Infecting larval arthropods with a chimeric, double subgenomic Sindbis virus vector to express genes of interest

BioTechniques

Volumn 27, Issue 5, 1999, Pages 908-911

  Higgs, Stephen ^a   Oray, C T ^a   Myles, K ^a   Olson, K E ^a   Beaty, B J ^a  

^a Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS VECTOR;

ARTICLE; CHIMERA; GENE EXPRESSION; GENE EXPRESSION SYSTEM; LARVA; MOSQUITO; NONHUMAN; SINDBIS VIRUS;

ARTHROPODA; SINDBIS VIRUS;

EID: 0032706862     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99275bm06     Document Type: Article

References (6)

1. Higgs, S., K.E. Olson, K.I. Kamrud, A.M. Powers and B.J. Beaty. 1997. Viral expression systems and viral infections in insects, p. 459-483. In J.M. Crampton, C.B. Beard and C. Louis (Eds.), The Molecular Biology of Disease Vectors: A Methods Manual. Chapman and Hall, London.
2. Higgs, S., D. Traul, B.S. Davis, K.I. Kamrud, C.L. Wilcox and B.J. Beaty. 1996. Green fluorescent protein expressed in living mosquitoes, without the requirement of transformation. BioTechniques 21:660-664.
3. Olson, K.E., K. Myles, R.C. Seabaugh, S. Higgs, J.O. Carlson and B.J. Beaty. Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection. Insect Mol. Biol. (In press).
4. Pinkerton, A.C., K. Michel, D.A. O'Brochta and P.W. Atkinson. Green fluorescent protein as a genetic marker in transgenic Aedes aegypti. Insect Mol. Biol. (In press).
5. Rosen, L. and D. Gubler. 1974. The use of mosquitoes to detect and propagate dengue viruses. Am. J. Trop. Med. Hyg. 23:1153-1160.
6. Seabaugh, R.C., K.E. Olson, S. Higgs, J.O. Carlson and B.J. Beaty. 1998. Development of a chimeric Sindbis virus with enhanced Per Os infection of Aedes aegypti. Virology 243:99-112.