Nanospray mass spectrometry for identification of peptides. Application of a novel interface

Acta Biochimica Polonica

Volumn 46, Issue 2, 1999, Pages 475-481

  Smoluch, Marek T ^a   Sällberg, Matti ^b   Silberring, Jerzy ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Collision induced dissociation; Electrospray ionization mass spectrometry; Nanomole electroscopy MS; Peptides; Sequencing; Tandem mass spectrometry

Indexed keywords

PEPTIDE;

ARTICLE; CHEMISTRY; MASS SPECTROMETRY; METHODOLOGY;

MASS SPECTROMETRY; PEPTIDES;

EID: 0032620892     PISSN: 0001527X     EISSN: None     Source Type: Journal    
DOI: 10.18388/abp.1999_4178     Document Type: Article

References (14)

1. Aebersold, R. (1993) Mass spectrometry of proteins and peptides in biotechnology. Curr. Opin. Biotechnol. 4, 412-419.
2. Mann, M. & Wilm, M. (1995) Electrospray mass spectrometry for protein characterization. Trends Biochem. Sci. 20, 219-224.
3. Edmonds, Ch.G. & Smith, R.D. (1990) Electrospray ionization mass spectrometry. Methods Enzymol. 193, 412-431.
4. Hofstadler, S.A., Severs, J.C., Smith, R.D., Swanek, F.D. & Ewing, A.G. (1996) Analysis of single cells with capillary electrophoresis electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Rapid Commun. Mass Spectrom. 10, 919-922.
5. Hofstadler, S.A., Swanek, F.D., Gale, D.C., Ewing, A.G. & Smith, R.D. (1995) Capillary electrophoresis-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for direct analysis of cellular proteins. Anal. Chem. 67, 1477-1480.
6. Hunt, D.F., Henderson, R.A., Shabanowitz, J., Sakaguchi, K., Michel, H., Sevilir, N., Cox, A.L., Appella, E. & Engelhard, V.H. (1992) Characterization of peptides bound to the Class I MHC molecule HLA-A2.1 by mass spectrometry. Science 255, 1261-1263.
7. Sällberg, M., Ruden, U., Magnius, L.O., Norrby, E. & Wahren, B. (1991) Rapid "tea-bag" peptide synthesis using 9-fluorenylmethoxycarbonyl (Fmoc) protected amino acids applied for antigenic mapping viral proteins. Immunol. Lett. 30, 59-68.
8. Nylander, I., Tan-No, K., Winter, A. & Silberring, J. (1995) Processing of prodynorphin-derived pep tides in striatal extracts. Identification by electrospray-ionization mass spectrometry linked to size-exclusion chromatography. Life Sci. 57, 123-129.
9. Silberring, J. (1997) Analysis of neuropeptides by size-exclusion HPLC linked to electrospray mass spectrometry; in Methods in Molecular Biology (Williams, C. & Irvine, B., eds.) pp. 129-140, Humana Press, New Jersey.
10. Roepstorff, P. & Fohlman, J. (1984) Proposal for a common nomenclature for sequence ions in mass spectra of peptides. Biomed. Mass Spectrom. 11, 601.
11. Stenfors, C., Hellman, U. & Silberring, J. (1996) Characterization of endogenous neuropeptide Y in rat hippocampus and its metabolism by nanospray mass spectrometry. J. Biol. Chem. 272, 5747-5751.
12. Wilm, M. & Mann, M. (1996) Analytical properties of the nanoelectrospray ion source. Anal. Chem. 68, 1-8.
13. Valaskovic, G.A., Kelleher, N.L., Little, D.P., Aaserud, D.J. & McLafferty, F.W. (1995) Attomole-sensitivity electrospray source for large-molecule mass spectrometry. Anal. Chem. 67, 3802-3805.
14. Wilm, M., Shevchenko, A., Houthaeve, T., Breit, S., Schweigerer, L., Fotsis, T. & Mann, M. (1996) Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry. Nature 379, 466-469.