D, Miranda E, Garcia-Carranca A, Rojas E. Induction of p53 protein expression by sodium arsenite. Mutat Res 1997;381:259-265.
26. Carson RL. La primavera silenciosa. México D.F.: Editorial Grijalbo. 1980:334.