Immobiline-based two-dimensional gel electrophoresis: An optimised protocol for resolution of human colonic mucosal proteins

Electrophoresis

Volumn 20, Issue 17, 1999, Pages 3467-3471

  Chan, Charles ^a,d   Warlow, Robert S ^b   Chapuis, Pierre H ^a   Newland, Ronald C ^c   Bokey, E Leslie ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

^b Immunology Department   (Australia)

^c Department of Microbiology and Infectious Diseases   (Australia)

^d ROYAL NORTH SHORE HOSPITAL   (Australia)

Author keywords

Colonic carcinoma; Detergent solubilisation of proteins; Human colonic mucosa; Immobilised pH gradients; Isoelectric focusing; Two dimensional gel electrophoresis

Indexed keywords

ASCORBIC ACID; ELECTROPHORESIS; FLUORINE COMPOUNDS; SOLUBILITY; UREA;

COLONIC CARCINOMA; COLONIC MUCOSA; DETERGENT SOLUBILIZATION OF PROTEIN; HUMAN COLONIC MUCOSA; IMMOBILIZED PH GRADIENT; ISOELECTRIC FOCUSING; OPTIMIZED PROTOCOL; PH GRADIENTS; SOLUBILISATION; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

PROTEINS;

ASCORBIC ACID; BENZYLSULFONYL FLUORIDE; GLYCEROL; MERCAPTOETHANOL; PROTEIN; TRITON X 100; UREA;

ARTICLE; COLON MUCOSA; HUMAN; HUMAN TISSUE; ISOELECTRIC FOCUSING; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

EID: 0032740531     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1522-2683(19991101)20:17<3467::AID-ELPS3467>3.0.CO;2-9     Document Type: Article

References (12)

1. Bjellqvist, B., Ek, K., Righetti, P. G., Gianazza, E., Görg, A., Westermeier, R., Postel, W., J. Biochem. Biophys. Methods 1982, 8, 134-155.
2. Hochstrasser, D. F., Patchornik, A., Merril, C. R., Anal, Biochem. 1988, 173, 412-423.
3. Hochstrasser, D. F., Merril, C. R., Appl. Theor. Electrophor. 1988, 1, 35-40.
4. Patestos, N. P., Fauth, M., Radola, B. J., Electrophoresis 1988, 9, 488-496.
5. Heukeshoven, J., Dernick, R., in: Radola, B. J. (Ed.), Elektrophorese-Forum '86, Technische Universität, München 1986, pp. 22-27.
6. Reymond, M. A., Sanchez, J.-C., Schneider, C., Rohwer, P., Tortola, S., Hohenberger, W., Kirchner, T., Hochstrasser, D. F., Kockerling, F., Electrophoresis 1997, 18, 622-624.
7. Stulik, J., Bures, J., Jandik, P., Langr, F., Kovarova, H., Macela, A., Electrophoresis 1997, 18, 625-628.
8. Pietta, P., Pocaterra, E., Fiorino, A., Gianazza, E., Righetti, P. G., Electrophoresis 1987, 6, 162-170.
9. Rabilloud, T., Gelfi, C., Bossi, M. L., Righetti, P. G., Electrophoresis 1987, 8, 305-312.
10. Righetti, P. G., Chiari, M., Casale, E., Chiesa, C., Appl. Theor. Electrophor. 1989, 1, 115-121.
11. Altland, K., Becher, P., Banzhoff, A., Electrophoresis 1987, 8, 293-297.
12. Görg, A., Postel, W.,Friedrich, C., Kuick, R., Strahler, J. R., Hanash, S. M., Electrophoresis 1991, 12, 653-658.