Direct selection of IS903 transposon insertions by use of a broad-host-range vector: Isolation of catalase-deficient mutants of Actinobacillus actinomycetemcomitans

Journal of Bacteriology

Volumn 181, Issue 23, 1999, Pages 7298-7307

  Thomson, Valeri J ^a,b,e   Bhattacharjee, Mrinal K ^c   Fine, Daniel H ^d   Derbyshire, Keith M ^a,b   Figurski, David H ^c  

^a WADSWORTH CENTER   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

^c The New York Presbyterian Hospital   (United States)

^d UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY   (United States)

^e BARD COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALASE; HYDROGEN PEROXIDE; KANAMYCIN; NUCLEOTIDE; PYRANOSIDE; TRANSPOSASE;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; ARTICLE; BACTERIUM ISOLATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE INSERTION; HAEMOPHILUS INFLUENZAE; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROMOTER REGION; TRANSPOSON;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS;

EID: 0032720316     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.23.7298-7307.1999     Document Type: Article

References (49)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1989. Current protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y.
2. Berg, C. M., D. E. Berg, and E. A. Groisman. 1989. Transposable elements and the genetic engineering of bacteria, p. 879-925. In C. M. Berg and M. M. Howe (ed.), Mobile DNA. American Society for Microbiology, Washington, D.C.
3. Berg, C. M., and D. E. Berg. 1996. Transposable element tools for microbial genetics, p. 2588-2612. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
4. Bishai, W. R., H. O. Smith, and G. J. Barcak. 1994. A peroxide/ascorbate-inducible catalase from Haemophilus influenzae is homologous to the Escherichia coli katE gene product. J. Bacteriol. 176:2914-2921.
5. Brown, S. M., M. L. Howell, M. L. Vasil, A. J. Anderson, and D. J. Hassett. 1995. Cloning and characterization of the katB gene of Pseudomonas aeruginosa encoding a hydrogen peroxide-inducible catalase: purification of KatB, cellular localization, and demonstration that it is essential for optimal resistance to hydrogen peroxide. J. Bacteriol. 177:6536-6544.
6. Buzy, A., V. Bracchi, R. Sterjiades, J. Chroboczek, P. Thibault, J. Gagnon, H. Jouve, and G. Hudry-Clergeon. 1995. Complete amino acid sequence of Proteus mirabilis PR catalase. Occurrence of a methionine sulfone in the close proximity of the active site. J. Protein Chem. 14:59-72.
7. Derbyshire, K. M. 1995. An IS903-based vector for transposon mutagenesis and the isolation of gene fusions. Gene 165:143-144.
8. Derbyshire, K. M., and N. D. Grindley. 1996. cis preference of the IS903 transposase is mediated by a combination of transposase instability and inefficient translation. Mol. Microbiol. 21:1261-1272.
9. Derbyshire, K. M., L. Hwang, and N. D. Grindley. 1987. Genetic analysis of the interaction of the insertion sequence IS903 transposase with its terminal inverted repeats. Proc. Natl. Acad. Sci. USA 84:8049-8053.
10. Derbyshire, K. M., M. Kramer, and N. D. Grindley. 1990. Role of instability in the cis action of the insertion sequence IS903 transposase. Proc. Natl. Acad Sci. USA 85:4048-4052.
11. Dongari, A. I., and K. T. Miyasaki. 1991. Sensitivity of Actinobacillus actinomycetemcomitans and Haemophilus aphrophitus to oxidative killing. Oral Microbiol. Immunol. 6:363-372.
12. Fine, D., D. Furgang, H. Schreiner, P. Goncharoff, G. Charlesworth, G. Ghazwan, P. Fitzgerald-Bocarsly, and D. H. Figurski. 1999. Phenotypic variation in Actinobacillus actinomycetemcomitans during laboratory growth: implications for virulence. Microbiology 145:1335-1347.
13. Fives-Taylor, P., D. Meyer, K. Mintz, and C. Brissette. 1999. Virulence factors of Actinobacillus actinomycetemcomitans. Periodontol. 2000 20:136-167.
14. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J. F. Tomb, B. A. Dougherty, J. M. Merrick, K. McKenney, G. Sutton, W. FitzHugh, C. Fields, J. D. Gocayne, J. Scott, R. Shirley, L. Lu, A. Glodek, J. M. Kelley, J. F. Weideman, C. A. Phillips, T. Spriggs, E. Hedblom, M. D. Cotton, T. R. Utterback, M. C. Hanna, D. T. Nguyen, D. M. Saudek, R. C. Brandon, L. D. Fine, J. L. Fritchman, J. L. Fuhrmann, N. S. M. Geoghagen, C. L. Gnehrn, L. A. McDonald, K. V. Small, C. M. Fraser, H. O. Smith, and J. C. Venter. 1995. Whole-genome random sequencing and assembly of Haemophilia influenzae Rd. Science 269:496-512.
15. Friedberg, E., G. Walker, and W. Siede. 1995. DNA repair and mutagenesis. ASM Press, Washington, D.C.
16. Fürste, J., W. Pansegrau, R. Frank, H. Blöcker, P. Scholz, M. Bagdasarian, and E. Lanka. 1986. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48:119-131.
17. Goncharoff, P., J. K. K. Yip, H. Wang, H. C. Schreiner, J. A. Pai, D. Furgang, R. H. Stevens, D. H. Figurski, and D. H. Fine. 1993. Conjugal transfer of broad-host-range incompatibility group P and group Q plasmids from Escherichia coli to Actinobacillus actinomycetemcomitans. Infect. Immun. 61:3544-3547.
18. Grindley, N., and C. M. Joyce. 1981. Analysis of the structure and function of the kanamycin-resistance transposon Tn903. Cold Spring Harbor Symp. Quant. Biol. 45:125-133.
19. Guiney, D. 1993. Broad host-range conjugation and mobilizable plasmids in gram-negative bacteria, p. 75-103. In D. B. Clewell (ed.), Bacterial conjugation. Plenum Publishing Corp., New York, N.Y.
20. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
21. Hassan, H. M., and I. Fridovich. 1978. Regulation of the synthesis of catalase and peroxidase in Escherichia coli. J. Biol. Chem. 253:6445-6420.
22. Hu, W.-Y., and K. M. Derbyshire. 1998. Target choice and orientation preference of the insertion sequence IS903. J. Bacteriol. 180:3039-3048.
23. Johnson, S. R., B. M. Steiner, and G. H. Perkins. 1999. Cloning and characterization of the catalase gene of Neisseria gonorrhoeae: use of the gonococcus as a host organism for recombinant DNA. Infect. Immun. 64:2627-2634.
24. Kacblany, S. C., P. J. Planet, D. Fine, M. K. Bhattacharjee, E. Kollia, R. DeSalle, D. H. Fine, and D. H. Figurski. 1999. Unpublished data.
25. Kahn, M., R. Kolter, C. Thomas, D. H. Figurski, R. Meyer, E. Remaut, and D. R. Helinski. 1979. Plasmid cloning vehicles derived from plasmids ColE1, F, R6K, and RK2. Methods Enzymol. 68:268-280.
26. Kaplan, J., and D. Fine. 1998. Codon usage in Actinobacillus actinomycetemcomitans. FEMS Microbiol. Lett. 163:31-36.
27. Kilian, M. 1976. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J. Gen. Microbiol. 93:9-62.
28. Kleckner, N., J. Bender, and S. Gottesman. 1991. Uses of transposons with emphasis on Tn10. Methods Enzymol. 204:139-180.
29. Kolodrubetz, D., and E. Kraig. 1994. Transposon Tn5 mutagenesis of Actinobacillus actinomycetemcomitans via conjugation. Oral Microbiol. Immunol. 9:290-296.
30. Kornacki, J. 1999. Unpublished data.
31. Loewen, P. C., J. Switala, and B. L. Triggs-Raine. 1985. Catalans HPI and HPII in Escherichia coli are induced independently. Arch. Biochem. Biophys. 243:144-149.
32. Loewen, P. C., and B. L. Triggs. 1984. Genetic mapping of katF, a locus that with katE affects the synthesis of a second catalase species in Escherichia coli. J. Bacteriol. 160:668-675.
33. Marquis, R. E. 1995. Oxygen metabolism, oxidative stress and acid-base physiology of dental plaque biofilms. J. Ind. Microbiol. 15:198-207.
34. Miyasaki, K. T., M. E. Wilson, H. S. Reynolds, and R. J. Genco. 1984. Resistance of Actinobacillus actinomycetemcomitans and differential susceptibility of oral Haemophilus species to the bactericidal effects of hydrogen peroxide. Infect. Immun. 46:644-648.
35. Hoe, B., F. Najar, S. Clifton, T. Ducey, L. Lewis, and D. Dyer. October 1999, revision date. Actinobacillus Genome Sequencing Project. [Online.] http:// www.genome.ou.edu/act.html. [25 October 1999, last date accessed.]
36. Roos, M., and D. Roos. 1978. Differences in oxygen metabolism of phagocytosing monocytes and neutrophils. J. Clin. Investig. 61:480-488.
37. Rosan, B., J. Slots, R. J. Lament, M. A. Listgarten, and G. M. Nelson. 1988. Actinobacillus actinomycetemcomitans fimbriae. Oral Microbiol. Immunol. 3:58-63.
38. Sak, B. D., A. Eisenstark, and D. Touati. 1989. Exonuclease III and the catalase hydroperoxidase II in Escherichia coli are both regulated by the katF gene product. Proc. Natl. Acad. Sci. USA 86:3271-3275.
39. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
40. Sato, S., N. Takamatsu, N. Okahashi, N. Matsunoshita, I. Inoue, T. Takehara, and T. Koga. 1992. Construction of mutants of Actinobacillus actinomycetemcomitans defective in serotype b-specific polysaccharide antigen by insertion of transposon Tn916. J. Gen. Microbiol. 138:1203-1209. (Erratum, 138:1992.)
41. Schwartz, E., M. Kroger, and B. Rak. 1988. IS150: distribution, nucleotide sequence and phylogenetic relationships of a new E. coli insertion element. Nucleic Acids Res. 16:6789-6802.
42. Sia, E., D. M. Kuehner, and D. H. Figurski. 1996. The mechanism of retrotransfer in conjugation: prior transfer of the conjugative plasmid is required. J. Bacteriol. 178:1457-1464.
43. Slots, J. 1976. The predominant cultivable organism in juvenile petiodontitis. Scand. J. Dent. 84:1-10.
44. Smith, H. O., J. F. Tomb, B. A. Dougherty, R. D. Fleischmann, and J. C. Venter. 1995. Frequency and distribution of DNA uptake signal sequences in the Haemophilus influenzae Rd genome. Science 269:538-540.
45. Storz, G., L. A. Tartaglia, and B. N. Ames. 1990. Transcriptional regulator of oxidative stress-inducihle genes: direct activation by oxidation. Science 248: 189-194.
46. Tonjum, T., G. Bukholm, and K. Bovre. 1990. Identification of Haemophilus aphrophilus and Actinobacillus actinomycetemcomitans by DNA-DNA hybridization and genetic transformation. J. Clin. Microbiol. 28:1994-1998.
47. Triglia, T., M. Peterson, and D. Kemp. 1988. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res. 16:8186.
48. van Winkelhoff, A., and J. Slots. 1999. Actinobacillus actinomycetemcomitans and Prophorymonas gingivalis in non-oral infections. Periodontol. 2000 20: 122-135.
49. Water, L., A. B. Sadosky, and H. A. Shuman. 1994. Mutagenesis of Legionella pneumophila using Tn903d11/lacZ: identification of a growth-phase regulated pigmentation gene. Mol. Microbiol. 11:641-653.