Validation of a gastrointestinal explant system for measurement of mucosal antibody production

Clinical and Diagnostic Laboratory Immunology

Volumn 6, Issue 6, 1999, Pages 803-807

  Losonsky, Genevieve A ^a,c   Fantry, George T ^b   Reymann, Mardi ^a   Lim, Yu ^a  

^a Division of Emergency Medicine   (United States)

^b UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^c University of Maryland School of Medicine Center for Vaccine Development   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLOHEXIMIDE; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G;

ADULT; ANALYTIC METHOD; ANTIBODY PRODUCING CELL; ANTIBODY PRODUCTION; ANTIBODY TITER; ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DUODENUM BIOPSY; EXPLANT; FEMALE; HUMAN; HUMAN TISSUE; INCUBATION TIME; INTERMETHOD COMPARISON; LAMINA PROPRIA; MALE; MUCOSAL IMMUNITY; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS INHIBITION; STOMACH BIOPSY; SUPERNATANT; VALIDATION PROCESS;

EID: 0032734180     PISSN: 1071412X     EISSN: None     Source Type: Journal    
DOI: 10.1128/cdli.6.6.803-807.1999     Document Type: Article

References (17)

1. Brandzaeg, P., K. Kett, T. O. Rognum, J. Soderstrom, J. Bjorkander, T. Soderstrom, B. Petrusson, and L. A. Hanson. 1986. Distribution of mucosal IgA and IgG subclass-producing immunocytes and alterations in various disorders. Monogr. Allergy 20:179-201.
2. Cauci, S., R. Monte, S. Driussi, P. Lanzafame, and F. Quadrifoglio. 1998. Impairment of the mucosal immune system: IgA and IgM cleavage detected in vaginal washings of a subgroup of patients with bacterial vaginosis. J. Infect. Dis. 178:1698-1706.
3. Ferro, L. M., H. M. Weedon, L. R. Flego, D. Beroukas, and H. Zola. 1993. An organ fragment culture model to study lymphocyte activation in human lymphoid tissue. Immunohiology 188:51-61.
4. Kagnoff, M. F., R. M. Donaldson, and J. S. Trier. 1972. Organ culture of rabbit small intestine: prolonged in vitro steady state protein synthesis and secretion and secretory IgA secretion. Gastroenterology 63:541-551.
5. Kantele, A., and P. H. Makela. 1991. Different profiles of the human immune response to primary and secondary immunization with an oral Salmonella typhi Ty21a vaccine. Vaccine 9:423-425.
6. Kilian, M., J. Mestecky, and M. W. Russell. 1988. Defense mechanisms involving Fe-dependent functions of immunoglobulin A and their subversion by bacterial immunoglobulin A proteases. Microbiol. Rev. 52:296-303.
7. Logan, A. C., K.-P. N. Chow, A. George, P. D. Weinstein, and J. J. Cebra. 1991. Use of Peyer's patch and lymph node fragment cultures to compare local immune responses to Morganella inorganii. Infect. Immun. 59:1024-1031.
8. Losonsky, G. A., C. O. Tacket, S. S. Wasserman, J. B. Kaper, and M. M. Levine. 1993. Secondary Vibrio cholerae-specific cellular antibody responses following wild-type homologous challenge in people vaccinated with CVD 103-HgR live oral cholera vaccine: changes with time and lack of correlation with protection. Infect. Immun. 61:729-733.
9. Lycke, N., A. K. Bromander, and J. Holmgren. 1989. Role of local IgA antitoxin-producing cells for intestinal protection against cholera toxin challenge. Int. Arch. Allergy Appl. Immunol. 88:273-279.
10. Lycke, N., A. Kilander, L. A. Nilsson, A. Tarkowski, and N. Werner. 1989. Production of antibodies to gliadin in intestinal mucosa of patients with coeliac disease: a study at the single cell level. Gut 30:72-77.
11. Mestecky, J., C. Lue, and M. W. Russell. 1991. Selective transport of IgA: cellular and molecular aspects. Gastroenterol. Clin. N. Am. 20:441-471.
12. Moldoveanu, Z., M. L. Egan, and J. Mestecky. 1984. Cellular origins of human polymeric and monomeric IgA: intracellular and secreted forms of IgA. J. Immunol. 133:3156-3160.
13. Quiding, M., I. Nordstrom, A. Kilander, G. Andersson, L. A. Hanson, J. Holmgren, and C. Czerkinsky. 1991. Oral cholera vaccination induces strong intestinal antibody responses and interferon-γ production and evokes local immunoglobulin memory. J. Clin. Investig. 88:143-148.
14. Quiding-Järbrink, M., G. Granström, I. Nordström, J. Holmgren, and C. Czerkinsky. 1995. Induction of compartmentalized B-cell responses in human tonsils. Infect. Immun. 63:853-857.
15. Schrader, C. E., R. L. Kerlin, A. George, and J. J. Cebra. 1990. Dendritic cells support the production of IgA and other non-IgM isotypes in clonal culture. Int. Immunol. 2:563-570.
16. Trier, J. S. 1976. Organ culture methods in the study of gastrointestinal mucosal function and development. N. Engl. J. Med. 295:150-155.
17. Wilson, N. W., and J. C. McCartney. 1986. Organ culture of human gastric mucosa and gastric cancers. J. Pathol. 150:127-134.