Physiological tests and bioassays: Aids or superfluities to the diagnosis of phytoplankton nutrient limitation? A comparative study in the Broads and the Meres of England

European Journal of Phycology

Volumn 34, Issue 3, 1999, Pages 253-269

  Hameed, Hameed A ^a,b   Kilinc, Sabri ^c   McGowan, Suzanne ^c   Moss, Brian ^b,c  

^a SALAHADDIN UNIVERSITY   (Iraq)

^b UNIVERSITY OF EAST ANGLIA   (United Kingdom)

^c UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

Broads; Freshwater; Meres; Nitrogen; Nutrient limitation; Phosphorus; Phytoplankton

Indexed keywords

ALKALINE PHOSPHATASE; AMMONIA; BIOASSAY; BIOMASS; EFFLUENT; ENVIRONMENT; ENZYME ACTIVITY; LAKE; NITROGEN; NUTRIENT; NUTRITION; PHOSPHORUS; PHYTOPLANKTON; TRANSPORT KINETICS; UNITED KINGDOM; WATER ANALYSIS; WATER SAMPLING;

ECOPHYSIOLOGY; GROWTH; NUTRIENT LIMITATION; PHYTOPLANKTON; WATER CHEMISTRY;

UNITED KINGDOM;

EID: 0032699747     PISSN: 09670262     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0967026299002218     Document Type: Article

References (85)

1. AXLER, R.P., GERSBERG, R.M. & GOLDMAN, C.R. (1982). Inorganic nitrogen assimilation in a subalpine lake. Limnol. Oceanogr., 27: 53-65.
2. BAKER , L.A., BREZONIK, P.L. & KRATZER, C.R. (1981). Nutrient Loading-Trophic State Relationships in Florida Lakes. Publication 56. Florida Water Resources Research Center, Gainsville.
3. BOAVIDA, M.J. & HEATH, R.T. (1984). Are the phosphatases released by Daphnia magna components of its food? Limnol. Oceanogr., 29: 641-645.
4. CARIGNAN, R. & PLANAS, D. (1994). Recognition of nutrient and light limitation in turbid mixed layers: three approaches compared in the Paraná floodplain (Argentina). Limnol. Oceanogr., 39: 580-596.
5. CHANEY, A.L. & MORBACH, E.P. (1962). Modified reagent for determination of urea and ammonia. Clin. Chem., 8: 130-132.
6. CONWAY, H.L. (1977). Interactions of inorganic nitrogen in the uptake and assimilation by marine phytoplankton. Mar. Biol., 39: 221-232.
7. DORTCH, Q. (1990). The interaction between ammonium and nitrate uptake in phytoplankton. Mar. Ecol. Prog. Ser., 61: 183-201.
8. EDMONDSON, W.T. (1979). Lake Washington and the predictability of limnological events. Arch. Hydrobiol., 13: 234-241.
9. ELSER, J.J. & KIMMEL, B.L. (1986). Alteration of phytoplankton phosphorus status during enrichment experiments: implications for interpreting nutrient enrichment bioassay results. Hydrobiologia, 133: 217-222.
10. EPPLEY, R.W., ROGERS, J.N. & MCCARTHY, J.J. (1969). Half saturation constants for uptake of nitrate and ammonium by marine phytoplankton. Limnol. Oceanogr., 14: 912-920.
11. FALKOWSKI, P.G., GREENE, R.M. & GEIDER, R.J. (1992). Physiological limitations on phytoplankton productivity in the ocean. Oceanography, 5: 83-91.
12. 2 fixation and heterocyst abundance and its relevance to the nitrogen budget of Lake 227. Can. J. Fish. Aquat. Sci., 51: 2254-2266.
13. FITZGERALD, G.P. (1968). Detection of limiting or surplus nitrogen in algae and aquatic weeds. J. Phycol., 4: 121-126.
14. FITZGERALD, G.P. (1969). Field and laboratory evaluations of bioassays for nitrogen and phosphorus with algae and aquatic weeds. Limnol. Oceanogr., 14: 206-212.
15. FITZGERALD, G.P & NELSON, T.C. (1966). Extraction and enzymatic analysis for limiting or surplus phosphorus in algae. J. Phycol., 2: 32-37.
16. FOGG, G.E. & WOLFE, M. (1954). Nitrogen metabolism of blue-green algae. Symp. Soc. Gen. Microbiol., 4: 99-125.
17. GERHART, D.Z. & LIKENS, G.E. (1975). Enrichment experiments for determining nutrient limitation: four methods compared. Limnol. Oceanogr., 20: 649-653.
18. GLIBERT, P.M. & BRONK, D.A. (1994). Release of dissolved organic nitrogen by marine diazotrophic cyanobacteria, Trichodesmium spp. Appl. Environ. Microbiol., 60: 3996-4000.
19. 2 uptake by a marine diatom. Mar. Biol., 76: 7-15.
20. GOLDMAN, J.C., DENNETT, M.R. & RILEY, C.B. (1981). Marine phytoplankton photosynthesis and transient ammonium availability. Mar. Biol. Lett., 2: 323-331.
21. GOTHAM, I.J. & REE, G.Y. (1982). Effects of nitrate and phosphate limitation on cyclostat growth of two freshwater diatoms. J. Gen. Microbiol., 128: 199-205.
22. HAMEED, H.A. (1989). Studies on the Limnology of the Trinity Broads, Norfolk. PhD Thesis, University of East Anglia, Norwich, UK.
23. HAMILTON, D.T. & TAYLOR, W.D. (1987). Short term effect of zooplankton manipulations on phosphate uptake. Can. J. Fish. Aquat. Sci., 44: 1038-1044.
24. HEALEY, F.P. (1973). Inorganic nutrient uptake and deficiency in algae. Crit. Rev. Microbiol., 3: 69-113.
25. HEALEY, F.P. (1979). Short-term responses of nutrient-deficient algae to nutrient addition. J. Phycol., 15: 289-299.
26. HEALEY, F.P. & HENDZEL, L.L. (1979). Fluorometric measurement of alkaline phosphatase activity in algae. Freshwat. Biol., 9: 429-439.
27. HEALEY, F.P. & HENDZEL, L.L. (1980). Physiological indicators of nutrient deficiency in lake phytoplankton. Can. J. Fish. Aquat. Sci., 37: 442-453.
28. HEATH, R.T. & COOK, G.D. (1975). The significance of alkaline phosphatase in a eutrophic lake. Verh. Int. Verein. Theor. Angew. Limnol., 19: 959-965.
29. HECKY, R.E. & KILHAM, P. (1988). Nutrient limitation of phytoplankton in freshwater and marine environments: a review of recent evidence on the effects of enrichment. Limnol. Oceanogr., 33: 796-822.
30. HECKY, R.E., CAMPBELL, P. & HENDZEL, L.L. (1993). The stoichiometry of carbon, nitrogen and phosphorus in particulate matter of lakes and oceans. Limnol. Oceanogr., 38: 709-724.
31. 2 fixation in Lake 227 in response to reduction of the N/P loading ratio. Can. J. Fish. Aquat. Sci., 51: 2247-2253.
32. HUBER, A.L. & KIDBY, D.K. (1984). An examination of the factors involved in determining phosphatase activities in estuarine water. 1. Analytical procedures. Hydrobiologia, 111: 3-11.
33. ISTVANOVICS, V. & HERODEK, S. (1995). Estimation of net uptake and leakage rates of orthophosphate from P-32 uptake kinetics by a linear force-flow model. Limnol. Oceanogr., 40: 17-32.
34. ISTVANOVICS, V, PETTERSON, K., RODRIGO, M.A., PIERSSON, D.C., PADISAK, J. & COLOM, W. (1993). Gloeotrichia echinulata, a colonial cyanobacterium with a unique phosphorus uptake and life strategy. J. Plankton Res., 15: 531-552.
35. JOHNES, P., MOSS, B. & PHILLIPS, G. (1996). The determination of total nitrogen and total phosphorus concentrations in freshwaters from land use, stock headage and population data: testing of a model for use in conservation and water quality management. Freshwat. Biol., 36: 451-473
36. JORDAN, C. & DINSMORE, P. (1985). Determination of biologically available phosphorus using a radiobioassay technique. Freshwat. Biol., 15 : 597-603.
37. KILHAM, S.S., KOTT, C.L. & TILMAN, D. (1977). Phosphate and silicate kinetics for the Lake Michigan diatom, Diatoma elongatum. J. Great Lakes Res., 3: 93-99.
38. KILINC, S. (1995). Limnological studies on the North West Midland Meres, with special reference to White Mere. PhD thesis, University of Liverpool, UK.
39. LEAN, D.R.S. & NALEWAJKO, C. (1979). Phosphate turnover time and phosphorus demand in large and small lakes. Arch. Hydrobiol. Beih. Ergebn. Limnol., 13: 120-132.
40. LEAN, D.R.S. & PICK, F.R. (1981). Photosynthetic response of lake plankton to nutrient enrichment: a test for nutrient limitation. Limnol. Oceanogr., 26: 1001-1019.
41. LEAN, D.R.S. & WHITE, E. (1983). Chemical and radiotracer measurement of phosphorus uptake by lake plankton. Can. J. Fish. Aquat. Sci., 40: 147-155.
42. LEAN, D.R.S., MURPHY, T.P. & PICK, F.R. (1982). Photosynthetic response of lake plankton to combined nitrogen enrichment. J. Phycol., 18: 509-521.
43. LEAN, D.R.S., ABOTT, A.P., CHALTON, M.N. & RAO, S.S. (1983). Seasonal phosphate demand for Lake Erie plankton. J. Great Lakes Res., 9: 83-91.
44. MCISAAC, J.J. & DUGDALE, R.C. (1969). The kinetics of nitrate and ammonia uptake by natural populations of marine phytoplankton. Deep Sea Res., 16: 45-57.
45. MCISAAC, J.J. & DUGDALE, R.C. (1972). Interaction of light and inorganic nitrogen in controlling nitrogen uptake in the sea. Deep Sea Res., 19: 209-232.
46. MACKERETH, F.J.H., HERON, J. & TALLING, J.F. (1978). Water Analysis and Some Revised Methods for Limnologists. Sci. Pub. Freshwat. Biol. Assoc. 33. Freshwater Biological Association, Ambleside, UK.
47. MALONE, T.C., GARSIDE, C., HAINES, K.C. & ROELS, O.A. (1975). Nitrate uptake and growth of Chaetoceros sp. in large outdoor continuous cultures. Limnol. Oceanogr., 20: 9-19.
48. MARINO, R., HOWARTH, R.W., SHAMESS, J. & PREPAS, E. (1990). Molybdenum and sulphate as controls on the abundance of nitrogen-fixing cyano-bacteria in saline lakes in Alberta. Limnol. Oceanogr., 35: 245-259.
49. MARSDEN, W.M. (1989). Lake restoration by reducing external phosphorus loading : the influence of sediment phosphorus release. Freshwat. Biol., 21: 139-162.
50. MENZEL, D.W. & RYTHER, J.H. (1961). Nutrients limiting the production of phytoplankton in the Sargasso Sea, with special reference to iron. Deep Sea Res., 7: 276-281.
51. MORRIS, D.P. & LEWIS, W.M. (1988). Phytoplankton nutrient limitation in Colorado mountain lakes. Freshwat. Biol., 20: 315-327.
52. MOSS, B. (1969). Limitation of algal growth in some Central African waters. Limnol. Oceanogr., 14: 591-601.
53. MOSS, B. (1983). The Norfolk Broadland: experiments in the restoration of a complex wetland. Biol. Rev., 58, 521-561.
54. MOSS, B., MCGOWAN, S. & CARVALHO, L. (1994). Determination of phytoplankton crops by top-down and bottom-up mechanisms in a group of English lakes, the West Midland meres. Limnol. Oceanogr., 39: 1020-1029.
55. MOSS, B., BEKLIOGLU, M., CARVALHO, L., KILINC, S., MCGOWAN, S. & STEPHEN, D. (1997). Vertically challenged limnology; contrasts between deep and shallow lakes. Hydrobiologia, 342/343: 257-267.
56. MURPHY, T.P. (1980). Ammonium and nitrate uptake in the lower Great Lakes. Can. J. Fish. Aquat. Sci., 37: 1365-1372.
57. NALEWAJKO, C. & LEAN, D.R.S. (1978). Phosphorus kinetics-algal growth relationship in batch cultures. Verh. Int. Verein Theor. Angew. Limnol., 21: 184-192.
58. PERRY, M.J. (1976). Phosphate utilization by an oceanic diatom in phosphorus-limited chemostat culture in the oligotrophic waters of the central north Pacific. Limnol. Oceanogr., 21: 88-107.
59. PETERS, R.H. (1975). Orthophosphate turnover in central European lakes. Mem. Ist. Ital. Idrobiol., 32: 297-311.
60. PICK, F.R. (1987). Interpretation of alkaline phosphatase activity in Lake Ontario. Can. J. Fish. Aquat. Sci., 44: 2087-2094.
61. PRICE, N.M., COCHLAN, W.P. & HARRISON, P.J. (1985). Time-course of the uptake of inorganic and organic nitrogen by phytoplankton in the Strait of Georgia: comparison of frontal and stratified communities. Mar. Ecol. Progr. Ser., 27: 39-53.
62. REUTER, J.E., STANFORD, L.L. & GOLDMAN, C.R. (1986). Inorganic nitrogen uptake by epilithic periphyton in a N-deficient lake. Limnol. Oceanogr., 31: 149-160.
63. REYNOLDS, C.S. (1979). The limnology of the eutrophic meres of the Shropshire-Cheshire plain: a review. Field Studies, 5: 93-173.
64. REYNOLDS, C.S. (1984). The Ecology of Freshwater Phytoplankton. Cambridge University Press, Cambridge, UK.
65. RIGLER, F.H. (1968). Further observations inconsistent with the hypothesis that the molybdenum blue method measures orthosphosphate in lake water. Limnol. Oceanogr., 13: 7-13.
66. ST AMOND, A.L., SORANNO, P.A., CARPENTER, S.R. & ELSER, J.J. (1989). Algal nutrient deficiency: growth bioassays versus physiological indicators. Lake Res. Manag., 5: 27-35.
67. SCHINDLER, D.W. (1977). Evolution of phosphorus limitation in lakes. Science, 195: 260-262.
68. SCHINDLER, D.W. (1978). Factors regulating phytoplankton production and standing crop in the world's freshwaters. Limnol. Oceanogr., 23: 478-486.
69. SCHINDLER, D.W. & FEE, E.J. (1974). Experimental lakes area; whole lakes experiment in eutrophication. J. Fish. Res. Bd. Can., 31: 937-953.
70. SETARO, F.V. & MELACK, J.M. (1984). Responses of phytoplankton to experimental nutrient enrichment in an Amazon floodplain lake. Limnol. Oceanogr., 29: 972-984.
71. SMITH, R.E.H. & KALFF, J. (1981). The effect of phosphorus limitation on algal growth rates: evidence from alkaline phosphatase. Can. J. Fish. Aquat. Sci., 38: 1421-1427.
72. STEPHEN, D., MOSS, B. & PHILLIPS, G. (1997), Do rooted macrophytes increase sediment phosphorus release? Hydrobiologia, 342/343: 27-34.
73. TAKAHASHI, M. & SAIGO, J. (1981). Nitrogen metabolism in Lake Kizaki, Japan. V. The role of nitrogen fixation in nitrogen requirement of phytoplankton. Arch. Hydrobiol., 112: 43-54.
74. TALLING, J.F. & DRIVER, D. (1961). Some problems in the estimation of chlorophyll a in phytoplankton. In Proceedings of the Conference on Primary Production Measurement, Marine & Freshwater. University of Hawaii and Atomic Energy Commission Publication TID 7633.
75. TILMAN, D. & Kilham, S.S. (1976). Phosphate and silicate uptake and growth kinetics of the diatoms Asterionella formosa and Cyclotella meneghiniana in batch and semicontinuous culture. J. Phycol., 12: 375-383.
76. TOETZ, D.W., VARGA, L.P. & LOUGHRAN, E.D. (1973). Half-saturation constants for uptake of nitrate and ammonia by reservoir plankton. Ecology, 54: 903-908.
77. TURNER, M.A., HOWELL, E.T., ROBINSON, G.G.C., HECKY, R.E. & SCHINDLER, E.U. (1994). Roles of nutrients in controlling growth of epilithon in oligotrophic lakes of low alkalinity. Can. J. Fish. Aquat. Sci., 51, 2784-2793.
78. VOLLENWEIDER, R.A. (1974). A Manual on Methods for Measuring Primary Production in Aquatic Environments, 2nd Edition. Blackwell Scientific Publications, Oxford, UK.
79. VOLLENWEIDER, R.A. (1975). Input-output models with special reference to the phosphorus loading concept in limnology. Schweiz. Z. Hydrol., 37: 53-84.
80. WATSON, R.A. & OSBORNE, P.L. (1979). An algal pigment ratio as an indicator of the nitrogen supply to phytoplankton in three Norfolk Broads. Freshwat Biol., 9: 584-594.
81. WHITE, E., LAW, K., PAYNE, G. & PICKMORE, S. (1985). Nutrient demand and availability among planktonic communities: an attempt to assess nutrient limitation to plant growth in twelve central volcanic plateau lakes. N.Z. J. Mar. Freshwat. Res., 19: 49-62.
82. WHITE, E., PAYNE, G., PICKMERE, S. & WOODS, P. (1986). Nutrient demand and availability related to growth among natural assemblages of phytoplankton. N.Z. J. Mar. Freshwat. Res., 20: 199-208.
83. WILLIAMS, P.J.B. (1973). The validity of the application of simple kinetic analysis to heterogeneous microbial populations. Limnol. Oceanogr., 18: 159-164.
84. WILLIAMS, P.J.B. (1993). Measurement of primary production from the molecular to the global scale. ICES Mar. Sci. Symp., 192: 9-19.
85. WURTSBAUGH, W.A., VINCENT, W.F., TAPIA, R.A., VINCENT, C.L. & RICHERSON, P.J. (1985). Nutrient limitation of algal growth and nitrogen fixation in a tropical alpine lake, Lake Titicaca (Peru/Bolivia). Freshwat. Biol., 15: 185-195.