Characterisation of hydrophobic peptides by RP-HPLC from different spectral forms of LH2 isolated from Rps. palustris

Photosynthesis Research

Volumn 61, Issue 2, 1999, Pages 157-167

  Tharia, Hazel A ^a   Nightingale, Thomas D ^a   Papiz, Miroslav Z ^a   Lawless, Anna M ^a  

^a DARESBURY LABORATORY   (United Kingdom)

Author keywords

High performance liquid chromatography; Low light; Photosynthetic bacteria; Reversed phase

Indexed keywords

EID: 0032694798     PISSN: 01668595     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1006281532327     Document Type: Article

References (16)

1. Altschul SF, Gish W, Miller W, Myers EW and Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
2. Bollhagen R, Schmiedberger M and Grell E (1995) High performance liquid chromatographic purification of extremely hydrophobic peptides: Transmembrane segments. J Chromatogr A, 711: 181-186
3. Drews G (1991) Regulated development of the photosynthetic apparatus in anoxygenic bacteria. In: Bogorad I and Vasil IK (eds) The Photosynthetic Apparatus, pp 113-148. Academic Press, San Diego, CA
4. Evans MB, Hawthornthwaite AM and Cogdell RJ (1990) Isolation and characterisation of the different B800-850 lightharvesting complexes from low- and high-light grown cells of Rhodopseudomonas palustris, strain 2.1.6. BBA 1016: 71-76
5. Fowler GJS and Hunter CN (1996) The synthesis and assembly of functional high and low light LH2 antenna complexes from Rhodopseudomonas palustris in Rhodobacter sphaeroides. J Biol Chem 271: 13356-13361
6. Germeroth L, Lottspeich F, Robert B and Michel H (1993) Unexpected similarities of the B800-850 light-harvesting complex from Rhodospirillum molischianum to the B870 light-harvesting complexes from other purple photosynthetic bacteria. Biochemistry 32: 5615-5621
7. Kerfeld CA, Yeates TO and Thornber JP (1994) Purification and characterisation of the peripheral antenna of the purple-sulphur bacterium Chromatium purpuratum: Evidence of an unusual pigment-protein composition. Biochemistry 33: 2178-2184
8. Kramer H, Deinum G, Gardiner AT, Cogdell RJ, Francke C, Aartsma TJ and Amesz J (1995) Energy transfer in the photosynthetic antenna system of thepurple non-sulphur bacterium Rhodopseudomonas cryptolactis. BBA 1231: 33-40
9. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
10. Lee R, Doughty SW, Ashman K and Walker J (1996) Purification of hydrophobic integral membrane proteins from Mycoplasma hyopneumoniae by reversed-phase high performance liquid chromatography. J Chromatogr A 737: 273-279
11. McDermott G, Prince SM, Freer AA, Hawthornthwaite-Lawless AM, Papiz MZ, Cogdell RJ and Isaacs NW (1995) Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria. Nature 374: 517-521
12. Parkes-Loach PS, Sprinkle JR and Loach P (1988) Reconstitution of the B873 light-harvesting complex of Rhodospirillum rubrum from the separately isolated α- and β-polypeptides and bacteriochlorophyll a. Biochemistry 27: 2718-2727
13. Prince SM, Papiz MZ, Freer AA, McDermott G, Hawthornthwaite-Lawless AM, Cogdell RJ and Isaacs NW Apoprotein structure in the LH2 complex from Rhodopseudomonas acidophila strain 10050: Modular assembly and protein pigment interactions. J Mol Biol 268: 412-423
14. Tadros MH, Katsiou E, Hoon MA, Yurkova N and Ramji DP (1993) Cloning of a new antenna gene cluster and expression analysis of the antenna gene family of Rhodopseudomonas palustris. Eur J Biochem 217: 867-875
15. Tadros MH and Waterkamp K (1989) Multiple copies of the coding regions for the light-harvesting B800-850 α- and β-polypeptides are present in the Rhodopseudomonas palustris genome. EMBO J 8: 1303-1308
16. Zuber H and Brunisholz R (1991) Structure and function of antenna polypeptides and chlorophyll-protein complexes: Principles and variabilty. In: Scheer H (ed) The Chlorophylls, pp 627-703. CRC Press, Boca Raton, FL