Isolation and transformation of uracil auxotrophs of the edible basidiomycete Pleurotus ostreatus

FEMS Microbiology Letters

Volumn 181, Issue 2, 1999, Pages 225-228

  Kim, Beom Gi ^a   Magae, Yumi ^b   Yoo, Young Bok ^a   Kwon, Suk Tae ^c  

^a National Institute of Animal Science   (South Korea)

^b FORESTRY AND FOREST PRODUCTS RESEARCH INSTITUTE   (Japan)

^c Sungkyunkwan University   (South Korea)

Author keywords

Monophosphate decarboxylase; Orotidine; Pleurotus ostreatus; Transformation; Uracil auxotrophic marker

Indexed keywords

DNA MARKER; FLUOROOROTIC ACID; OROTIDINE 5' PHOSPHATE DECARBOXYLASE; URACIL;

ARTICLE; AUXOTROPHIC MUTANT; BASIDIOMYCETES; CELL TRANSFORMATION; DRUG METABOLISM; DRUG RESISTANCE; GENE TRANSFER; PLASMID; PRIORITY JOURNAL; SOUTHERN BLOTTING;

ASPARTATE CARBAMOYLTRANSFERASE; BLOTTING, SOUTHERN; CARBAMOYL-PHOSPHATE SYNTHASE (GLUTAMINE-HYDROLYZING); DNA, FUNGAL; DRUG RESISTANCE, MICROBIAL; FUNGAL PROTEINS; GENETIC VECTORS; MULTIENZYME COMPLEXES; MUTATION; OROTIC ACID; PLASMIDS; PLEUROTUS; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSFORMATION, GENETIC; TRICHODERMA; ULTRAVIOLET RAYS; URACIL;

EID: 0032734083     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00543-1     Document Type: Article

References (12)

1. Chang, S.-T and Miles, P.G. (1989) Edible Mushrooms and Their Cultivation, pp. 165-166. CRC Press Inc.
2. Peng M., Singh N.L., Lemke P.A. Recovery of recombinant plasmids from Pleurotus ostreatus transformants. Curr. Genet. 22:1992;53-59.
3. Yanai K., Yonekura K., Usami H., Hirayama M., Kajiwara S., Yamazaki T., Shishido K., Adachi T. The integrative transformation of Pleurotus ostreatus using bialaphos resistance as a dominant selectable marker. Biosci. Biotechnol. Biochem. 60:1996;472-475.
4. Peng M., Lemke P.A., Singh N.K. A nucleotide sequence involved in replicative transformation of a filamentous fungi. Curr. Genet. 24:1993;114-121.
5. Boeke J.D., LaCroute F., Fink G.R. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5′-fluoro-orotic acid resistance. Mol. Gen. Genet. 197:1984;345-346.
6. Smith J.L., Bayliss F.T., Ward M. Sequence of the cloned pyr 4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformation. Curr. Genet. 19:1991;27-33.
7. Berges T., Barreau C. Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes. Curr. Genet. 19:1991;359-365.
8. Goosen T., Bloemheuvel G., Gysler C., deBie D.A., van den Broek H.W.J., Swart K. Transformation of Aspergillus niger using the homologous orotidine-5′-phosphate decarboxylase gene. Curr. Genet. 11:1987;499-503.
9. Kim B.-G., Park S.-C., Jeong M.-J., Yoo Y.B., Ryu J.-C., Kwon S.-T. A positive selection for pyrimidine auxotrophic mutants from basidiospores of Pleurotus sajor-caju using 5′-fluoro-orotic acid. Korean J. Mycol. 25:1997;26-29.
10. Raeder U., Broda P. Rapid preparation of DNA from filamentous fungi. Lett. Appl. Microbiol. 1:1985;17-20.
11. Akileswaran L., Alic M., Clark E.K., Hornick J.L., Gold M.H. Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium. Curr. Genet. 23:1993;351-356.
12. Noel T., Labarere J. Homologous transformation of the edible basidiomycete Agrocybe aegerita with URA1 gene: characterization of integrative events and of rearranged free plasmids in transformants. Curr. Genet. 25:1994;432-437.