Chapter 16 Correlative Light and Electron Microscopy of Mitotic Cells in Monolayer Cultures

Methods in Cell Biology

Volumn 61, Issue C, 1998, Pages 297-315

  Rieder, Conly L ^a,b,c   Cassels, Grisel ^a  

^a NEW YORK STATE DEPARTMENT OF HEALTH   (United States)

^b STATE UNIVERSITY OF NEW YORK   (United States)

^c MARINE BIOLOGICAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; CELL; CELL CULTURE; ELECTRON MICROSCOPY; HUMAN; MICROSCOPY; MICROTOMY; MITOSIS; PHYSIOLOGY; REVIEW; ULTRASTRUCTURE;

ANIMALS; CELLS; CELLS, CULTURED; HUMANS; MICROSCOPY; MICROSCOPY, ELECTRON; MICROTOMY; MITOSIS;

EID: 0032621826     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0091-679X(08)61987-1     Document Type: Article

References (59)

1. Ault, J.G., Nicklas, R.B., Tension, microtubule rearrangements, and the proper distribution of chromosomes in mitosis. Chromosoma 98 (1989), 33-39.
2. Bajer, A., Mole-Bajer, J., Formation of spindle fibers, kinetochore orientation, and behavior of the nuclear envelope during mitosis in endosperm. Chromosoma 27 (1969), 448-484.
3. Borysko, E., Roslansky, J., Methods for correlated optical and electron microscopic studies of amoebae. Ann. N. Y. Acad. Sci 78 (1959), 432-477.
4. 2) cells undergoing meiotic-like reduction division in vitro. J. Cell Biol 72 (1977), 368-379.
5. Brinkley, B.R., Cartwright, J., Ultrastructural analysis of mitotic spindle elongation in mammalian cells in vitro. J. Cell Biol 50 (1966), 416-431.
6. Brinkley, B.R., Murphy, P., Richardson, L.C., Procedure for embedding in situ selected cells cultured in vitro. J. Cell Biol 35 (1967), 279-283.
7. Bullitt, E., Rout, M.P., Kilmartin, J.V., Akey, C.W., The yeast spindle pole body is assembled around a central crystal of Spc42p. Cell 89 (1997), 1077-1086.
8. Chang, J.P., A new technique for separation of coverglass substrate from epoxy-embedded specimens for electron microscopy. J. Ultrastruct. Res 37 (1971), 370-377.
9. Eckley, D.M., Ainsztein, A.M., Mackay, A.M., Goldberg, I.G., Earnshaw, W.C., Chromosomal proteins and cytokinesis: Patterns of cleavage furrow formation and inner centromere protein positioning in mitotic heterokaryons and mid-anaphase cells. J. Cell Biol 136 (1997), 1169-1183.
10. Gorbsky, G.J., Sammak, P.J., Borisy, G.G., Chromosomes move poleward in anaphase along stationary microtubules that coordinately disassemble from their kinetochore ends. J. Cell Biol 104 (1987), 9-18.
11. Hayden, J.H., Bowser, S.S., Rieder, C.L., Kinetochores capture astral microtubules during chromosome attachment to the mitotic spindle: Direct visualization in live newt lung cells. J. Cell Biol 111 (1990), 1039-1045.
12. Hays, T.S., Salmon, E.D., Poleward force at the kinetochore in metaphase depends on the number of kinetochore microtubules. J. Cell Biol 110 (1990), 391-404.
13. Jensen, C., Bajer, A., Effects of dehydration on the microtubules of the mitotic spindle. J. Ultrastruct. Res 26 (1969), 367-386.
14. Jensen, C.G., Jensen, L.C.W., Ault, J.G., Osorio, G., Cole, R.W., Rieder, C.L., Time-lapse video light microscopic and electron microscopic observations of vertebrate epithelial cells exposed to crocidolite asbestos. Davis, J.M.G., Jurand, M.-C., (eds.) Cellular and Molecular Effects of Mineral and Synthetic Dusts and Fibers, H85, 1994, Springer-Verlag, New York, 63-78 ASI Series.
15. Khodjakov, A., Rieder, C.L., Kinetochores moving away from their associated pole do not exert a significant pushing force on the chromosome. J. Cell Biol 135 (1996), 315-327.
16. Khodjakov, A., Cole, R.W., McEwen, B.F., Buttle, K.F., Rieder, C.L., Chromosome fragments possessing only one kinetochore can congress to the spindle equator. J. Cell Biol 136 (1997), 229-240.
17. Khodjakov, A., Cole, R.W., Rieder, C.L., A synergy of technologies: Combining laser microsurgery with green fluorescent protein (GFP)-tagging. Cell Motil. Cytoskel 38 (1997), 1-8.
18. Leslie, R.J., Pickett-Heaps, J.D., Spindle microtubule dynamics following ultraviolet microbeam irradiations of mitotic diatoms. Cell 36 (1984), 717-727.
19. Li, X., Nicklas, R.B., Tension-sensitive kinetochore phosphorylation and the chromosome distribution checkpoint in praying mantid spermatocytes. J. Cell Sci 110 (1997), 537-545.
20. Marko, M., Leith, A., Sterecon: Three-dimensional reconstructions from stereoscopic contouring. J. Struct. Biol 116 (1996), 93-98.
21. McDonald, K., Mastronarde, D., O'Toole, E., Ding, R., McIntosh, J.R., Computer-based tools for morphometric analysis of mitotic spindles and other microtubule systems. EMSA Bull 21 (1991), 47-53.
22. McDonald, K.L., O'Toole, E.T., Mastronarde, D.N., McIntosh, J.R., Kinetochore microtubules in PtK cells. J. Cell Biol 118 (1992), 369-383.
23. McEwen, B.F., Heagle, A.B., Electron microscopic tomography: A tool for probing the structure and function of subcellular components. J. Imaging Syst. Technol 8 (1997), 175-187.
24. 1 kinetochore outer plate as determined by high voltage electron microscopic tomography. J. Cell Biol 120 (1993), 301-312.
25. 1 cells and its implications for the mechanisms of chromosome congression and anaphase onset. J. Cell Biol 137 (1997), 1567-1580.
26. McIntosh, J.R., Landis, S.C., The distribution of spindle microtubules during mitosis in cultured human cells. J. Cell Biol 49 (1971), 468-497.
27. 2 cells. J. Cell Biol 88 (1981), 543-553.
28. Melan, M.A., Sluder, G., Redistribution and differential extraction of soluble proteins in permeabilized cultured cells. J. Cell Sci 101 (1992), 731-743.
29. Mitchison, T.J., Polewards microtubule flux in the mitotic spindle: Evidence from photoactivation of fluorescence. J. Cell Biol 109 (1989), 637-652.
30. Mitchison, T.J., Evans, L., Schultz, E., Kirschner, M.W., Sites of microtubule assembly and disassembly in the mitotic spindle. Cell 45 (1986), 515-527.
31. Mole-Bajer, J., Bajer, A.S., Studies of selected endosperm cells with the light and electron microscope. The technique Cellule 67 (1968), 257-265.
32. Moore, M.J., Removal of glass coverslips from cultures flat embedded in epoxy resins using hydrofluoric acid. J. Microsc 104 (1975), 205-207.
33. Moritz, M., Baumfeld, B., Sedat, J.W., Alberts, B., Agard, D.A., Microtubule nucleation by gamma-tubulin containing rings in the centrosome. Nature 378 (1995), 638-640.
34. Nicklas, R.B., Ward, S.C., Elements of error correction in mitosis: Microtubule capture, release and tension. J. Cell Biol 126 (1994), 1241-1253.
35. Nicklas, R.B., Brinkley, B.R., Pepper, D.A., Kubai, D.F., Rickards, G.K., Electron microscopy of spermatocytes previously studied in life: Methods and some observations on micro-manipulated chromosomes. J. Cell Sci 35 (1979), 87-104.
36. Nicklas, R.B., Lee, G.M., Rieder, C.L., Rupp, G., Mechanically cut mitotic spindles: Clean cuts and stable microtubules. J. Cell Sci 94 (1989), 415-423.
37. Rieder, C.L., Alexander, S.P., Kinetochores are transported poleward along a single astral microtubule during chromosome attachment to the spindle in newt lung cells. J. Cell Biol 110 (1990), 81-95.
38. Rieder, C.L., Thick and thin serial sectioning for the three-dimensional reconstruction of biological ultrastructure. Methods Cell Biol 22 (1981), 215-249.
39. 1 cells:. Chromosoma 82 (1981), 693-716.
40. Rieder, C.L., Bowser, S.S., Factors which influence the light microscopic visualization of biological material in sections prepared for electron microscopy. J. Microsc 132 (1983), 71-80.
41. Rieder, C.L., Bowser, S.S., A versatile system for illuminating flat-embedded specimens during block trimming. J. Electron Microsc. Tech 1 (1984), 207-208.
42. Rieder, C.L., Cole, R.W., Perfusion chambers for high-resolution video light microscopic studies of vertebrate cell monolayers: Some considerations and a design. Methods Cell Biol 56 (1997), 253-275.
43. Rieder, C.L., Rupp, G., Bowser, S.S., Electron microscopy of semithick sections: Advantages for biomedical research. J. Electron Microsc. Tech 2 (1985), 11-28.
44. Rieder, C.L., Cole, R.W., Khodjakov, A., Sluder, G., The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores. J. Cell Biol 130 (1995), 941-948.
45. Ris, H., Witt, P.L., Structure of the mammalian kinetochore. Chromosoma 82 (1981), 153-170.
46. Robbins, E., Gonatas, N.K., The ultrastructure of a mammalian cell during the mitotic cycle. J. Cell Biol, 21, 1964, 429.
47. Shelby, R.D., Hahn, K.M., Sullivan, K.F., Dynamic elastic-behavior of alpha-satellite DNA domains visualized in situ in living human cells. J. Cell Biol 135 (1996), 545-557.
48. Skibbens, R.V., Skeen, V.P., Salmon, E.D., Directional instability of kinetochore motility during chromosome congression and segregation in mitotic newt lung cells: A push–pull mechanism. J. Cell Biol 122 (1993), 859-876.
49. Sluder, G., Rieder, C.L., Centriole number and the reproductive capacity of spindle poles. J. Cell Biol 100 (1985), 887-896.
50. Sluder, G., Miller, F.J., Rieder, C.L., The reproduction of centrosomes: Nuclear versus cytoplasmic controls. J. Cell Biol 103 (1986), 1873-1881.
51. Sluder, G., Miller, F.J., Cole, R., Rieder, C.L., Protein synthesis and the cell cycle: Centrosome reproduction in sea urchin eggs is not under translational control. J. Cell Biol 110 (1990), 2025-2032.
52. Snyder, J.A., Armstrong, L., Stonington, O.G., Spurck, T.P., Pickett-Heaps, J.D., UV-microbeam irradiations of the mitotic spindle: Spindle forces and structural analysis of lesions. Eur. J. Cell Biol 55 (1991), 122-132.
53. Soto, G.E., Young, S.J., Martone, M.E., Deerinck, T.J., Lamont, S., Carragher, B.O., Hama, K., Ellisman, M.H., Serial section electron tomography: A method for three-dimensional reconstruction of large structures. Neuroimage 1 (1994), 230-243.
54. Straight, A.F., Marshall, W.F., Sedat, J.W., Murray, A.W., Mitosis in living budding yeast: Anaphase A but no metaphase plate. Science 277 (1997), 574-578.
55. Vogel, J.M., Stearns, T., Rieder, C.L., Palazzo, R.E., Centrosomes isolated from Spisula solidissima oocytes contain rings and an unusual stoichiometric ratio of alpha and beta tubulin. J. Cell Biol 137 (1997), 193-202.
56. Wadsworth, P., Shelden, E., Rupp, G., Rieder, C.L., Biotin–tubulin incorporates into kinetochore fiber microtubules during early but not late anaphase. J. Cell Biol 109 (1989), 2257-2265.
57. Waters, J.C., Cole, R.W., Rieder, C.L., The force producing mechanism for centrosome separation during spindle formation in vertebrates is intrinsic to each aster. J. Cell Biol 122 (1993), 361-372.
58. Waters, J.C., Mitchison, T.J., Rieder, C.L., Salmon, E.D., The kinetochore microtubule minus-end disassembly associated with poleward flux produces a force that can do work. Mol. Biol. Cell 7 (1995), 1547-1558.
59. Young, S., Royer, S.M., Groves, P.M., Kinnamon, J.C., Three-dimensional reconstructions from serial micrographs using the IMB PC. J. Electron Microsc. Tech 6 (1987), 207-217.