Cost-effective one-step PCR amplification of cystic fibrosis ΔF508 fragment in a single cell for preimplantation genetic diagnosis

Prenatal Diagnosis

Volumn 19, Issue 11, 1999, Pages 1048-1051

  Tsai, Yu Hui ^a,b  

^a TAIPEI MEDICAL UNIVERSITY HOSPITAL   (Taiwan)

^b TAIPEI MEDICAL UNIVERSITY   (Taiwan)

Author keywords

CF F508; Genetic screening; PCR; Pre implantation; Single cell

Indexed keywords

BINDING PROTEIN; BUFFER; CELL DNA; DNA FRAGMENT; DOUBLE STRANDED DNA; MAGNESIUM CHLORIDE; MERCAPTOETHANOL; REDUCING AGENT; SODIUM CHLORIDE; THIOL REAGENT;

ARTICLE; CELL LYSATE; CHROMATIN; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; COOLING; COST EFFECTIVENESS ANALYSIS; CYSTIC FIBROSIS; DNA DENATURATION; FERTILIZATION IN VITRO; FLUORESCENCE; GENE AMPLIFICATION; GENE MUTATION; GENETIC DISORDER; GENETIC SCREENING; HUMAN; HUMAN CELL; INCUBATION TIME; INSTRUMENTATION; IONIC STRENGTH; LYMPHOCYTE; METHODOLOGY; POLYMERASE CHAIN REACTION; PREIMPLANTATION EMBRYO; PRIORITY JOURNAL; TEMPERATURE;

COST-BENEFIT ANALYSIS; CYSTIC FIBROSIS; DNA; DNA FRAGMENTATION; DNA PRIMERS; FEMALE; FERTILIZATION IN VITRO; GENE AMPLIFICATION; HUMANS; LYMPHOCYTES; MUTATION; POLYMERASE CHAIN REACTION; PREGNANCY; PREIMPLANTATION DIAGNOSIS;

EID: 0032710296     PISSN: 01973851     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0223(199911)19:11<1048::AID-PD703>3.0.CO;2-5     Document Type: Article

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