Induction of mucin gene expression in human colonic cell lines by PMA is dependent on PKC-ε

American Journal of Physiology - Gastrointestinal and Liver Physiology

Volumn 277, Issue 5 40-5, 1999, Pages

  Hong, D H ^a   Petrovics, G ^b   Anderson, W B ^b   Forstner, J ^a   Forstner, G ^a  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Phorbol 12 myristate 13 acetate; Protein kinase C; Signal transduction

Indexed keywords

MESSENGER RNA; MUCIN; PHORBOL 13 ACETATE 12 MYRISTATE; PROTEIN KINASE C;

ARTICLE; CELL STRAIN HT29; COLON; CONTROLLED STUDY; EXOCYTOSIS; GENE EXPRESSION REGULATION; GENE INDUCTION; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; INTESTINE CELL; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

EID: 0032739875     PISSN: 01931857     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpgi.1999.277.5.g1041     Document Type: Article

References (40)

1. Abraham, C., B. Scaglione-Sewell, S. F. Skarosi, W. Qin, M. Bissonnette, and T. A. Brasitus. Protein kinase C α modulates growth and differentiation in Caco-2 cells. Gastroenterology 114: 503-509, 1998.
2. 1 cells. J. Biol. Chem. 269: 4653-4660, 1994.
3. Carrato, C., C. Balague, C. De Bolos, E. Gonzalez, G. Gambus, J. Planas, J. M. Perini, D. Andreu, and F. X. Real. Differential apomucin expression in normal and neoplastic human gastrointestinal tissues. Gastroenterology 107: 160-172, 1994.
4. Chambers, J. A., and S. Harris. Expression of the cystic fibrosis gene and the major panceatic mucin gene MUC1 in human ductal epithelial cells. J. Cell Sci. 105: 417-422, 1993.
5. Chomzynski, P., and N. Sacchi. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159, 1987.
6. Desseyn, J.-L., V. Guyonnet-Duperat, N. Porchet, J.-P. Aubert, and A. Laine. Human mucin gene MUC5B, the 10.7-kb large central exon encodes various alternate subdomains resulting in a super-repeat. J. Biol. Chem. 272: 3168-3178, 1997.
7. Dufosse, J., N. Porchet, J. Audie, V. Guyonnet Duperat, A. Laine, I. Van-Seuningen, S. Marrakchi, P. Degand, and J. Aubert. Degenerate 87-base-pair tandem repeats create hydrophilic/hydrophobic alternating domains in human mucin peptides mapped to 11p15. Biochem. J. 293: 329-337, 1993.
8. Forstner, G. Signal transduction, packaging and secretion of mucins. Annu. Rev. Physiol. 57: 585-605, 1995.
9. 2+ ionophore. Am. J. Physiol. 264 (Gastrointest. Liver Physiol. 27): G1096-G1102, 1993.
10. Gum, J. R., J. J. L. Ho, W. S. Pratt, J. W. Hicks, A. S. Hill, L. E. Vinall, A. M. Roberton, D. M. Swallow, and Y. S. Kim. Muc3 human intestinal mucin: analysis of gene structure, the carboxyl terminus, and a novel upstream repetitive region. J. Biol. Chem. 272: 26678-26686, 1997.
11. Ho, S. N., H. D. Hunt, R. M. Horton, J. K. Pullen, and L. R. Pease. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77: 51-59, 1989.
12. Hong, D.-H., J. F. Forstner, and G. Forstner. Protein kinase C-∈ is the likely mediator of mucin exocytosis in human colonie cell lines. Am. J. Physiol. 272 (Gastrointest. Liver Physiol. 35): G31-G37, 1997.
13. Kellerer, M., J. Mushack, H. Mischak, and H. U. Haring. Protein kinase C (PKC) ∈ enhances the inhibitory effect of TNFα on insulin signaling in HEK293 cells. FEBS Lett. 418: 119-122, 1997.
14. Khatri, I. A., G. G. Forstner, and J. F. Forstner. The carboxyl-terminal sequence of rat intestinal mucin RMuc3 contains a putative transmembrane region and two EGF-like motifs. Biochim. Biophys. Acta 1326: 7-11, 1997.
15. - secretion in epithelial monolayers of mucus-forming human colon cells (HT-29/B6). Am. J. Physiol. 261 (Cell Physiol. 30): C574-C582, 1991.
16. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685, 1970.
17. Lan, M. S., S. K. Batra, W.-N. Qi, R. S. Metzgar, and M. A. Hollingsworth. Cloning and sequencing of a human pancreatic tumor mucin cDNA. J. Biol. Chem. 265: 15294-15299, 1990.
18. Lehel, C., Z. Olah, G. Jakab, and W. B. Anderson. Protein kinase C∈ is localized to the golgi via its zinc-finger domain and modulates golgi function. Proc. Natl. Acad. Sci. USA 92: 1406-1410, 1995.
19. Lesuffleur, T., N. Porchet, J.-P. Aubert, D. Swallow, J. R. Gum, Y. S. Kim, F. X. Real, and A. Zweibaum. Differential expression of the human mucin genes MUC1 to MUC5 in relation to growth and differentiation of different mucus-secreting HT-29 cell populations. J. Cell Sci. 106: 771-783, 1993.
20. Levine, S. J., P. Larivee, C. Logun, C. W. Angus, F. P. Ognibene, and J. H. Shelhamer. Tumor necrosis factor-α induces mucin hypersecretion and MUC-2 gene expression by human airway epithelial cells. Am. J. Respir. Cell Mol. Biol. 12: 196-204, 1995.
21. McCool, D. J., J. F. Forstner, and G. G. Forstner. Regulated and unregulated pathways for MUC2 mucin secretion in human colonie LS180 adenocarcinoma cells are distinct. Biochem. J. 312: 125-133, 1995.
22. McCool, D. J., J. F. Forstner, and G. G. Forstner. Synthesis and secretion of mucin by the human colonie tumour cell line LS180. Biochem. J. 302: 111-118, 1994.
23. Moniaux, N., S. Nollet, N. Porchet, P. Degand, A. Laine, and J. P. Aubert. Complete sequence of the human mucin MUC4: a putative cell membrane-associated mucin. Biochem. J. 338: 325-333, 1999.
24. Newton, A. C. Protein kinase C: structure, function, and regulation. J. Biol. Chem. 270: 28495-28498, 1995.
25. Olah, Z., C. Lehel, G. Jakab, and W. B. Anderson. A cloning and e-epitope-tagging insert for the expression of polymerase chain reaction-generated cDNA fragments in Escherichia coli and mammalian cells. Anal. Biochem. 221: 94-102, 1994.
26. Petrovics, G., C. Lehel, T. Oravecz, and W. B. Anderson. Protein kinase ∈-mediated growth inhibition of NIH 373 cells. Proc. Am. Assoc. Cancer Res. 39: 339, 1998.
27. Pigny, P., V. Guyonnet-Duperat, A. S. Hill, W. S. Pratt, S. Galiegue-Zouitina, M. D'Hooge, J.-P. Laine, I. Van-Seuningen, P. Degand, J. R. Gum, Y. S. Kim, D. M. Swallow, J.-P. Aubert, and N. Porchet. Human mucin genes assigned to 11p15. 5: identification and organization of a cluster of genes. Genomics: 340-352, 1996.
28. Porchet, N., P. Pigny, M.-P. Buisine, V. Debailleul, P. Degand, A. Laine, and J.-P. Aubert. Human mucin genes: genomic organization and expression of MUC4, MUC5AC and MUC5B. Biochem. Soc. Trans. 23: 800-805, 1995.
29. Shaoul, R., P. Marcon, Y. Okada, E. Cutz, and G. Forstner. Colonic expression of MUC2 and MUC5AC in inflammatory bowel disease (Abstract). J. Pediatr. Gastroenterol. Nutr. 26: 565, 1998.
30. Shapira, L., V. L. Sylvia, A. Halabi, W. A. Soskolne, T. E. Van Dyke, D. D. Dean, B. D. Boyan, and Z. Schwartz. Bacterial lipopolysaccharide induces early and late activation of protein kinase C in inflammatory macrophages by selective activation of PKC-∈. Biochem. Biophys. Res. Commun. 240: 629-634, 1997.
31. Toribara, N. W., A. M. Robertson, S. B. Ho, W. Kuo, E. Gum, J. W. Hicks, J. R. Gum, J. C. Byrd, B. Siddiki, and Y. S. Kim. Human gastric mucin. J. Biol. Chem. 268: 5879-5885, 1993.
32. Towbin, H., T. Staehelin, and J. Gordon. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and applications. Proc. Natl. Acad. Sci. USA 76: 4350-4354, 1979.
33. Tso, J. Y., X.-H. Sun, T.-H. Kao, K. S. Reece, and R. Wu. Isolation and character of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. Nucleic Acids Res. 13: 2485-2502, 1985.
34. Tytgat, K. M., F. J. Opdam, A. W. Einerhand, and J. Dekker. MUC2 is the prominent colonic mucin expressed in ulcerative colitis. Gut 38: 554-563, 1996.
35. Van Klinken, B. J., J. Dekker, H. A. Buller, C. De Bolos, and A. W. C. Einerhand. Biosynthesis of mucins (MUC2-6) along the longitudinal axis of the human gastrointestinal tract. Am. J. Physiol. 273 (Gastrointest. Liver Physiol. 36): G296-G302, 1997.
36. Van Klinken, B. J., E. Oussoren, J.-J. Weenink, G. J. Strous, H. A. Buller, J. Dekker, and A. W. C. Einerhand. The human intestinal cell lines Caco-2 and LS174T as models to study cell-type specific mucin expression. Glycoconj. J. 13: 757-768, 1996.
37. Velcich, A., and L. H. Augenlicht. Regulated expression of an intestinal mucin gene in HT29 colonic carcinoma cells. J. Biol. Chem. 268: 13956-13961, 1993.
38. Voynow, J. A., and M. C. Rose. Quantitation of mucin RNA in respiratory and intestinal epithelial cells. Am. J. Respir. Cell Mol. Biol. 11: 742-750, 1994.
39. Wyatt, T., H. Ito, T. S. Veys, and J. R. Spurzem. Stimulation of protein kinase C activity by tumor necrosis factor-α in bovine bronchial epithelial cells. Am. J. Physiol. 273 (Lung Cell. Mol. Physiol. 17): L1007-L1012, 1997.
40. Xu, G., L. Huan, I. Khatri, U. S. Sajjan, D. McCool, D. Wang, C. Jones, G. Forstner, and J. Forstner. Human intestinal mucin-like protein (MLP) is homologous with rat MLP in the C-terminal region and is encoded by a gene on chromosome 11 p 15.5. Biochem. Biophys. Res. Commun. 183: 821-828, 1992.