Determination of colony numbers in pig epidermis as an estimate for radiosensitivity. A rapid assay based on in vitro BrdU-labelling

British Journal of Radiology

Volumn 72, Issue NOV., 1999, Pages 1104-1109

  Van Den Aardweg, G J M J ^a   Mooi, W J ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BROXURIDINE; DISPASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BIOASSAY; CELL DAMAGE; CELL LABELING; CELL PROLIFERATION; CELL SURVIVAL; CLONOGENESIS; COLONY FORMATION; DERMIS; DESQUAMATION; EPIDERMIS; EPIDERMIS CELL; FEMALE; IMMUNOHISTOCHEMISTRY; IRRADIATION; MATHEMATICAL ANALYSIS; NONHUMAN; RADIATION DOSE; RADIATION INJURY; RADIOSENSITIVITY; SKIN BIOPSY; SKINFOLD THICKNESS; SWINE;

EID: 0032711070     PISSN: 00071285     EISSN: None     Source Type: Journal    
DOI: 10.1259/bjr.72.863.10700829     Document Type: Article

References (24)

1. Withers HR, Taylor JMG, Maciejewski B. Treatment volume and tissue tolerance. Int J Radiat Oncol Biol Phys 1988;14:751-9.
2. Hendry JH. Survival of cells in mammalian tissues after low doses of irradiation: a short review. Int J Radiat Biol 1988;53:89-94.
3. Potten CS, Hendry JH, Al-Barwari SE. A cellular analysis of radiation injury in epidermis. In: CS Potten, JH Hendry, editors. Cytotoxic insult to tissue. Effects on cell lineages. Edinburgh: Churchill Livingstone, 1983:153-85.
4. Al-Barwari SE, Potten CS. Regeneration and dose-response characteristics of irradiated mouse dorsal epidermal cells. Int J Radiat Biol 1976;30:201-16.
5. Withers HR. The dose-survival relationship for irradiation of epithelial cells of mouse skin. Br J Radiol 1967;40:187-94.
6. Hendry JH. Correlation of the dose-response relationships for epidermal colony-forming units, skin reactions, and healing, in the X-irradiated mouse tail. Br J Radiol 1984;57:909-18.
7. Chen FD, Hendry JH. The radiosensitivity of microcolony and macrocolony-forming cells in mouse tail epidermis. Br J Radiol 1986;59:389-95.
8. Archambeau JO, Bennett GW, Abata JJ, Brenneis HJ. Response of swine skin to acute single exposures of X-rays: quantification of the epidermal cell changes. Radiat Res 1979;79:298-337.
9. Chen FD, Chen KY, Lee YL, Yu C. Effects of field size on the survival of pig epidermal colony-forming in vitro after electron irradiation. Int J Radiat Biol 1995;67:79-83.
10. van den Aardweg GJMJ, Hopewell JW. Manipulation of the radiosensitivity of pig epidermis by changing the concentration of oxygen and halothane in the anaesthetic gas mixture. Int J Radiat Biol 1989;56:1023-32.
11. Venselaar JLM, Brouwer WFM, Van Straaten BHM, Aalbers AHL. Intercomparison of calibration procedures for Ir-192 HDR sources in The Netherlands and Belgium. Radiother Oncol 1994; 30:155-61.
12. NCS (Netherlands Commission on Radiation Dosimetry). Recommendations for the calibration of Iridium-192 high dose rate sources. Report 7. Bilthoven, The Netherlands: City NCS reports, 1994.
13. Visser AG. An intercomparison of the accuracy of computer planning systems for brachytherapy. Radiother Oncol 1989;15:245-58.
14. Kitano Y, Okada N. Separation of the epidermal sheet by dispase. Br J Dermatol 1983;108:555-60.
15. Normand J, Karasek MA. A method for the isolation and serial propagation of keratinocytes, endothelial cells, and fibroblasts from a single punch biopsy of human skin. In Vitro Cell Dev Biol Anim 1995;31:447-55.
16. Morris GM, Hopewell JW. Cell kinetic changes in the follicular epithelium of pig skin after irradiation with single and fractionated doses of X rays. Br J Radiol 1989;62:41-7.
17. van den Aardweg GJMJ. Considerations about field-size effects after irradiation in pig epidermis as detected by a colony assay in situ. Int J Radiat Biol 1996;69:405-6.
18. Hamm PCJ, Bakker EJ, van den Berg AP, van den Aardweg GJMJ, Visser AG, Levendag PC. Single dose irradiation of Yorkshire pig skin. Comparison between brachytherapy with a single, high dose rate iridium-192 stepping source and X-rays. In preparation.
19. Morris GM, Hopewell JW. Changes in the cell kinetics of pig epidermis after single doses of X-rays. Br J Radiol 1988;61:205-11.
20. Denekamp J, Stewart FA, Douglas BG. Changes in the proliferation rate of mouse epidermis after irradiation: continuous labelling studies. Cell Tissue Kinet 1976;9:19-29.
21. Morris GM, Landuyt W, Whitehouse E, Vanuytsel L, Hopewell JW. Radiation response of mouse lip mucosal epithelium: a cell kinetic study. Int J Radiat Biol 1993;63:509-17.
22. Potten CS. The cell kinetic mechanism for radiation-induced cellular depletion of epithelial tissue based on hierarchical differences in radiosensitivity. Int J Radial Biol 1981;40:217-25.
23. Morris GM. Review article: effects of radiation on the cell proliferation kinetics of epithelial tissues -therapeutic implications. Br J Radiol 1996;69: 795-803.
24. Dörr W, Emmendorfer H, Haide E, Kummermehr J. Proliferation equivalent of "accelerated repopulation" in mouse oral mucosa. Int J Radiat Biol 1994;66:157-67.