Detection of hypoxia by measurement of DNA damage and repair in human lymphocytes (comet assay): A predictive variable for tumor response during chemotherapy in patients with head and neck squamous cell carcinoma

In Vivo

Volumn 13, Issue 4, 1999, Pages 343-348

  Romanet, Thierry ^a   Ré, Jean Luc ^b   De Méo, Michel ^b   Serre Debeauvlais, Françoise ^a   Lavieille, Jean Pierre ^a   Reyt, Emile ^a   Riva, Catherine ^c  

^a GRENOBLE UNIVERSITY HOSPITAL   (France)

^b Pharmacy University   (France)

^c UNIV GRENOBLE ALPES   (France)

Author keywords

5 Fluorouracil; Cisplatin; Comet assay; Drug response; Folinic acid; Hypoxia; Lymphocytes; Squamous cell carcinoma

Indexed keywords

CISPLATIN; FLUOROURACIL; FOLINIC ACID;

AEROBIC METABOLISM; ARTICLE; ASSAY; CELL HYPOXIA; CELL LEVEL; CELL POPULATION; CLINICAL ARTICLE; CONTROLLED STUDY; DNA ADDUCT; DNA DAMAGE; DNA REPAIR; DRUG CYTOTOXICITY; DRUG METABOLISM; HEAD AND NECK CANCER; HUMAN; HUMAN CELL; INTRAVENOUS DRUG ADMINISTRATION; KINETICS; LYMPHOCYTE; PREDICTION; SQUAMOUS CELL CARCINOMA; VARIANCE;

EID: 0032696978     PISSN: 0258851X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. Östling O, Johanson KJ: Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem Biophys Res Commun 123: 291-298, 1984.
2. Olive PL, Vikse CM, Durand RE: Hypoxic fractions measured in murine tumors and normal issues using the comet assay. Int J Radiat Oncol Biol Phys 29, 3: 487-491, 1994.
3. Olive PL, Johnston PJ, Banath JP, Durand RE: The comet assay: a new method to examine heterogeneity associated with solid tumors. Nature medicine 4, 1: 103-105, 1998.
4. Brox L, Gowans B, To R, Belch A: The effect of anoxia on anthracycline-induced DNA damage in the RPMI 6410 human lymphoblastoid cell line. Can J Biochem 60, 9: 873-876, 1982.
5. Zhang H, Koch CJ, Wallen CA, Wheeler KT: Radiation-induced DNA damage in tumors and normal tisues. III. Oxygen dependence of the formation of strand breaks and DNA-protein crosslinks. Radiat Res 142: 163-168, 1995.
6. Riva C, Chauvin C, Pison C, Leverve X: Cellular physiology and molecular events in hypoxia-induced apoptosis. Anticancer Res 18: 4729-4736, 1998.
7. Sotos GA, Allegra CJ: Biochemical modulation of cancer chemotherapy. In: Principles of antineoplastic drug development and pharmacology. (Schlisky RL, Milano GA, Ratin MJ, eds). New-York, Basel, Hong-Kong, Marcel Dekker, Inc 1996, pp 143-187.
8. Reed E, Parker RJ, Gill I, Bicher A, Dahholkar M, Vionnet JA, Bostick-Bruton F, Tarone R, Muggia FM: Platinum-DNA adduct in leukocytes DNA of a cohort of 49 patients with 24 different types of malignancies. Cancer Res 53: 3694-3699, 1993.
9. De Meo M, Laget M, Castegnaro M, Dumenil G: Genotoxic activity of potassium permanganate in acidic solutions. Mutation Res 260: 295-306, 1991.
10. Singh NP, Mac Coy MT, Tice RR, Schneider EL: A simple technique for quantitation of low levels of DNA damage in individual cells. Expt Cell Res 175: 184-191, 1988.
11. Bunni M, Doig MT, Donato H, Kesavan V, Priest DG: Role of methylenetetrahydrofolate depletion in methotrexate-mediated intracellular thymidilate synthesis inhibition in cultured L1210 cells. Cancer Res 48: 3398-3404, 1988.
12. Ryll T, Wagner R: Improved ion-pair high performance liquid chromatographic method for quantification of a wide variety of nucleotides and sugar-nucleotides in animal cells. J Chromatogr 570: 77-88, 1991.
13. Gelijkens CF, De Leenheer AP: Reverse-phase liquid chromatography of 5-Fluorouracil nucleosides and nucleotides with presence of quaternary ammonium ions. J Chromatogr 194: 305-314, 1992.
14. Olive PL, Frazer G Banath JP: Radiation -induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat. Res 136: 130-136, 1993.
15. Olive PL, Wlodek D, Banath JP: DNA double strand breaks measured in individual cellls subjected to gel electrophoresis.Cancer Res 51: 4671-4676, 1991.
16. Olive PL, Durand RE.: Detection of hypoxic cells in murine tumor with the use of the comet assay. J Natl Cancer Inst 84: 707-711, 1992.
17. Olive PL, Durand RE, Le Riche J, Olivotto IA, Jackson SM: Gel electrophoreis of individual cells to quantify hypoxic fraction in human breast cancers.Cancer Res 53: 733-736, 1993.
18. Demarcq C, Bunch R, Creswell D, Eastman A: The role of cell cycle progression in cisplatin-induced apoptosis in chinese hamster ovary cells. Cell Growth Diff 5: 983-993, 1994.
19. Schrober C, Gibbs JF, Yin MB, Slocum HK, Rustum YM: Cellular heterogeneity in DNA damage and growth inhibition induced by ICI D1694, Thymidylate synthase inhibitor, using single cell assays. Bioch Pharmacol 48, 5: 997-1002, 1994.
20. Cloos J, Steen I, Timmerman AJ, Van der Schans G, Snow GB, Braakhuis BJM: DNA damage processing in blood lymphocytes of head and neck squamous cell carcinoma patients is dependent on tumor site. Int J Cancer 68: 26-29, 1996.
21. Horsman MR, Khalil AA, Siemann DW, Grau C, Hill SA, Lynch M, Chaplin DJ, Overgaard J: Relationship between radiobiological hypoxia in tumours and electrode measurements of tumour oxygenation. Int J Radiat Oncol Biol Phys 29: 434-442, 1994.
22. Olive PL, Horsman MR, Grau C, Overgaard J: Detection of hypoxic cells in a C3H mouse mammary carcinoma using the comet assay. Brit J Cancer 76, 6: 694-699, 1997.
23. Rao GV, Kumar GS, Ahuja YR: Single cell gel electrophoresis on peripheral blood leukocytes of patients with oral squamous cell carcinoma. J Oral Pathol Med 26,8: 377-38, 1997.
24. Fichtinger-Schepman AJ, Van Der Velde-Visser SD, van Djik-Knijnenburg HCM, Van Oosterom AT, Baan RA and Berends F: Kinetics of the formation and removal of cispaltin- DNA adducts in blood cells and tumor tissue of cancer patients receiving chemotherapy: comparison with in vitro adduct formation. Cancer Resf 50: 7887-7894, 1990.
25. Yin MB, Rustum YM Comparative DNA strand breakage induced by Fura and FdUrd in human ileocecal adenocarcinoma (HCT-8) cells: Relevance to cell growth inhibition. Cancer Commun 3: 45-51, 1991.
26. Sedick WD, Kutler M, Brawn OE: Antifolate induced minsincorporation of deoxyuridine monophosphate into DNA: Inhibition of high molecular weight DNA synthesis in human lymphoblastoid cells. Proc Natl Acad Sci USA 78: 917-921, 1981.
27. Lonn U and Lonn S Increased levels of DNA lesions induced by leucovorin-5-Fluoropyrimidine in human colon adenocarcinoma. Cancer Res 48: 4153-4157, 1988.
28. Shirasaka T, Shimamoto Y, Ohshimo H, Saito H, Fukushima M: Metabolic basis of the synergistic antitumor activities of 5-fluorouracil and cisplatin in rodent models in vivo. Cancer Chemother. Pharmacol 32: 167-172, 1993.