Characterization of the regulatory region of the human testis-specific form of the pyruvate dehydrogenase α-subunit (PDHA-2) gene

Biochimica et Biophysica Acta - Gene Structure and Expression

Volumn 1447, Issue 2-3, 1999, Pages 236-243

  Datta, Utpal ^a   Wexler, Isaiah D ^b,c   Kerr, Douglas S ^b   Raz, Itamar ^c   Patel, Mulchand S ^a  

^a UNIVERSITY AT BUFFALO   (United States)

^b CASE WESTERN RESERVE UNIVERSITY   (United States)

^c HADASSAH HEBREW UNIVERSITY MEDICAL CENTER   (Israel)

Author keywords

Gene regulation; Human pyruvate dehydrogenase subunit gene (PDHA 2); Promoter sequence

Indexed keywords

PYRUVATE DEHYDROGENASE;

ARTICLE; CHROMOSOME 4; CONTROLLED STUDY; DNA FOOTPRINTING; ENHANCER REGION; GENE CONTROL; GENE EXPRESSION; HUMAN; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; REPRESSOR GENE; SPERMATOCYTE; TRANSCRIPTION INITIATION; X CHROMOSOME;

BASE SEQUENCE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; MALE; MOLECULAR SEQUENCE DATA; ORGAN SPECIFICITY; PROMOTER REGIONS (GENETICS); PYRUVATE DEHYDROGENASE (LIPOAMIDE); PYRUVATE DEHYDROGENASE COMPLEX; TESTIS;

EID: 0032724850     PISSN: 01674781     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4781(99)00158-X     Document Type: Article

References (24)

1. 1α subunit of the human pyruvate dehydrogenase complex. Genomics. 4:1989;174-181.
2. 1 alpha subunit is coded for by an intronless gene on chromosome 4. Genomics. 8:1990;225-232.
3. 1 alpha subunit genes in the mouse: mapping and comparison with human homologs. Somat. Cell Mol. Genet. 16:1990;487-492.
4. 1 alpha genes. Biochim. Biophys. Acta. 1131:1992;83-90.
5. 1α subunit genes during spermatogenesis in adult mouse. Exp. Cell Res. 199:1992;39-49.
6. 1 alpha subunit. Biol. Reprod. 47:1992;48-58.
7. Fitzgerald J., Dahl H.H.M., Iannello R.C. Differential expression of two testis-specific transcripts of the mouse Pdha-2 gene during spermatogenesis. DNA Cell Biol. 13:1994;531-537.
8. Young J.C., Gould J.A., Kola I., Iannello R.C. Review: Pdha-2, past and present. J. Exp. Zool. 282:1998;231-238.
9. 1α gene. Hum. Mutat. 1:1992;97-102.
10. Fitzgerald J., Wilcox S.A., Graves J.A., Dahl H.H. A eutherian X-linked gene, PDHA1, is autosomal in marsupials: a model for the evolution of a second, testis-specific variant in eutherian mammals. Genomics. 18:1993;636-642.
11. Graves J.A., Watson J.M. Mammalian sex chromosomes: evolution of organization and function. Chromosoma. 101:1991;63-68.
12. Frohman M.A., Dush M.K., Martin G.R. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA. 85:1988;8998-9002.
13. Edwards A.D., Delort J., Mallet J. Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries by in vitro amplification. Nucleic Acids Res. 19:1991;5227-5232.
14. Iannello R.C., Kola I., Dahl H.H.M. Temporal and tissue-specific interactions involving novel transcription factors and the proximal promoter of the mouse Pdha-2 gene. J. Biol. Chem. 268:1993;22581-22590.
15. Wigler M., Sweet R., Sim G.K., Wold B., Pellicer A., Lacy E., Maniatis T., Silverstein S., Axel R. Transformation of mammalian cells with genes from procaryotes and eucaryotes. Cell. 16:1979;777-785.
16. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual, 2nd edn., Cold Spring Harbor Press, New York, 1989.
17. Iannello R.C., Young J., Sumarsono S., Tymms M.J., Dahl H.H., Gould J., Hedger M., Kola I. Regulation of Pdha-2 expression is mediated by proximal promoter sequences and CpG methylation. Mol. Cell Biol. 17:1997;612-619.
18. Saiki R.K., Gelfand D.H., Stoffel S., Scharf S.J., Higuchi R., Horn G.T., Mullis K.B., Erlich H.A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 239:1988;487-491.
19. Gorski K., Carneiro M., Schibler U. Tissue-specific in vitro transcription from the mouse albumin promoter. Cell. 47:1986;767-776.
20. Thiesen H.J., Bach C. Target Detection Assay (TDA): a versatile procedure to determine DNA binding sites as demonstrated on SP1 protein. Nucleic Acids Res. 18:1990;3203-3209.
21. Goto M., Masamune Y., Nakanishi Y. A factor stimulating transcription of the testis-specific Pgk-2 gene recognizes a sequence similar to the binding site for a transcription inhibitor of the somatic-type Pgk-1 gene. Nucleic Acids Res. 21:1993;209-214.
22. Iannello R.C., Young J.C., Sumarsono S., Tymms M.J., Kola I. Mouse testis Pdha-2 promoter upstream sequences confer tissue- and temporal-specific activity in transgenic mice. Reprod. Fertil. Dev. 6:1994;599-604.
23. Brown T.A., McKnight S.L. Specificities of protein-protein and protein-DNA interaction of GABP alpha and two newly defined ets-related proteins. Genes Dev. 6:1992;2502-2512.
24. McCarrey J.R. Nucleotide sequence of the promoter region of a tissue-specific human retroposon: comparison with its housekeeping progenitor. Gene. 61:1987;291-298.