Determination of the activity of the cytochrome p450 enzyme geraniol 10-hydroxylase in plants by high-performance liquid chromatography

Phytochemical Analysis

Volumn 10, Issue 6, 1999, Pages 314-318

  Collu, Graziella ^a   Bink, Hugo H J ^a   Moreno, Paulo R H ^a   Van Der Heijden, Robert ^a   Verpoorte, Robert ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Catharanthus roseus; Cytochrome P450; Geraniol 10 hydroxylase; High performance liquid chromatography; Terpenoid biosynthesis

Indexed keywords

BIOCHEMISTRY; CELL CULTURE; ENZYMES; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MONOTERPENES;

CATHARANTHUS ROSEUS; CYTOCHROME P450 ENZYMES; CYTOCHROMES P450; GERANIOL 10-HYDROXYLASE; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLASES; REVERSED PHASE; TERPENOID BIOSYNTHESIS; TERPENOIDS; UV DETECTION;

BIOSYNTHESIS;

CYTOCHROME P450; GERANIOL; NEROL; OXYGENASE;

ARTICLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PHYTOCHEMISTRY;

CATHARANTHUS ROSEUS;

EID: 0032727267     PISSN: 09580344     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1099-1565(199911/12)10:6<314::AID-PCA475>3.0.CO;2-Z     Document Type: Article

References (15)

1. Battersby, A. R., Brown, S. H. and Payne, T. G. (1970). Biosynthesis of loganin and the indole alkaloids from hydroxygeraniol-hydroxynerol. Chem. Comm. 740, 827-828.
2. Berlin, J., Forche, E., Wray, V., Hammer, J. and Hösel, W. (1983). Formation of benzophenanthridine alkaloids by suspension cultures of Eschscholtzia californica. Z. Naturforsch. 38c, 346-352.
3. Hallahan, D. L., Nugent, J. H. A., Hallahan, B. J., Dawson, G. W., Smiley, D. W. M., West J. M. and Wallsgrove, R. M. (1992). Interactions of avocado (Persea americana) cytochrome P450 with monoterpenoids. Plant Physiol. 98, 1290-1297.
4. Hallahan, D. L., Lau, S. M. C., Harder, P. A., Smiley, D. W. M., Dawson, G. W., Pickett, J. A., Christoffersen, R. E. and O'Keefe, D. P. (1994). Cytochrome P450-catalysed monoterpenoid oxidation in catmint (Nepeta racemosa) and avocado (Persea americana); evidence for related enzymes with different activities. Biochim. Biophys. Acta 1201, 94-100.
5. Kubeczka, K. H. (1985). Progress in isolation techniques for essential oil constituents. In Essential oils and Aromatic Plants (Baerheim Svendsen, A. and Scheffer, J. J. C., eds.). pp. 107-126. Martinus Nijhoff/Junk, Dordrecht.
6. Madyastha, K. M., Meehan, T. D. and Coscia, C. J. (1976). Characterisation of a cytochrome P450 dependent monoterpene hydroxylase from the higher plant Vinca rosea. Biochemistry 15, 1097-1102.
7. McFarlane, J., Madyastha, K. M. and Coscia, C. J. (1975). Regulation of secondary metabolism in higher plants. Effect of alkaloids on a cytochrome P450 dependent monooxygenase. Biochem. Biophys. Res. Commun. 66, 1263-1269.
8. Meehan, T. D. and Coscia, C. J. (1973). Hydroxylation of geraniol and nerol by a monooxygenase from Vinca rosea. Biochem. Biophys. Res. Commun. 53, 1043-1048.
9. Meijer, A. H., de Waal, A and Verpoorte, R. (1993). Purification of the cytochrome P450 enzyme geraniol 10-hydroxylase from cell cultures of Catharanthus roseus. J. Chromatogr. 635, 237-249.
10. Moreno, P. R. H., van der Heijden, R. and Verpoorte, R. (1993). Effect of terpenoid precursor feeding and elicitation on formation of indole alkaloids in cell suspension cultures of Catharanthus roseus. Plant Cell Rep. 12, 702-705.
11. Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15, 473-497.
12. Peterson, G. L. (1977). A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83, 346-357.
13. Schiel, O., Witte, L. and Berlin, J. (1987). Geraniol 10-hydroxylase activity and its relation to monoterpene indole alkaloid accumulation in cell suspension cultures of Catharanthus roseus. Z. Naturforsch. 42c, 1075-1081.
14. Stafford, A. and Smith, L. (1986). Effects of modification of the primary precursor level by selection and feeding on indole alkaloid accumulation in Catharanthus roseus. In Secondary Metabolism in Plant Cell Cultures (Morris, P., Scragg, A. H., Stafford, A. and Fowler, M. W., eds.). pp. 250-256. Cambridge University Press, Cambridge.
15. Verpoorte, R., van der Heijden, R. and Moreno, P. R. H. (1997). Biosynthesis of terpenoid indole alkaloids in Catharanthus roseus cells. In The Alkaloids (Cordell, G. A., ed.) Vol 49, pp. 221-229. Academic Press, New York.