Purification and characterization of phospholipase B from kluyveromyces lactis, and cloning of phospholipase B gene

Bioscience, Biotechnology and Biochemistry

Volumn 63, Issue 1, 1999, Pages 83-90

  Oishi, Hideki ^a   Morimoto, Takahiro ^a   Watanabe, Yasuo ^a   Tamat, Youichi ^a  

^a EHIME UNIVERSITY   (Japan)

Author keywords

Kluyveromyces lactis; Phospholipase B

Indexed keywords

ACYLTRANSFERASE; FUNGAL DNA; LYSOPHOSPHOLIPASE; PRIMER DNA;

AMINO ACID SEQUENCE; ARTICLE; COMPARATIVE STUDY; ENZYME ACTIVE SITE; ENZYMOLOGY; FUNGAL GENE; GENE EXPRESSION; GENETICS; GLYCOSYLATION; ISOLATION AND PURIFICATION; KLUYVEROMYCES; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PH; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; BASE SEQUENCE; CATALYTIC DOMAIN; CLONING, MOLECULAR; DNA PRIMERS; DNA, FUNGAL; GENE EXPRESSION; GENES, FUNGAL; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; KLUYVEROMYCES; LYSOPHOSPHOLIPASE; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0032615983     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.63.83     Document Type: Article

References (27)

1. Nishijima, M., Akamatsu, Y., and Nojima, S., Purification and properties of a membrane-bound phospholipase A1 from Mycobacterium phlei. J. Biol. Chem., 249, 5658-5667 (1974).
2. Karasawa, K., Kudo, I., Kobayashi, T., Saeki, T., Inoue, K., and Nojima, S., Purification and characterization of lysophospholipase L2 of Escherichia coli K-12. J. Biochem., 98, 1117-1125 (1985).
3. Galliard, T., Enzymatic deacylation of lipids in plant. Eur. J. Biochem., 21, 90-98 (1971).
4. 2. Biochim. Biophys. Acta, 1167, 217-231 (1993).
5. 2/lysophospholipase (phospholipase B) from guinea pig intestinal brush-border membrane. J. Biol. Chem., 264, 9470-9475 (1989).
6. 2). FEMS Lett., 410, 49-53 (1997).
7. Witt, W., Treude, R., and Werner, D., Purification of a phospholipase B inhibitor from Saccharomyces cerevisiae. Biochim. Biophys. Acta, 834, 249-255 (1985).
8. Oishi, H., Tsuda, S., Watanabe, Y., and Tamai, Y., Purification and some properties of phospholipase B from Schizosac-charomyces pombe. Biosci. Biotechnol. Biochem., 60, 1087-1092 (1996).
9. Kuwabara, Y., Maruyama, M., Watanabe, Y., Tanaka, S., and Tamai, Y., Purification and some properties of membrane-bound phospholipase B from Torulaspora delbrueckii. J. Biochem., 104, 236-241 (1988).
10. Morimoto, T., Oishi, H., Watanabe, Y., and Tamai, Y., Phospholipid deacylating activities included in yeast. Biosci. Biotechnol. Biochem., 62, 1633-1636 (1998).
11. Bligh, E. G., and Dyer, W. J., A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol., 37, 911-917 (1959).
12. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B. and Erlich, H. A., Primer-directed enzymatic amplification of DNA with thermostable DNA polymerase. Science, 239, 487-491 (1988).
13. Rothstein, R. J., One-step gene disruption in yeast. MethodsEn-zymol., 101, 202-211 (1983).
14. McMurry, W. C. and Magee, W. L., Phospholipid metabolism. Annu. Rev. Biochem., 41, 129-160 (1972).
15. Doi, O. and Nojima, S., Lysophospholipase of Escherichia coli. J. Biol. Chem., 250, 5208-5214 (1975).
16. Witt, W., Schweingruber, M. E., and Mertsching, A., Phospholipase B from the plasma membrane of Sccharomyces cerevisiae. Biochim. Biophys. Acta, 795, 108-116 (1984).
17. Lee, K. S., Patton, J. L., Fido, M., Hines, L. K., Kohlwein, S. D., Paltauf, F., Henry, S. A., and Levin D. E., The Saccharomyces cerevisiae PLB1 gene encodes a protein required for lysophospholipase and phospholipase B activity. J. Biol. Chem., 269, 19725-19730 (1994).
18. Watanabe, Y., Yashiki, Y., Sultana, G. N.-N., Maruyama, M., Kangawa, K., Tamai, Y., Cloning and sequencing of phospholipase B gene from the yeast Torulaspora delbrueckii. FEMS Microbiol. Lett., 124, 29-34 (1994).
19. Masuda, N., Kitamura, N., and Saito, K., Primary structure of protein moiety of Penicillium notatum phospholipase B deduced from the cDNA. Eur. J. Biochem., 202, 783-787 (1991).
20. 2. J. Biol. Chem., 269, 23250-23254 (1994).
21. 2. J. Biol. Chem., 271, 19225-19231 (1996).
22. Ichimasa, M., Morooka, T., and Niimura, T., Purification and properties of a membrane-bound phospholipase B from Baker’s yeast (Saccharomyces cerevisiae). J. Biochem., 95, 137-145 (1984).
23. Ichimasa, M. and Shiobara, M., Purification and some properties of soluble phospholipase B from Baker’s yeast (Saccharomyces cerevisiae). Agrie. Biol. Chem., 49, 1083-1089 (1985).
24. Maruyama, M., Kadowaki, H., Watanabe, Y., and Tamai, Y., Role of the carbohydrate moiety in phospholipase B from Torulaspora delbrueckii. Agric. Biol. Chem., 54, 599-603 (1990).
25. Yashiki, Y., Tsuda, S., Maruyama, M., Watanabe, Y., and Tamai, Y., Suppression of phospholipase B activity by its carbohydrate moiety. Biotechnol. Appl Biochem., 16, 287-295 (1992).
26. 2. Biochim. Biophys. Acta, 1167, 272-280 (1993).
27. 2. Annu. Rev. Biochem., 64, 653-688 (1995).