Involvement of the binuclear copper site in the proteolytic activity of polyphenol oxidase

Plant and Cell Physiology

Volumn 40, Issue 10, 1999, Pages 1029-1035

  Kuwabara, Tomohiko ^a   Katoh, Yuji ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Active oxygen; Binuclear Cu site; Dithiothreitol sensitive tetrameric protease; Fenton reaction; Polyphenol oxidase (E.C. 1.10.3.1); Spinach

Indexed keywords

ASCORBIC ACID; CATALASE; CATECHOL OXIDASE; CHELATING AGENT; DISSOLVED OXYGEN; ELECTRON TRANSFER; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME SUBSTRATE; ENZYMIC DEGRADATION; ENZYMIC HYDROLYSIS; ETHYLENE GLYCOL; HYDROGEN PEROXIDE; PHOTOSYSTEM II; POLYPHENOL DERIVATIVE; REACTION CENTER; REACTIVE OXYGEN METABOLITE; SODIUM CHLORIDE;

SPINACIA OLERACEA;

EID: 0032701274     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.pcp.a029484     Document Type: Article

References (18)

1. Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts; polyphenoloxidase in Beta vulgaris. Plant Physiol. 24: 1-15.
2. Eickman, N.C., Solomon, E.I., Larrabee, J.A., Spiro, T.G. and Lerch, K. (1978) Ultraviolet resonance Raman study of oxytyrosinase. Comparison with oxyhemocyanins. J. Amer. Chem. Soc. 100: 6529-6531.
3. Golbeck, J.H. and Cammarata, K.V. (1981) Spinach thylakoid polyphenol oxidase. Isolation, activation, and properties of the native chloroplast enzyme. Plant Physiol. 67: 977-984.
4. Kahn, V. and Andrawis, A. (1985) Inhibition of mushroom tyrosinase by tropolone. Phytochemistry 24: 905-908.
5. Kuwabara, T. (1995) The 60-kDa precursor to the dithiothreitol-sensitive protease of spinach thylakoids: structural similarities between the protease and polyphenol oxidase. FEBS Lett. 371: 195-198.
6. Kuwabara, T. and Hashimoto, Y. (1990) Purification of a dithiothreitol-sensitive tetrameric protease from spinach PSII membranes. Plant Cell Physiol. 31: 581-589.
7. Kuwabara, T., Masuda, T. and Aizawa, S. (1997) A dithiothreitol-sensitive tetrameric protease from spinach thylakoids has polyphenol oxidase activity. Plant Cell Physiol. 38: 179-187.
8. Kuwabara, T. and Suzuki, K. (1994) A prolyl endoproteinase that acts specifically on the extrinsic 18-kDa protein of photosystem II: purification and further characterization. Plant Cell Physiol. 35: 665-675.
9. Lax, A.R. and Vaughn, K.C. (1991) Colocalization of polyphenol oxidase and photosystem II proteins. Plant Physiol. 96: 26-31.
10. Lerch, K. (1981) Copper monooxygenases: tyrosinase and dopamine β-monooxygenase. In Metal Ions in Biological Systems. Edited by Sigel, H. Vol. 13. pp. 143-186. Dekker, New York.
11. Mayer, A.M. (1987) Polyphenol oxidase in plants-recent progress. Phytochemistry 26: 11-20.
12. Robinson, S.P. and Dry, I.B. (1992) Broad bean leaf polyphenol oxidase is a 60-kilodalton protein susceptible to proteolytic cleavage. Plant Physiol. 99: 317-323.
13. Shahar, T., Hennig, N., Gutfinger, T., Hareven, D. and Lifschitz, E. (1992) The tomato 66.3-kDa polyphenoloxidase gene: molecular identification and developmental expression. Plant Cell 4: 135-147.
14. Sokolenko, A., Fulgosi, H., Gal, A., Altschmied, L., Ohad, I. and Herrmann, R.G. (1995) The 64 kDa polypeptide of spinach may not be the LHCII kinase, but a lumen-located polyphenol oxidase. FEBS Lett. 371: 176-180.
15. Sommer, A., Ne'eman, E., Steffens, J.C., Mayer, A.M. and Harel, E. (1994) Import, targeting, and processing of a plant polyphenol oxidase. Plant Physiol. 105: 1301-1311.
16. Stadtman, E.R. (1993) Oxidation of free amino acids and amino acid residues in proteins by radiolysis and by metal-catalyzed reactions. Annu. Rev. Biochem. 62: 797-821.
17. Vaughn, K.C., Lax, A.R. and Duke, S.O. (1988) Polyphenol oxidase: the chloroplast oxidase with no established function. Physiol. Plant. 72: 659-665.
18. Winkler, M.E., Lerch, K. and Solomon, E.I. (1981) Competitive inhibitor binding to the binuclear copper active site in tyrosinase. J. Amer. Chem. Soc. 103: 7001-7003.