Morphology, proliferation and apoptosis of mouse liver epithelial cells cultured as spheroids

Japanese Journal of Cancer Research

Volumn 90, Issue 10, 1999, Pages 1109-1116

  Lee, Gang Hong ^a   Osanai, Makoto ^a,b   Tokusashi, Yoshihiko ^a  

^a ASAHIKAWA MEDICAL COLLEGE   (Japan)

^b SAPPORO MEDICAL UNIVERSITY   (Japan)

Author keywords

Apoptosis; Liver epithelial cell; Morphology; Mouse; Spheroid culture

Indexed keywords

EOSIN; GLYCOGEN; HEMATOXYLIN;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL COUNT; CELL CULTURE; CELL DIVISION; CELL PROLIFERATION; CELL STRUCTURE; CORRELATION FUNCTION; ELECTRON MICROSCOPY; EPITHELIUM CELL; GENE EXPRESSION; HISTOPATHOLOGY; INTRAHEPATIC BILE DUCT; LIVER CELL; LIVER CELL CARCINOMA; MALE; MOUSE; NONHUMAN; PRECURSOR; PRIORITY JOURNAL; SPHEROID CELL; TUMOR SUPPRESSOR GENE;

EID: 0032703575     PISSN: 09105050     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1349-7006.1999.tb00685.x     Document Type: Article

References (33)

1. Evans, V. J., Earle, W. R., Wilson, E. P., Waltz, H. K. and Mackey, C. J. The growth in vitro of massive cultures of liver cells. J. Natl. Cancer Inst., 12, 1245-1266 (1952).
2. Williams, G. M., Weisburger, E. K. and Weisburger, J. H. Isolation and long-term cell culture of epithelial-like cells from rat liver. Exp. Cell Res., 69, 106-112 (1971).
3. McMahon, J. B., Richards, W. L., del Campo, A. A., Song, M. K. and Thorgeirsson, S. S. Differential effects of transforming growth factor-β on proliferation of normal and malignant rat liver epithelial cells in culture. Cancer Res., 46, 4665-4671 (1986).
4. Tsao, M. S. and Grisham, J. W. Hepatocarcinomas, cholangiocarcinomas, and hepatoblastomas produced by chemically transformed cultured rat liver epithelial cells: a light-and electron-microscopic analysis. Am. J. Pathol., 127, 168-181 (1987).
5. Farber, E. Similarities in the sequence of early histological changes induced in the liver of the rat by ethionine, 2-acetyl-aminofluorene, and 3′-methyl-4-dimethylaminoazobenzene. Cancer Res., 16, 142-148 (1956).
6. Fausto, N. Oval cells and liver carcinogenesis: an analysis of cell lineages in hepatic tumors using oncogene transfection techniques. Prog. Clin. Biol. Res., 331, 325-334 (1990).
7. Thorgeirsson, S. S. Stem cells and hepatocarcinogenesis. In "Liver Regeneration and Carcinogenesis: Molecular and Cellular Mechanisms," ed. R. L. Jirtle, pp. 99-112 (1995). Academic Press, San Diego.
8. Tsao, M. S., Smith, J. D., Nelson, K. G. and Grisham, J. W. A diploid epithelia cell line from normal adult rat liver with phenotypic properties of 'oval' cells. Exp. Cell Res., 154, 38-52 (1984).
9. Grisham, J. W., Thal, S. B. and Nagel, A. Cellular derivation of continuously cultured epithelial cells from normal liver. In "Gene Expression and Carcinogenesis in Cultured Liver," ed. L. E. Gerschenson and E. B. Thompson, pp. 1-23 (1974). Academic Press, New York.
10. Grisham, J. W. Cell types in long-term propagable cultures of rat liver. Ann. NY Acad. Sci., 349, 128-137 (1980).
11. Pitot, H. C. and Sirica, A. E. The stages of initiation and promotion in hepatocarcinogenesis. Biochim. Biophys. Acta, 605, 191-215 (1980).
12. Farber, E. and Sarma, D. S. R. Hepatocarcinogenesis: a dynamic cellular perspective. Lab. Invest., 56, 4-19 (1987).
13. Lee, G.-H., Sawada, N., Mochizuki, Y., Nomura, K. and Kitagawa, T. Immortal epithelial cells of normal C3H mouse liver in culture: possible precursor populations for spontaneous hepatocellular carcinoma. Cancer Res., 49, 403-409 (1989).
14. Lee, G.-H., Sakai, R., Nagao, M. and Kitagawa, T. Role of activated c-H-ras oncogene in the induction and progression of immortal liver epithelial cell lines derived from normal C3H mice. Int. J. Cancer, 47, 60-65 (1991).
15. Kanda, H., Tajima, H., Lee, G.-H., Nomura, K., Ohtake, K., Matsumoto, K., Nakamura, T. and Kitagawa, T. Hepatocyte growth factor transforms immortalized mouse liver epithelial cells. Oncogene, 8, 3047-3053 (1993).
16. Grasso, P. and Hardy, J. Strain difference in natural incidence and response to carcinogenesis. In "Mouse Hepatic Neoplasia," ed. W. H. Butler and P. M. Newberne, pp. 111-132 (1975). Elsevier Scientific Publ. Co., Amsterdam.
17. Landry, J., Bernier, D., Ouellet, C., Goyette, R. and Marceau, N. Spheroidal aggregate culture of rat liver cells: histotypic reorganization, biomatrix deposition, and maintenance of functional activities. J. Cell Biol., 101, 914-923 (1985).
18. Koide, N., Sakaguchi, K., Koide, Y., Asano, K., Kawaguchi, M., Matsushima, H., Takenami, T., Shinji, T., Mori, M. and Tsuji, T. Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp. Cell Res., 186, 227-235 (1990).
19. Osanai, M., Ogawa, K. and Lee, G.-H. Phenobarbital causes apoptosis in conditionally immortalized mouse hepatocytes depending on deregulated c-myc expression: characterization of an unexpected effect. Cancer Res., 57, 2896-2903 (1997).
20. Lee, G.-H., Ogawa, K. and Drinkwater, N. R. Conditional transformation of mouse liver epithelial cells: an in vitro model for analysis of genetic events in hepatocarcinogenesis. Am. J. Pathol, 147, 1811-1822 (1995).
21. Chomczynski, P. and Sacchi, N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal. Biochem., 162, 156-159 (1987).
22. Obata, M., Lee, G.-H., Kanda, H., Kitagawa, T. and Ogawa, K. Loss of heterozygosity at loci on chromosome 4, a common genetic event during the spontaneous immortalization of mouse embryonic fibroblasts. Mol. Carcinog., 19, 17-24 (1997).
23. Canman, C. E. and Kastan, M. B. Induction of apoptosis by tumor suppressor genes and oncogenes. Semin. Cancer Biol., 6, 17-25 (1995).
24. Laishes, B. A. and Farber, E. Transfer of viable putative preneoplastic hepatocytes to the livers of syngeneic host rats. J. Natl. Cancer Inst., 61, 507-512 (1978).
25. Mito, M., Ebata, H., Kusano, M., Onishi, T., Saito, T. and Sakamoto, S. Morphology and function of isolated hepatocytes transplanted into rat spleen. Transplantation, 28, 499-505 (1979).
26. Jirtle, R. L., Biles, C. and Michalopoulos, G. Morphologic and histochemical analysis of hepatocytes transplanted into syngeneic hosts. Am. J. Pathol., 101, 115-126 (1980).
27. Coleman, W. B., Wennerberg, A. E., Smith, G. J. and Grisham, J. W. Regulation of the differentiation of diploid and some aneuploid rat liver epithelial (stem-like) cells by the hepatic microenvironment. Am. J. Pathol., 142, 1373-1382 (1993).
28. Coleman, W. B., McCullough, K. D., Esch, G. L., Faris, R. A., Hixson, D. C., Smith, G. J. and Grisham, J. W. Evaluation of the differentiation potential of WB F344 rat liver epithelial stem-like cells in vivo: differentiation to hepatocytes after transplantation into dipeptidylpeptidase-IV-deficient rat liver. Am. J. Pathol., 151, 353-359 (1997).
29. Lowe, S. W., Jacks, T., Housman, D. E. and Ruley, H. E. Abrogation of oncogene-associated apoptosis allows transformation of p55-deficient cells. Proc. Natl. Acad. Sci. USA, 91, 2026-2030 (1994).
30. Wyllie, A. H. Apoptosis and carcinogenesis. Eur. J. Cell Biol., 73, 189-197 (1997).
31. Rak, J., Mitsuhashi, Y., Erdos, V., Huang, S., Filmus, J. and Kerbel, R. S. Massive programmed cell death in intestinal epithelial cells induced by three-dimensional growth conditions: suppression by mutant c-H-ras oncogene expression. J. Cell Biol., 131, 1587-1598 (1995).
32. Pitot, H. C. Progression: the terminal stage in carcinogenesis. Jpn. J. Cancer Res., 80, 599-607 (1989).
33. Braun, L., Mikumo, R. and Fausto, N. Production of hepatocellular carcinoma by oval cells: cell cycle expression of c-myc and p53 at different stages of oval cell transformation. Cancer Res., 49, 1554-1561 (1989).