Cellular artificial skin substitute produced by short period simultaneous culture of fibroblasts and keratinocytes

British Journal of Plastic Surgery

Volumn 52, Issue 7, 1999, Pages 573-578

  Kim, Byung Mook ^a,d   Suzuki, Shigehiko ^a   Nishimura, Yoshihiko ^a   Um, Soon Chan ^a   Morota, Katsuyasu ^b   Maruguchi, Tomoko ^c   Ikada, Yoshito ^a  

^a KYOTO UNIVERSITY   (Japan)

^b Gunze Research and Development Center   (Japan)

^c Maruguchi Clinic   (Japan)

^d Shimizu Hospital   (Japan)

Author keywords

Burns; Cellular artificial skin; Collagen; Fibroblasts; Keratinocytes; Mouse

Indexed keywords

BOVINE SERUM ALBUMIN; COLLAGEN;

ARTICLE; ARTIFICIAL SKIN; CELL GROWTH; CELL SUSPENSION; COCULTURE; CULTURE MEDIUM; DERMIS; EPIDERMIS; FIBROBLAST CULTURE; HUMAN; HUMAN CELL; KERATINOCYTE; PRIORITY JOURNAL;

EID: 0032701281     PISSN: 00071226     EISSN: None     Source Type: Journal    
DOI: 10.1054/bjps.1999.3162     Document Type: Article

References (35)

1. Chardack WM, Brueske DA, Santomauro AP, et al. Experimental studies on synthetic substitutes for skin and their use in the treatment of burns. Ann Surg 1962; 155: 127-32.
2. Chang WHJ, Gomez NH, Edelstein LM. Use of lyophilised pig skin for donor site cover. Br J Plast Surg 1973; 26: 147-9.
3. Elliott RA Jr, Hoehn JG. Use of commercial porcine skin for wound dressings. Plast Reconstr Surg 1973; 52: 401-5.
4. James JH, Watson ACH. The use of Opsite, a vapour permeable dressing, on skin graft donor sites. Br J Plast Surg 1975; 28: 107-10.
5. Tabis MJ, Thornton JW, Bartlett RH, et al. A new composite skin prosthesis. Burns 1980; 7: 123-30.
6. Yannas IV, Burke JF. Design of an artificial skin. I. Basic design principles. J Biomed Mater Res 1980; 14: 65-81.
7. Suzuki S, Matsuda K, Isshiki N, Tamada Y, Yoshioka K, Ikada Y. Clinical evaluation of a new bilayer 'artificial skin' composed of collagen sponge and silicone layer. Br J Plast Surg 1990; 43: 47-54.
8. Suzuki S, Matsuda K, Isshiki N, Tamada Y, Ikada Y. Experimental study of a newly developed bilayer artificial skin. Biomaterials 1990; 11: 356-60.
9. Matsuda K, Suzuki S, Isshiki N, Yoshioka K, Okada T, Ikada Y. Influence of glycosaminoglycans on the collagen sponge component of a bilayer artificial skin. Biomaterials 1990; 11: 351-5.
10. Boyce ST, Christianson DJ, Hansbrough JF. Structure of a collagen-GAG dermal skin substitute optimized for cultured human epidermal keratinocytes. J Biomed Mater Res 1988; 22: 939-57.
11. Bell E, Ehrlich HP, Buttle DJ, Nakatsuji T. Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness. Science 1981; 211: 1052-4.
12. Tinois E, Tiollier J, Gaucherand M, Dumas H, Tardy M, Thivolet J. In vitro and post-transplantation differentiation of human keratinocytes grown on the human type IV collagen film of a bilayered dermal substitute. Exp Cell Res 1991; 193: 310-19.
13. Maruguchi T, Maruguchi Y, Suzuki S, Matsuda K, Toda K, Isshiki N. A new skin equivalent: keratinocytes proliferated and differentiated on collagen sponge containing fibroblasts. Plast Reconstr Surg 1994; 93: 537-44.
14. Rheinwald JG. Serial cultivation of normal human epidermal keratinocytes. Methods Cell Biol 1980; 21A: 229-54.
15. Rheinwald JG, Green H. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 1975; 6: 331-43.
16. Rheinwald JG, Green H. Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature 1977; 265: 421-4.
17. Green H, Kehinde O, Thomas J. Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA 1979; 76: 5665-8.
18. O'Connor NE, Mulliken JB, Schlegel SB, et al. Grafting of burns with cultured epithelium prepared from autologous epidermal cells. Lancet 1981; 1: 75-8.
19. Gallico GG 3rd, O'Connor NE, Compton CC, Kehinde O, Green H. Permanent coverage of large burn wounds with autologous cultured human epithelium. N Engl J Med 1984; 311: 448-51.
20. Yannas IV, Burke JF, Gordon PL, Huang C, Rubenstein RH. Design of an artificial skin. 2. Control of chemical composition. J Biomed Mater Res 1980; 14: 107-32.
21. Burke JF, Yannas IV, Quinby WC Jr, Bondoc CC, Jung WK. Successful use of a physiologically acceptable artificial skin in the treatment of extensive burn injury. Ann Surg 1981; 194: 413-28.
22. Matsuda K, Suzuki S, Isshiki N, Ikada Y. Re-freeze dried bilayer artificial skin. Biomaterials 1993; 14: 1030-5.
23. Suzuki S, Matsuda K, Maruguchi T, Nishimura Y, Ikada Y. Further applications of 'bilayer artificial skin'. Br J Plast Surg 1995; 48: 222-9.
24. Matsuda K, Suzuki S, Isshiki N, et al. Evaluation of a bilayer artificial skin capable of sustained release of an antibiotic. Biomaterials 1992; 13: 119-22.
25. Matsuda K, Suzuki S, Isshiki N, et al. A bilayer 'artificial skin' capable of sustained release of an antibiotic. Br J Plast Surg 1991; 44: 142-6.
26. Cuono CB, Langdon R, Birchall N, Barttelbort S, McGuire J. Composite autologous-allogeneic skin replacement: development and clinical application. Plast Reconstr Surg 1987; 80: 626-37.
27. Cuono C, Langdon R, McGuire J. Use of cultured epidermal autografts and dermal allografts as skin replacement after burn injury. Lancet 1986; 1 (8490): 1123-4.
28. Boyce ST, Hansbrough JF. Biologic attachment, growth, and differentiation of cultured human epidermal keratinocytes on a graftable collagen and chondroitin-6-sulfate substrate. Surgery 1988; 103: 421-31.
29. Hansbrough JF, Boyce ST, Cooper ML, Foreman TJ. Burn wound closure with cultured autologous keratinocytes and fibroblasts attached to a collagen-glycosaminoglycan substrate. JAMA 1989; 262: 2125-30.
30. Cooper ML, Andree C, Hansbrough JF, Zapata-Sirvent RL, Spielvogel RL. Direct comparison of a cultured composite skin substitute containing human keratinocytes and fibroblasts to an epidermal sheet graft containing human keratinocytes on athymic mice. J Invest Dermatol 1993; 101: 811-19.
31. Kuroyanagi Y, Kenmochi M, Ishihara S, et al. A cultured skin substitute composed of fibroblasts and keratinocytes with a collagen matrix: preliminary results of clinical trials. Ann Plast Surg 1993; 31: 340-9.
32. Rubin JS, Osada H, Finch PW, Taylor WG, Rudikoff S, Aaronson SA. Purification and characterization of a newly identified growth factor specific for epithelial cells. Proc Natl Acad Sci USA 1989; 86: 802-6.
33. Marchese C, Rubin J, Ron D, et al. Human keratinocyte growth factor activity on proliferation and differentiation of human keratinocytes: differentiation response distinguishes KGF from EGF family. J Cell Physiol 1990; 144: 326-32.
34. Barreca A, De Luca M, Del Monte P, et al. In vitro paracrine regulation of human keratinocyte growth by fibroblast-derived insulin-like growth factors. J Cell Physiol 1992; 151: 262-8.
35. Waelti ER, Inaebnit SP, Rast HP, et al. Co-culture of human keratinocytes on post-mitotic human dermal fibroblast feeder cells: production of large amounts of interleukin 6. J Invest Dermatol 1992; 98: 805-8.