The relative rDNA content of a NOR determines its level of expression and its probability of becoming active. A sequential silver staining and in-situ hybridization study

Chromosome Research

Volumn 7, Issue 7, 1999, Pages 563-570

  Zurita, F ^a   Jimenez R ^a   Diaz De La Guardia R ^a   Burgos, M ^a  

^a UNIVERSITY OF GRANADA   (Spain)

Author keywords

Eliomys quercinus; Nucleolar organizer regions; Ribosomal DNA; Transcription activation

Indexed keywords

RIBOSOME DNA;

ARTICLE; CHROMOSOME NOR; CHROMOSOME PREPARATION; COMPARTMENT MODEL; IN SITU HYBRIDIZATION; NONHUMAN; PRIORITY JOURNAL; SILVER STAINING; TRANSCRIPTION REGULATION;

CHI-SQUARE DISTRIBUTION; DNA, RIBOSOMAL; IN SITU HYBRIDIZATION; NUCLEOLUS ORGANIZER REGION; SILVER STAINING;

ELIOMYS QUERCINUS;

EID: 0032707259     PISSN: 09673849     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1009297713973     Document Type: Article

References (25)

1. Amaldi F, Giacomoni D, Zito-Bignami R (1969) On the duplication of ribosomal RNA cistrons in Chinese hamster cells. Eur J Biochem 11: 419-423.
2. Burgos M, Jiménez R, Diaz de la Guardia R (1986) A rapid, simple and reliable combined method for G-banding mammalian and human chromosomes. Stain Technol 61: 257-260.
3. Cizewdki V, Sollner-Webb B (1983) A stable transcription complex directs mouse ribosomal RNA synthesis by RNA polymerase I. Nucleic Acids Res 11: 7043-7056.
4. Dauphinais C (1981) The control of ribosomal RNA transcription in lymphocytes. Evidence that the rate of chain elongation is the limiting factor. Eur J Biochem 114: 487-492.
5. Delany ME, Muscarella DE, Bloom SE (1991) Formation of nucleolar polymorphisms in trisomic chickens and subsequent microevolution of rRNA gene clusters in diploids. J Hered 82: 213-220.
6. Dembinski TC, Bell PA (1984) Glucocorticoids modify the rate of ribosomal RNA synthesis in rat thymus cells by regulating the polymerase elongation rate. J Steroid Biochem 21: 497-504.
7. Eliceiri GL (1972) The ribsomal RNA of hamster mouse hybrid cells. J Cell Biol 53: 177-184.
8. Goodpasture C, Bloom SE (1975) Visualization of nucleolar organizer regions in mammalian chromsomes using silver staining. Chromosoma 53: 37-50.
9. Honjo T, Reeder RH (1973) Preferential transcription of Xenopus Laevis rRNA in interspecies hybrids between X. laevis and X. Mulleri. J Mol Biol 80: 217-228.
10. Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer, a one step method. Experientia 36: 1014-1015.
11. Hubbel HR (1985) Silver staining as an indicator of active ribosomal genes. Stain Technol 160: 285-294.
12. Leitch IJ, Heslop-Harrison JS (1992) Physical mapping of the 18S-5, 8S-26S rRNA genes in barley by in situ hybridization. Genome 35: 1013-1018.
13. Rufas JS, Iturra P, De Souza W, Esponda P (1982) Simple silver staining procedure for the localization of nucleolus and nucleolar organizers under light and electron microscopy. Arch Biol 93: 267-276.
14. Sánchez A, Burgos M, Jiménez R, Diaz de la Guardia R (1989) Quantitative analysis of silver staining of the nucleolar organizing region in Eliomys quercinus. Genome 32: 978-982.
15. Shubert I, Künzel G (1990) Position dependent NOR activity in barley. Chromosoma 99: 352-359.
16. Sollner-Webb B, Tower J (1986) Transcription of cloned eukaryotic ribosomal RNA genes. Ann Rev Biochem 55: 801-830.
17. Suzuki H, Kurihara Y, Kanehisa T, Moriwaki K (1990) Variation in the distribution of the silver staining nucleolar organizer regions on the chromosomes of the wild mouse Mus musculus. Mol Biol Evol 7: 271-282.
18. Wachtler F, Hopman AH, Wiegant J, Schwarzacher HG (1986) On the position of nucleolus organizer regions (NORs) in interphase nuclei. Studies with a new non-autoradiographic in site hybridization method. Exp Cell Res 167: 227-240.
19. Wahl GM, Vitto L, Rubnitz J (1983) Co-amplification of rRNA genes with CAD genes in N-(phosphonacetyl)-L-aspartate-resistant Syrian hamster cells. Mol Cell Biol 3: 2066-2075.
20. Wandelt C, Grummt I (1983) Formation of stable preinitiation complexes is a prerequisite for ribosomal DNA transcription in vitro. Nucleic Acid Res 11: 3795-3809.
21. Warburton D, Henderson AS (1979) Sequential silver staining and hybridization in situ on nucleolus organizing regions in human cells. Cytogenet Cell Genet 24: 168-175.
22. Wolf S, Sameshima M, Liebhaber S, Schlessinger D (1980) Rgulation of ribosomal ribonucleic acid level in growing 3H-arrested and crisis-phase WI0S8 human diploid fibroblasts. Biochemistry 19: 3484-3490.
23. Zatsepina OV, Schofer C, Weipoltshammer K et al. (1996) The RNA polymerase-I transcription factor UBF and rDNA are located at the same major sites in both interphase and mitotic pig embryonic kidney (PK) cells. Cytogenet Cell Genet 73: 274-278.
24. Zurita F, Sánchez A, Burgos M, Jiménez R, Díaz de la Guardia R (1997) Interchromosomal intercellular and interindividual variability of NORs studied with silver staining and in situ hybridization. Heredity 78: 229-234.
25. Zurita F, Jiménez R, Burgos M, Díaz del la Guardia R (1998) Sequential silver staining and in situ hybridization reveal a direct association between rDNA levels and the expression of homologous nucleolar orgainizing regions, a hypothesis for NOR structure and function. J Cell Sci 111: 1433-1439.