Construct for high-level expression and low misincorporation of lysine for arginine during expression of pET-encoded eukaryotic proteins in Escherichia coli

BioTechniques

Volumn 27, Issue 5, 1999, Pages 950-954

  You, Jianxin ^a   Cohen, Robert E ^b   Pickart, Cecile M ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; CHLORAMPHENICOL; LYSINE; PROTEIN; UBIQUITIN;

ARTICLE; CODON; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENE SEQUENCE; GENE TARGETING; MOLECULAR CLONING; NONHUMAN; PLASMID;

ESCHERICHIA COLI; EUKARYOTA;

EID: 0032727419     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99275st01     Document Type: Article

References (18)

1. Arnason, T. and M.J. Ellison. 1994. Stress resistance in Saccharomyces cerevisiae is strongly correlated with assembly of a novel type of multiubiquitin chain. Mol. Cell. Biol. 14:7876-7883.
2. Beal, R., Q. Deveraux, G. Xia, M. Rechsteiner and C.M. Pickart. 1996. Surface hydrophobic residues of multiubiquitin chains essential for proteolytic targeting. Proc. Natl. Acad. Sci. USA 95:861-866.
3. Brinkmann, U., R.E. Mattes and P. Buckel. 1989. High-level expression of recombinant genes in Escherichia coli is dependent on the availability of the dnaY gene product. Gene 85:109-114.
4. Buhler, C., D. Gadelle, P. Forterre, J.C. Wang and A. Bergerat. 1998. Reconstitution of DNA topoiosomerase VI of the thermophilic archaeon Sulfolobus shibatae from subunits separately overexpressed in Escherichia coli. Nucleic Acids Res. 26:5157-5162.
5. Calderone, T.L., R.D. Stevens and T.G. Oas. 1996. High-level misincorporation of lysine for arginine at AGA codons in a fusion protein expressed in Escherichia coli. J. Mol. Biol. 262:407-412.
6. Chang, A.C. and S.N. Cohen. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134:1141-1156.
7. Chen, G.T. and M. Inouye. 1994. Role of the AGA/AGG codons, the rarest codons in global gene expression in Escherichia coli. Genes Dev. 8:2641-2652.
8. Chen, Z. and C.M. Pickart. 1990. A 25-kilodalton ubiquitin carrier protein (E2) catalyzes multi-ubiquitin chain synthesis via lysine 48 of ubiquitin. J. Biol. Chem. 265:21835-21842.
9. Forman, M.D., R.F. Stack, P.S. Masters, C.R. Hauer and S.M. Baxter. 1998. High level, context dependent misincorporation of lysine for arginine in Saccharomyces cerevisiae al homeodomain expressed in Escherichia coli. Protein Sci. 7:500-503.
10. Haas, A.L. and P.M. Bright 1985. The immunochemical detection and quantitation of intracellular ubiquitin-protein conjugates. J. Biol. Chem. 260:12464-12473.
11. Haldeman, M.T., G. Xia, E.M. Kasperek and C.M. Pickart. 1997. Structure and function of ubiquitin conjugating enzyme E2-25K: the tail is a core-dependent activity element. Biochemistry 36:10526-10537.
12. Hochstrasser, M. 1996. Ubiquitin-dependent protein degradation. Annu. Rev. Genet. 30:405-439.
13. Hodgins, R., C. Gwozd, T. Arnason, M. Cummings and M.J. Ellison. 1996. The tail of a ubiquitin-conjugating enzyme redirects multi-ubiquitin chain synthesis from the lysine 48-linked configuration to a novel nonlysine-linked form. J. Biol. Chem. 277:28766-28771.
14. Pickart, C.M. 1997. Targeting of substrates to the 26S proteasome. FASEB J. 11:1055-1066.
15. Saxena, P. and J.R. Walker. 1992. Expression of argU, the Escherichia coli gene coding for a rare arginine tRNA. J. Bacteriol. 174:1956-1964.
16. ArgtRNAs. BioTechniques 19:196-200.
17. Spanjaard, R.A., K. Chen, J.R. Walker and J. van Duin. 1990. Frameshift suppression at tandem AGA and AGG codons by cloned tRNA genes: assigning a codon to argU tRNA and T4 tRNA(Arg). Nucleic Acids Res. 18:5031-5036.
18. Studier, F.W. 1991. Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. J. Mol. Biol. 219:37-44.