Preservation of G(i) coupling of a chimeric EP3/I-type prostaglandin (IP) receptor

Biochemical Pharmacology

Volumn 58, Issue 3, 1999, Pages 471-476

  Meyer Kirchrath, Jutta ^a   Hasse, Andreas ^a   Schrör, Karsten ^a  

^a HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

cAMP; chimeric receptor; CHO cells; EP3 receptor; G protein coupling; IP receptor

Indexed keywords

11 DEOXY 16 PHENOXY 17,18,19,20 TETRANORPROSTAGLANDIN E1; CHIMERIC PROTEIN; CYCLIC AMP; FORSKOLIN; GUANINE NUCLEOTIDE BINDING PROTEIN; INHIBITORY GUANINE NUCLEOTIDE BINDING PROTEIN; PROSTACYCLIN RECEPTOR; PROSTAGLANDIN E RECEPTOR; PROSTAGLANDIN EP3 RECEPTOR; PROSTAGLANDIN RECEPTOR; PROSTAGLANDIN RECEPTOR STIMULATING AGENT; RECEPTOR PROTEIN; RECEPTOR SUBTYPE; STIMULATORY GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG; HYBRID PROTEIN; PTGIR PROTEIN, HUMAN;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHO CELL; CONCENTRATION RESPONSE; CONTROLLED STUDY; GENETIC TRANSFECTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; SIGNAL TRANSDUCTION; AMINO ACID SEQUENCE; ANIMAL; BIOSYNTHESIS; CELL STRAIN COS1; GENETICS; HAMSTER; HUMAN; METABOLISM; MOLECULAR GENETICS;

AMINO ACID SEQUENCE; ANIMALS; CHO CELLS; COS CELLS; CRICETINAE; CYCLIC AMP; GTP-BINDING PROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; RECEPTORS, PROSTAGLANDIN; RECEPTORS, PROSTAGLANDIN E; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 0032589203     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(99)00119-7     Document Type: Article

References (22)

1. Negishi M., Sugimoto Y., Ichikawa A. Prostanoid receptors and their biological actions. Prog Lipid Res. 32:1993;417-434.
2. Negishi M., Sugimoto Y., Ichikawa A. Molecular mechanisms of diverse actions of prostanoid receptors. Biochim Biophys Acta. 1259:1993;109-120.
3. Coleman R.A., Smith W.L., Narumiya S. VIII. International union of pharmacology classification of prostanoid receptors Properties, distribution, and structure of the receptors and their subtypes . Pharmacol Rev. 46:1994;205-229.
4. 3 prostanoid receptor. FEBS Lett. 338:1994;170-174.
5. 2 receptor transduce both intracellular calcium and cAMP signals. Biochemistry. 33:1994;14496-14502.
6. 3 subtype generated by alternative messenger RNA splicing Multiple messenger systems and tissue-specific distribution . Mol Pharmacol. 48:1995;869-879.
7. 3 determines G-protein specificity. Nature. 365:1993;166-170.
8. 2. Eur J Biochem. 228:1995;23-30.
9. 3 subtype is essential for activation of GTP-binding protein. Eur J Biochem. 224:1994;161-164.
10. 3 subtype different in agonist-independent constitutive activity. J Biol Chem. 271:1996;1857-1860.
11. i coupling. FEBS Lett. 414:1997;323-326.
12. s activity. FEBS Lett. 386:1996;165-168.
13. 3 isoforms. Br J Pharmacol. 121:1997;317-323.
14. 3 receptor Cloning, expression and signal transduction . Naunyn-Schmiedeberg's Arch Pharmacol. 358:1998;160-167.
15. Katsuyama M., Sugimoto Y., Namba T., Irie A., Negishi M., Narumiya S., Ichikawa A. Cloning and expression of a cDNA for the human prostacyclin receptor. FEBS Lett. 344:1994;74-78.
16. Boie Y., Rushmore T.H., Darmon-Goodwin A., Grygorczyk R., Slipetz D.M., Metters K.M., Abramowitz M. Cloning and expression of a cDNA for the human prostanoid IP-receptor. J Biol Chem. 269:1994;12173-12178.
17. Lake R.A., Owen M.J. Transfection of the chloramphenicol-acetyltransferase gene into eucaryotic cells using diethyl-aminoethyl (DEAE)-dextran. Murray E.J. Methods in Molecular Biology. Vol 7:1991;23-34 Humana Press Inc, Clifton, NJ.
18. Aubin R., Weinfeld M., Paterson M.C. Polybrene/DMSO-assisted gene transfer. Murray E.J. Methods in Molecular Biology. Vol 7:1991;35-43 Humana Press Inc, Clifton, NJ.
19. Steiner A.L., Parker C.W., Kipnis D.M. Radioimmunoassay for cyclic nucleotides. J Biol Chem. 247:1972;1106-1113.
20. Kiriyama M., Ushikubi F., Kobayashi T., Hirata M., Sugimoto Y., Narumiya S. Ligand binding specificities of eight types and subtypes of the mouse prostanoid receptors expressed in Chinese hamster ovary cells. Br J Pharmacol. 122:1997;217-224.
21. i coupling. FEBS Lett. 414:1997;323-326.
22. 3 receptor. FEBS Lett. 401:1997;185-190.