Comparison of PLRV and PVY detection in potato seed samples tested by Florida winter field inspection and RT-PCR

American Journal of Potato Research

Volumn 76, Issue 5, 1999, Pages 313-316

  Russo, P ^a   Miller, L ^a   Singh, R P ^b   Slack, S A ^a  

^a Department of Obstetrics Gynecology   (United States)

^b AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

Dormant tubers; RT PCR; Virus detection

Indexed keywords

PLANT MICROORGANISM INTERACTION; POTATO; POTATO LEAFROLL LUTEOVIRUS; POTATO POTYVIRUS Y; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; UNITED STATES; VIRUS DETECTION;

ANIMALIA; LUTEOVIRUS; POTATO LEAFROLL VIRUS; POTATO VIRUS Y; POTYVIRUS; SOLANUM TUBEROSUM;

EID: 0032722836     PISSN: 00030589     EISSN: None     Source Type: Journal    
DOI: 10.1007/bf02853630     Document Type: Article

References (6)

1. Henson, J.M. and R. French. 1993. The polymerase chain reaction and plant disease diagnosis. Ann Rev Phytopathology 31:81-100.
2. Sambrook, J., E.F. Fritsch, and T. Maniatis 1989. Gel Electrophoresis of DNA. In: Molecular cloning; a laboratory manual, C. Nolan (Ed.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. Chapter 6, 6.7-6.19.
3. Singh, R.P., J. Kurz, and G. Boiteau. 1996, Detection of style-borne and circulative potato viruses in aphids by duplex reverse transcription polymerase chain reaction. J Virol Methods 59:189-196.
4. Slack, S.A. 1993. Seed certification and seed improvement programs. In: Potato Health Management, R.C. Rowe (Ed.). APS Press, St. Paul, MN. Chapter 7, 61-66.
5. Souza-Dias, J.A.C., P. Russo, J.A. Betti, L. Miller, and S.A. Slack. 1999a. Simplified extraction method for ELISA and PCR detection of potato leafroll luteovirus primary infection in dormant potato tubers. Amer J Potato Res (In press).
6. Souza-Dias, J.A.C., P. Russo, L. Miller, and S.A. Slack. 1999b. Comparison of nucleotide sequences from three potato leafroll virus (PLRV) isolates collected in Brazil. Amer J Potato Res 76:17-24.