Chemical protein analysis: A comparison of Kjeldahl crude protein and total ninhydrin protein from wild, tropical vegetation

Journal of Chemical Ecology

Volumn 25, Issue 12, 1999, Pages 2601-2622

  Conklin Brittain, N L ^a   Dierenfeld, E S ^b   Wrangham, R W ^a   Norconk, M ^c   Silver, S C ^d  

^a HARVARD UNIVERSITY   (United States)

^b WILDLIFE CONSERVATION SOCIETY   (United States)

^c KENT STATE UNIVERSITY   (United States)

^d FORDHAM UNIVERSITY   (United States)

Author keywords

Amino acid analysis; Nitrogen protein conversion; Plant protein content; Total nitrogen

Indexed keywords

NINHYDRIN; NITROGEN; PROANTHOCYANIDIN; TANNIN; VEGETABLE PROTEIN;

MEASUREMENT METHOD; PHYTOCHEMISTRY; PROTEIN;

AMINO ACID ANALYSIS; ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; ECOLOGY; INTERMETHOD COMPARISON; NONHUMAN; PROTEIN ANALYSIS; PROTEIN CONTENT; QUANTITATIVE ASSAY; TROPICS; UGANDA; VEGETATION; WILDLIFE;

ANIMALIA;

EID: 0032739421     PISSN: 00980331     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1020835120701     Document Type: Article

References (42)

1. AOAC. 1984. Official Methods of Analysis of the Association of Official Analytical Chemists. S. Williams (ed.). Association of Official Analytical Chemists, Arlington, Virginia.
2. AMORY, A. M., and SCHUBERT, C. L. 1987. A method to determine tannin concentration by the measurement and quantification of protein-tannin interactions. Oecologia 73:420-424.
3. AURAND, L. W., WOODS, A. E., and WELLS, M. R. 1987. Food Composition and Analysis. Van Nostrand Reinhold, New York.
4. BARBEHENN, R. V. 1995. Measurement of protein in whole plant samples with ninhydrin. J. Sci. Food Agric. 69:353-359.
5. BATE-SMITH, E. C. 1975. Phytochemistry of proanthocyanidins. Phytochemistry 14:1107-1113.
6. BENSADOUN, A., and WEINSTEIN, D. 1976. Assay of proteins in the presence of interfering materials. Anal. Biochem. 70:241-250.
7. COMPTON, S. J., and JONES, C. G. 1985. Mechanism of dye response and interference in the Bradford Protein assay. Anal. Biochem. 151:369-374.
8. CONKLIN-BRITTAIN, N. L., WRANGHAM, R. W., and HUNT, K. D. 1998. Dietary response of chimpanzees and cercopithecines to seasonal variation in fruit abundance. II. Macronutrients. Int. J. Primatol 19:971-998.
9. DIERENFELD, E. S., DU TOIT, R., and BRASELTON, W. E. 1995. Nutrient composition of selected browses consumed by black rhinoceros (Diceros bicornis) in Zimbabwe. J. Zoo Wildl. Med. 26:220-230.
10. DIERENFELD, E. S., WILDMAN, R. E. C., and ROMO, S. Feed intake, diet utilization and composition of browses consumed by the Sumatran Rhino (Dicerorhinus sumatrensis) in a North American Zoo. Zoo Biol. Submitted.
11. DINTZIS, F. R., CAVINS, J. F., GRAF, E., and STAHLY, T. 1988. Nitrogen-to-protein conversion factors in animal feed and fecal samples. J. Anim. Sci. 66:5-11.
12. GRAFFAM, W., DIERENFELD, E. S., PATTILLO, G., and BASS, L. 1997. Evaluation of 8 species of native Texas browses as suitable forage substitutes for black rhinoceros (Diceros bicornis). Proceedings, Nutrition Advisory Group Conference, Fort Worth Zoo, Fort Worth, Texas.
13. GOERING, H. K., and VAN SOEST, P. J. 1970. Forage fiber analysis. Agricultural Handbook No. 379. A.R.S., U.S.D.A., Washington, D.C.
14. HANDLEY, L. L., MEHRAN, M., MOORE, C. A., and COOPER, W. J. 1989. Nitrogen-to-protein conversion factors for two tropical C4 grasses, Brachiaria mutica (Forsk) Stapf and Pennisetum purpureum Schumach. Biotropica 21:88-90.
15. HAGERMAN, A. E. 1987. Radial diffusion method for determining tannin in plant extracts. J. Chem. Ecol. 13:437-449.
16. HAGERMAN, A., and BUTLER, L. G. 1980. Condensed tannin purification and characterization of tannin-associated proteins. J. Agric. Food Chem. 28:947-952.
17. HAGERMAN, A., and BUTLER, L. G. 1981. The specificity of proanthocyanidin-protein interactions. J. Biol. Chem. 256:4494-4497.
18. HERBST, L. H. 1986. The role of nitrogen from fruit pulp in the nutrition of the frugivorous bat Carollia perspicillata. Biotropica 18:39-44.
19. IZHAKI, I. 1992. A comparative analysis of the nutritional quality of mixed and exclusive fruit diets for yellow-vented bulbuls. Condor 94:912-923.
20. IZHAKI, I. 1993. Influence of nonprotein nitrogen on estimation of protein from total nitrogen in fleshy fruits. J. Chem. Ecol. 19:2605-2515.
21. JONES, D. B. 1931. Factors for converting percentages of nitrogen in foods and feeds into percentages of proteins. Circular No. 183. United States Department of Agriculture, Washington, D.C.
22. JONES, C. G., HARE, J. D., and COMPTON, S. J. 1989. Measuring plant protein with the Bradford Assay 1. Evaluation and standard method. J. Chem. Ecol. 15:979-992.
23. KRISHNAMOORTHY, U., MUSCATO, T. V., SNIFFEN, C. F., and VAN SOEST, P. J. 1982. Nitrogen fractions in selected feedstuffs. J. Dairy Sci. 65:217-225.
24. LOWRY, O. H., ROSEBROUCH, N. J., FARR, A. R., and RANDALL, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
25. MAKKAR, H. P. S., DAWRA, R. K., and SINGH, B. 1988. Determination of both tannin and protein in a tannin-protein complex. J. Agric. Food Chem. 36:523-525.
26. MARKS, D. L., BUCHSBAUM, R., and SWAIN, T. 1985. Measurement of total protein in plant samples in the presence of tannins. Anal. Biochem. 147:136-143.
27. MARTIN, M. M., ROCKHOLM, D. C., and MARTIN, J. S. 1985. Effects of surfactants, pH, and certain cations on precipitation of proteins by tannins. J. Chem. Ecol. 11:485-494.
28. MARTINEZ DEL RIO, C. 1994. Nutritional ecology of fruit-eating and flower-visiting birds and bats, pp. 103-127, in D. J. Chivers, and P. Langer (eds.). The Digestive System in Mammals: Food, Form and Function. Cambridge University Press, Cambridge.
29. MILTON, K., and DINTZIS, F. R. 1981. Nitrogen-to-protein conversion factors for tropical plant samples. Biotropica 13:177-181.
30. NIJBOER, J., and DIERENFELD, E. S. 1996. Comparison of diets fed to Southeast Asian colobines in North American and European zoos, with emphasis on temperate browse composition. Zoo Biol. 15:499-508.
31. OFTEDAL, O. 1998. Do the physiologic responses of plants alter their nutritive value? It makes a difference for the desert tortoise. Proceedings, Comparative Nutrition Society, No. 2. Banff, Alberta, Canada, pp. 163-167.
32. PICHARD, G., and VAN SOEST, P. J. 1977. Protein solubility of ruminant feeds. Proceedings, Cornell Nutrition Conference for Feed Manufactures. Cornell University, Ithaca, New York, pp. 91-98.
33. PIERCE, W. C., and HAENISCH, E. L. 1948. Quantitative Analysis, 3rd ed. John Wiley & Sons, New York.
34. PORTER, L. J., HRSTICH, L. N., and CHAN, B. G. 1986. The conversion of procyanidins and prodel-phinidins to cyanidin and delphinidin. Phytochemistry 25:223-230.
35. ROBERTSON, J. B., and VAN SOEST, P. J. 1980. The detergent system of analysis and its application to human foods, pp. 123-158, in W. P. T. James and O. Theander (eds.). The Analysis of Dietary Fiber in Food. Marcel Dekker, New York.
36. SILVER, S. C. 1997. The feeding ecology of translocated howler monkeys, Alouatta pigra, in Belize. PhD dissertation. Fordham University, Bronx, New York.
37. SWAIN, T. 1979. Tannins and lignin, pp. 657-682, in G. A. Rosenthal and D. H. Janzen (eds.). Herbivores: Their Interaction with Secondary Plant Metabolites. Academic Press, New York.
38. VAN SOEST, P. J. 1994. Nutritional Ecology of the Ruminant, 2nd ed. Cornell University Press, Ithaca, New York.
39. WATT, B. K., and MERRILL, A. L. 1963. Composition of foods: Raw, processed, prepared. Agricultural Handbook No. 8. United States Department of Agriculture, Washington, D.C.
40. WILLIAMS, A. P. 1982. Determination of amino acids and peptides, pp. 285-311, in R. Macrae (ed.). HPLC in Food Analysis. Academic Press, London.
41. YEAGER, C. P., SILVER, S. C., and DIERENFELD, E. S. 1997. Mineral and phytochemical influences on foliage selection by the proboscis monkey (Nasalis larvatus). Am. J. Primatol. 41:117-128.
42. YEOH, H.-H., and WEE, Y.-C. 1994. Leaf protein contents and nitrogen-to-protein conversion factors for 90 plant species. Food Chem. 49:245-250.