Using genomic information to investigate the function of ThiI, an enzyme shared between thiamin and 4-thiouridine biosynthesis

Protein Science

Volumn 8, Issue 11, 1999, Pages 2424-2427

  Mueller, Eugene G ^a   Palenchar, Peter M ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

4 thiouridine; Adenylation; Nuv; PP(i) synthetase; RNA modification; Sulfur incorporation; Thiamin; ThiI

Indexed keywords

SULFUR; THIAMINE; URIDINE DERIVATIVE;

ADENYLATION; ARTICLE; CATALYSIS; ENZYME ANALYSIS; ENZYME STRUCTURE; ESCHERICHIA COLI; GENOME; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; RNA PROCESSING; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI;

EID: 0032696906     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.11.2424     Document Type: Article

References (15)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol 215:403-410.
2. Altschul SF, Madden TL, Schaffer AA, Zhang JH, Zhang Z, Miller W, Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucl Acids Res 25:3389-3402.
3. Bergstrom DE, Leonard NJ. 1971. Photoreaction of 4-thiouracil with cytosine. Relation to photoreactions in Escherichia coli transfer ribonucleic acids. Biochemistry 11:1-9.
4. Bork P, Koonin EV. 1994. A P-loop-like motif in a widespread ATP pyrophosphatase domain - Implications for the evolution of sequence motifs and enzyme-activity. Proteins Struct Funct Genet 20:347-355.
5. Carre DS, Thomas G, Favre A. 1974. Conformation and functioning of transfer RNAs - Crosslinked transfer RNAs as substrates for transfer RNA nucleotidyl-transferase and aminoacyl synthetases. Biochimie 56:1089-1101.
6. 1. J Mol Biol 58:367-379.
7. 1. Biochem Biophys Res Commun 37:266-271.
8. Mueller EG, Buck CJ, Palenchar PM, Barnhart LE, Paulson JL. 1998. Identification of a gene involved in the generation of 4-thiouridine in tRNA. Nucl Acids Res 26:2606.
9. Ramabhadran TV, Jagger J. 1976. Mechanism of growth delay induced in Escherichia coli by near ultraviolet-radiation. Proc Natl Acad Sci USA 73:59-63.
10. + synthetase from Bacillus subtilis. EMBO J 15:5125.
11. Ryals J, Hsu R-Y, Lipsett MN, Bremer H. 1982. Isolation of single-site Escherichia coli mutants deficient in thiamine and 4-thiouridine syntheses -Identification of a nuvC mutant. J Bacteriol 151:899.
12. Siegele DA, Hu JC. 1997. Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations. Proc Natl Acad Sci USA 94:8168-8172.
13. Taylor SV, Kelleher NL, Kinsland C, Chiu HJ, Costello CK, Backstrom AD, McLafferty FW, Begley TP. 1998. Thiamin biosynthesis in Escherichia coli - Identification of this thiocarboxylate as the immediate sulfur donor in the thiazole formation. J Biol Chem 273:16555.
14. Tesmer JG, Klem TJ, Deras ML, Davisson VJ, Smith JL. 1996. The crystal structure of GMP synthetase reveals a novel catalytic triad and is a structural paradigm for two enzyme families. Nat Struct Biol 3:74-86.
15. Webb E, Claas K, Downs DM. 1997. Characterization of thil a new gene involved in thiazole biosynthesis in Salmonella typhimurium. J Bacteriol 179:4399.