Osteogenesis in vitro in rat tibia-derived osteoblasts is promoted by the homeopathic preparation, FMS*Calciumfluor

Cell Biology International

Volumn 23, Issue 1, 1999, Pages 31-40

  Palermo, Claudia ^a   Filanti, Cristina ^a   Poggi, Silvia ^a  

^a UNIVERSITY OF GENOA   (Italy)

Author keywords

Homeopathic fluoride preparation; Osteogenesis in vitro; Therapy of osteoporosis

Indexed keywords

ALKALINE PHOSPHATASE; CALCIUM 45; CELL MARKER; FLUORIDE; FMS CALCIUMFLUOR; HOMEOPATHIC AGENT; MESSENGER RNA; METALLOPROTEINASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BONE DEVELOPMENT; CELL DIFFERENTIATION; CELL MATURATION; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVATION; EXTRACELLULAR MATRIX; ISOTOPE LABELING; MINERALIZATION; NONHUMAN; OSTEOBLAST; OSTEOID; OSTEOPOROSIS; PROTEIN EXPRESSION; RAT; TIBIA; ZYMOGRAPHY;

ANIMALIA;

EID: 0032702575     PISSN: 10656995     EISSN: None     Source Type: Journal    
DOI: 10.1006/cbir.1998.0312     Document Type: Article

References (33)

1. Aubin J.E., Liu F., Malval L., Gupta A.K. Osteoblast and chondroblast differentiation. Bone. 17:1995;77S-83S.
2. Bellows C.G., Heersche J.N., Aubin J.E. The effects of fluoride on osteoblast progenitors in vitro. J Bone Min Res. 5 (Suppl 1):1990;S101-S105.
3. Bourgoin S.G., Harbour D., Poubelle P.E. Role of protein kinase C alpha, Arf and cytoplasmic calcium transients in phospholipase D activation by sodium fluoride in osteoblast-like cells. J Bone Min Res. 11:1996;1655-1665.
4. Cavezarsio, J, Imai, T, Ammann, P, Burgener, D, Bonjour, JP, 1996, Aluminum potentiates the effect of fluoride on tyrosine phosphorylation and osteoblast replication in vitro and bone mass in vivo, J Bone Min Res, 11, 46, 55.
5. Chavassieux P., Boivin G., Serre C.M., Meunier P.J. Fluoride increases rat osteoblast function and population after in vivo administration but not after in vitro exposure. Bone. 14:1993;721-725.
6. Chomczynski P., Sacchi N. Single step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Biochem. 162:1987;156-159.
7. Devogelaer J.P., Nagant D.E., Deuxchaisnes C. Fluoride therapy of type I osteoporosis. Clin Rheumatol. 00 (Suppl 3):1995;26-31.
8. 3H-thymidine incorporation in chick calvarial cell cultures and modulate the stimulatory action(s) of embryonic chick bone extract. Calcif Tissue Int. 42:1988;23-33.
9. Filanti C., Stringa E., Manduca P. Metalloproteinases 2 and 9 during rat osteogenesis. J Bone Min Res. 11 (Suppl 1):1996;M 369.
10. Gutteridge D.H., Kent G.N., Prince R.L., Nicholson G.C., Stewart G.O., Jones C.E., Bhagat C.I., Suckey B.G., Retallack R.W. Fluoride treatment of osteoporosis: cyclical non-blinded or continuous blinded studies. Osteoporos Int. 3:1993;215-217.
11. Hall B.K. Sodium fluoride as an initiator of osteogenesis from embryonic mesenchyme in vitro. Bone. 8:1987;11-116.
12. Kassem M., Mosekilde L., Eriksen E.F. Effects of fluoride on human bone cells in vitro: differences in responsiveness between stromal osteoblast precursors and mature osteoblasts. Eur J Endocrinol. 130:1994;381-386.
13. 2synthesis in a clonal osteoblast-like cell line, MOB 3-4, in vitro. J Biochem. 106:1989;8-10.
14. Kopp J.B., Robey P.G. Sodium fluoride does not increase human bone cell proliferation or protein synthesis in vitro. Calcif Tissue Int. 47:1990;221-229.
15. Liu J.H. Bisphosphonates: a review of their pharmacokinetic properties. Bone. 18:1996;75-86.
16. Manduca P., Sanguineti C., Pistone M., Boccignone E., Sanguineti F., Santolini F., Federici A. Differential expression of alkaline phosphatase in clones of human osteoblast-like cells. J Bone Min Res. 8:1993;291-300.
17. Manduca P., Palermo C., Caruso C., Brizzolara A., Sanguineti C., Filanti C., Zicca A. Rat tibial osteoblasts III: propagation in vitro is accompanied by enhancement of osteoblast phenotype. Bone. 21:1997;31-39.
18. Otha T., Wergedal J.E., Matsuyama T., Baylink D.J., Lau K.H. Phenytoin and fluoride act in concert to stimulate bone formation and to increase bone volume in adult male rats. Calcif Tissue Int. 56:1995;390-397.
19. Rapuano B.E., Bockman R.S. Protein kinases A and C positively regulate G protein-dependent activation of phosphatidylinositol-specific phospholipase C by tumor necrosis factor-alpha in MC3T3-E1 osteoblasts. J Cell Biochem. 65:1997;198-208.
20. Riggs B.L., O'Fallon W.M., Lane A., Hodgson S.F., Wahner H.W., Mush J., Chao E., Melton LJ III. Clinical trial of fluoride therapy in postmenopausal osteoporotic women: extended observations and additional analysis. J Bone Min Res. 9:1994;265-275.
21. Riggs B.L., Melton LJ III, O'Fallon W.M. Drug therapy for vertebral fractures in osteoporosis: evidence that decreases in bone turnover and increases in bone mass both determine antifracture efficacy. Bone. 18:1996;197S-201S.
22. Robey, PG, Termine, JD, 1985, Human bone cells in viro, Calcif Tissue Int, 37, 453, 460.
23. Schimmel H.W. Functional Medicine. Vols I and II, 2nd ednHeidelberg, Germany. 1996;K. F. Haug Verlag GmbH.
24. Schimmel H.W., Buthke M. Resonance homeopathy. 1994;International Association for Functional Medicine and Resonance Homeopathy Press, Baden-Baden, Germany.
25. Stein G.S., Lian J.B., Stein L., Van Wlinen A.J., Montecino M. Transcriptional controls oestoblast growth and differentiation. Physiol Rev. 76:1996;593-629.
26. Stetler-Stevenson W.G., Brown P.D., Onisto M., Levy A.T., Liotta L.A. Tissue inhibitor of metalloproteinases-2 (TIMP-2) mRNA expression in tumor cell lines and human tumor tissues. J Biol Chem. 265:1990;13,933-13,938.
27. Stringa E., Filanti C., Giunciuglio D., Albini A., Manduca P. Osteoblastic cells from rat long bone: I. Characterization of their differentiation in culture. Bone. 16:1995;663-670.
28. Susa M., Standke G.J., Jeschke M., Rohner D. Fluoroaluminate induces pertussis toxin sensitive protein phosphorylation differences in MC3T3-E1 osteoblastic and NIH3T3 fibroblastic cells. Biochem Biophys Res Comm. 235:1997;680-684.
29. Takada J., Baylink D.J., Lau K.H. Pretreatment with low doses of norethindrone potentiates the osteogenic effects of fluoride on human osteosarcoma cells. J Bone Min Res. 10:1995;1512-1522.
30. Takada J., Chevalley T., Baylink D.J., Lau K.H. Dexamethasone enhances the osteogenic effects of fluoride in human TE85 osteosarcoma cells in vitro. Calcif Tissue Int. 58:1996;355-361.
31. Talbot J.R., Fischer M.M., Farley S.M., Libanati C., Farley J., Tabuenca A., Baylink D.J. The increase in spinal bone density that occurs in response to fluoride therapy for osteoporosis is not maintained after the therapy is discontinued. Osteoporos Int. 6:1996;442-447.
32. Thomas A.B., Hashimoto H., Baylink D.J., Lau K.H. Fluoride at mitogenic concentrations increases the steady state phosphotyrosyl phosphorylation level of cellular proteins in human bone cells. J Clin Endocrinol Metab. 81:1996;2570-2578.
33. Wu L.W., Yoon H.K., Baylink D.J., Graves L.M., Lau K.H. Fluoride at mitogenic doses induces a sustained activation of p44mapk, but not p42mapk in human TE85 osteosarcoma cells. J Clin Endocrinol Metab. 82:1997;1226-1235.