Mutational activation of a Gα(i) causes uncontrolled proliferation of aerial hyphae and increased sensitivity to heat and oxidative stress in Neurospora crassa

Genetics

Volumn 151, Issue 1, 1999, Pages 107-117

  Yang, Qi ^a   Borkovich, Katherine A ^a  

^a UNIVERSITY OF TEXAS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC AMP; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATASE; HYDROGEN PEROXIDE; CAROTENOID; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; FUNGUS GROWTH; GENE MUTATION; GENOTYPE; HEAT SENSITIVITY; NEUROSPORA CRASSA; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; SIGNAL TRANSDUCTION; ALLELE; DRUG EFFECT; GENETICS; HEATING; METABOLISM; MUTATION; OSMOSIS; PHYSIOLOGY;

NEUROSPORA CRASSA;

ALLELES; CAROTENOIDS; CYCLIC AMP; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEIN ALPHA SUBUNITS, GI-GO; HEATING; HYDROGEN PEROXIDE; MUTATION; NEUROSPORA CRASSA; OSMOSIS; OXIDATIVE STRESS;

EID: 0032589654     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (60)

1. ARAMAYO, R., 1996 Gene replacements at the his-3 locus of Neurospora crassa. Fungal Genet. Newsl. 43: 9-13.
2. ARMSTRONG, G. A., and J. E. HEARST, 1996 Genetics and molecular biology of carotenoid pigment biosynthesis. FASEB J. 10: 228-237.
3. BELTMAN, J., W. K. SONNENBURG and J. A. BEAVO, 1993 The role of protein phosphorylation in the regulation of cyclic nucleotide phosphodiesterases. Mol. Cell. Biochem. 127/128: 239-253.
4. BIRNBAUMER, L., 1992 Receptor-to-effector signaling through G proteins: roles for βγ dimers as well as α subunits. Cell 71: 1069-1072.
5. BORKOVICH, K. A., 1996 Signal transduction pathways and heterotrimeric G proteins, pp. 211-233 in The Mycota III: Biochemistry and Molecular Biology, edited by R. BRAMBI, and G. A. MARZLUF, Springer-Verlag, Berlin/Heidelberg.
6. BORKOVICH, K. A., F. W. FARRELLY, D. B. FINKELSTEIN, J. TAULIEN and S. LINDQUIST, 1989 HSP82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures. Mol. Cell. Biol. 9: 3919-3930.
7. BRAMLEY, P. M., and A. MACKENZIE, 1988 Regulation of carotenoid biosynthesis. Curr. Top. Cell. Regul. 29: 291-343.
8. BREWSTER, J. L., T. DE VALOIR, N. D. DWYER, E. WINTER and M. C. GUSTIN, 1993 An osmosensing signal transduction pathway in yeast. Science 259: 1760-1763.
9. BROWN, B. L., J. D. M. ALBANO, R. P. EKINS and A. M. SGHERZI, 1971 A simple and sensitive saturation assay method for the measurement of adenosine 3′:5′-cyclic monophosphate. Biochem. J. 121: 561-562.
10. BRUNO, K. S., R. ARAMAYO, P. F. MINKE, R. L. METZENBERG and M. PLAMANN. 1996 Loss of growth polarity and mislocalization of septa in a Neurospora mutant altered in the regulatory subunit of cAMP-dependent protein kinase. EMBO J. 15: 5772-5782.
11. BURNS, F., A. Z. ZHAO and J. A. BEAVO, 1996 Cyclic nucleotide phosphodiesterases: gene complexity, regulation by phosphorylation, and physiological implications. Adv. Pharmacol. 36: 29-48.
12. CASE, M. E., M. SCHWEIZER, S. R. KUSHNER and N. H. GILES, 1979 Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA. Proc. Natl. Acad. Sci. USA 76: 5259-5263.
13. CHARY, P., and D. O. NATVIG, 1989 Evidence for three differentially regulated catalase genes in Neurospora crassa: effects of oxidative stress, heat shock, and development. J. Bacteriol. 171: 2646-2652.
14. CHARY, P., D. DILLON, A. L. SCHROEDER and D. O. NATVIG, 1994 Superoxide dismutase (sod-1) null mutants of Neurospora crassa: oxidative stress sensitivity, spontaneous mutation rate and response to mutagens. Genetics 137: 723-730.
15. 1 and the mechanism of GTP hydrolysis. Science 265: 1405-1412.
16. CRUZ, A. K., H. F. TERENZI, J. A. JORGE and H. F. TERENZI, 1988 Cyclic AMP-dependent, constitutive thermotolerance in the adenylate cyclase-deficient cr-1 (crisp) mutant of Neurospora crassa. Curr. Genet. 13: 451-454.
17. DAVIS, R. H., and F. J. DESERRES, 1970 Genetic and microbiological research techniques for Neurospora crassa. Methods Enzymol. 71A: 79-143.
18. EBBOLE, D., 1990 Vectors for construction of translational fusions to β-galactosidase. Fungal Genet. Newsl. 37: 15-16.
19. FRACELIA, F., C. SCHOLLE, A. KALLIES, T. HAFKER, T. SCHRODER et al., 1997 Differential HSC70 expression during asexual development of Neurospora crassa. Microbiology 143: 3615-3624.
20. FREISSMUTH, M., and A. G. GILMAN, 1989 Mutation of Gs alpha designed to alter the reactivity of the protein with bacterial toxins, substitutions at ARG187 result in loss of GTPase activity. J. Biol. Chem. 264: 21907-21914.
21. GRAZIANO, M. P., and A. G. GILMAN, 1989 Synthesis in Escherichia coli of GTPase-deficient mutants of Gs alpha. J. Biol. Chem. 264: 15475-15482.
22. GUPTA, S. K., C. GALLEGO, J. M. LOWNDES, C. M. PLEIMAN, C. SABLE et al., 1992 Analysis of the fibroblast transformation potential of GTPase-deficient gip2 oncogenes. Mol. Cell. Biol. 12: 190-197.
23. HAFKER, T., D. TECHEL, G. STEIER and L. RENSING, 1998 Differential expression of glucose-regulated (grp78) and heat-shock-inducible (hsp70) genes during asexual development of Neurospora crassa. Microbiology 144: 37-43.
24. HANAHAN, D., 1983 Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557.
25. HANSBERG, W., and J. AGUIRRE, 1990 Hyperoxidant states cause microbial cell differentiation by cell isolation from dioxygen. J. Theor. Biol. 142: 201-221.
26. HANSBERG, W., H. DE GROOT and H. SIES, 1993 Reactive oxygen species associated with cell differentiation in Neurospora crassa. Free Rad. Biol. Med. 14: 287-293.
27. HARDING, R. W., 1973 Inhibition of conidiation and photoinduced carotenoid biosynthesis by cyclic AMP. Neurospora Newsl. 20: 20-21.
28. HERMOUET, S., T. MURAKAMI and A. M. SPIEGEL, 1993 Stable changes in expression or activation of G protein alpha i or alpha q subunits affect the expression of both beta 1 and beta 2 subunits. FEBS Lett. 327: 183-188.
29. HUDSON, T. H., J. F. ROEBER and G. L. JOHNSON, 1981 Conformational changes of adenylate cyclase regulatory proteins mediated by guanine nucleotides. J. Biol. Chem. 256: 1459-1465.
30. 1 homologue gna-1 controls multiple differentiation pathways in Neurospora crassa. Mol. Biol. Cell 7: 1283-1297.
31. i2, transduce mitogenic signals? J. Cell. Biochem. 54: 415-422.
32. KAPOOR, M., 1983 A study of the heat-shock response in Neurospora crassa. Int. J. Biochem. 15: 639-649.
33. KRINSKY, N. I., 1992 Mechanism of action of biological antioxidants. Proc. Soc. Exp. Biol. Med. 200: 248-254.
34. KRITSKY, M. S., T. A. SOKOLOVSKY, T. A. BELOZERSKAYA and E. K. CHERNYSHEVA, 1982 Relationship between cyclic AMP level and accumulation of carotenoid pigments in Neurospora crassa. Arch. Microbiol. 133: 206-208.
35. KUNKEL, T. A., J. D. ROBERTS and R. A. ZAKOUR, 1987 Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154: 367-382.
36. KURJAN, J., 1992 Pheromone response in yeast. Annu. Rev. Biochem. 61: 1097-1129.
37. LINDEN, H., M. RODRIGUEZ-FRANCO and G. MACINO, 1997 Mutants of Neurospora crassa defective in regulation of blue light perception. Mol. Gen. Genet. 254: 111-118.
38. LINDQUIST, S., and E. A. CRAIG, 1988 The heat-shock proteins. Annu. Rev. Genet. 22: 631-677.
39. MAGER, W. H., and A. J. J. DE KRUIJFF, 1995 Stress-induced transcriptional activation. Microbiol. Rev. 59: 506-531.
40. MARCHLER, G., C. SCHULLER, G. ADAM and H. RUIS, 1993 A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J. 12: 1997-2003.
41. MOORE, M. M., M. W. BREEDVELD and A. P. AUTOR, 1989 The role of carotenoids in preventing oxidative damage in the pigmented yeast, Rhodotorula mucilaginosa. Arch. Biochem. Biophys. 270: 419-431.
42. MURAYAMA, T., Y. FUJISAWA and Y. OKANO, 1995 A suppressor mutation which suppresses adenylyl cyclase mutations in Neurospora crassa. Exp. Mycol. 19: 320-323.
43. PAIETTA, J., and M. L. SARGENT, 1981 Photoreception in Neurospora crassa: correlation of reduced light sensitivity with flavin deficiency. Proc. Natl. Acad. Sci. USA 78: 5573-5577.
44. PAUL, A., S. WILSON, C. M. BELHAM, C. J. M. ROBINSON, P. H. SCOTT et al., 1997 Stress-activated protein kinases: activation, regulation and function. Cell. Signalling. 9: 403-410.
45. PLESOFSKY-VIG, N., and R. BRAMBL, 1985 Heat shock response of Neurospora crassa: protein synthesis and induced thermotolerance. J. Bacteriol. 162: 1083-1091.
46. RAU, W., and U. MITZKA-SCHNABEL, 1985 Carotenoid synthesis in Neurospora crassa. Methods Enzymol. 110: 253-267.
47. SALOMON, Y., C. LONDOS and M. RODBELL, 1974 A highly sensitive adenylate cyclase assay. Anal. Biochem. 58: 541-548.
48. SAMBROOK, J., E. F. FRITSCH and T. MANIATIS, 1989 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
49. SCHROEDER, W. A., and E. A. JOHNSON, 1995 Singlet oxygen and peroxyl radicals regulate carotenoid biosynthesis in Phaffia rhodozyma. J. Biol. Chem. 270: 18374-18379.
50. SHIMIZU, M., T. EGASHIRA and U. TAKAHAMA, 1979 Inactivation of Neurospora crassa conidia by singlet molecular oxygen generated by a photosensitized reaction. J. Bacteriol. 138: 293-296.
51. SHIN, D. Y., K. MATSUMOTO, H. IIDA, I. UNO and T. ISHIKAWA, 1987 Heat shock response of Saccharomyces cerevisiae mutants altered in cyclic AMP-dependent protein phosphorylation. Mol. Cell. Biol. 7: 244-250.
52. SIMON, M. I., M. P. STRATHMANN and N. GAUTAM, 1991 Diversity of G proteins in signal transduction. Science 252: 802-808.
53. SPRINGER, M. L., 1993 Genetic control of fungal differentiation: the three sporulation pathways in Neurospora crassa. Bioessays 15: 365-374.
54. TERENZI, H. F., M. M. FLAWIA and H. N. TORRES, 1974 A Neurospora crassa morphological mutant showing reduced adenylate cyclase activity. Biochem. Biophys. Res. Commun. 58: 990-996.
55. 1 family. J. Biol. Chem. 268: 14805-14811.
56. VANN, D. C., 1995 Electroporation-based transformation of freshly harvested conidia of Neurospora crassa. Fungal Genet. Newsl. 42A: 53.
57. VOGEL, H. J., 1964 Distribution of lysine pathways among fungi: evolutionary implications. Am. Nat. 98: 435-446.
58. WESTERGAARD, M., and H. K. MITCHELL, 1947 Neurospora V. A synthetic medium favoring sexual reproduction. Am. J. Bot. 34: 573-577.
59. YARDEN, O., M. PLAMANN, D. J. EBBOLE and C. YANOFSKY, 1992 col-1, a gene required for hyphal elongation in Neurospora crassa, encodes a protein kinase. EMBO J. 11: 2159-2166.
60. YU, J. H., J. WIESER and T. H. ADAMS, 1996 The Aspergillus FlbA RGS domain protein antagonizes G protein signaling to block proliferation and allow development. EMBO J. 15: 5184-590.