Linker-based GnRHh-PE chimeric proteins inhibit cancer growth in nude mice

Medical Oncology

Volumn 16, Issue 1, 1999, Pages 38-45

  Ben Yehudah, Ahmi ^a   Yarkoni, Shai ^a   Nechushtan, Amotz ^a,b   Belostotsky, Ruth ^a   Lorberboum Galski, Haya ^a  

^a HEBREW UNIVERSITY   (Israel)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Cancer; Chimeric proteins; Gonadotropin releasing hormone (GnRH); Pseudomonas exotoxin A (PE); Targeting

Indexed keywords

ANTINEOPLASTIC AGENT; CHIMERIC PROTEIN; GONADORELIN; LEVO GNRH PE40; LEVO GNRH PE66; PSEUDOMONAS EXOTOXIN; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; EXOTOXIN; HYBRID PROTEIN; L GNRH PE40; L GNRH PE66 PROTEIN, CHIMERIC; L-GNRH-PE40; L-GNRH-PE66 PROTEIN, CHIMERIC; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE;

ADENOCARCINOMA; ANIMAL MODEL; ANTINEOPLASTIC ACTIVITY; ARTICLE; CANCER CELL CULTURE; CANCER CHEMOTHERAPY; CANCER GRAFT; CANCER INHIBITION; CELL KILLING; CHIMERA; DRUG EFFECT; DRUG MECHANISM; MOUSE; NONHUMAN; NUDE MOUSE; PRIORITY JOURNAL; PROTEIN STRUCTURE; PSEUDOMONAS; TARGET ORGAN; TUMOR VOLUME; ANIMAL; CANCER TRANSPLANTATION; CARCINOMA; CELL CULTURE; EVALUATION;

ADP RIBOSE TRANSFERASES; ANIMALS; ANTINEOPLASTIC AGENTS; BACTERIAL PROTEINS; CARCINOMA; EVALUATION STUDIES; EXOTOXINS; GONADOTROPIN-RELEASING HORMONE; MICE; MICE, NUDE; NEOPLASM TRANSPLANTATION; RECOMBINANT FUSION PROTEINS; TUMOR CELLS, CULTURED;

EID: 0032589064     PISSN: 07360118     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Nechushtan A, Yarkoni S, Marianovsky I, Lorberboum-Galski H. Adenocarcinoma cells are targeted by the new GnRH-PE66 chimeric toxin through specific gonadotropin-releasing hormone binding sites. J Biol Chem 1997; 272: 11597-11603.
2. King JA, Miller RP. Evolutionary aspects of gonadotropin-releasing hormone and its receptor. Cell Mol Neurol 1995; 15: 5-23.
3. Iglewski BH, Kabat D. NAD-dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin. Proc Natl Acad Sci USA 1975; 72: 2284-2288.
4. Pastan I, Chaudhary V, FitzGerald D. Recombinant toxins as novel therapeutic agents. Annu Rev Biochem 1992; 61: 331-354.
5. Allured V, Collier RJ, Carrol SF, McKay DB. Structure of exotoxin A of Pseudomonas aeruginosa at 3.0-ångström resolution. Proc Natl Acad Sci USA 1986; 83: 1320-1324.
6. Olson JC, Hamood AN, Vincent TS, Beachey EH, Iglewski BH. Identification of functional epitopes of Pseudomonas aeruginosa exotoxin A using synthetic peptides and subclone products. Mol Immunol 1990; 27: 981-993.
7. Hwang J, FitzGerald D, Adhya S, Pastan I. Functional domains of Pseudomonas exotoxin identified by deletion analysis of the gene expressed in E. coli. Cell 1987; 48: 129-136.
8. Chaudhary VK, Jinno Y Gallo MG, FitzGerald D, Pastan I. Mutagenesis of Pseudomonas exotoxin in identification of sequences responsible for the animal toxicity. J Biol Chem 1990; 265: 16306-16310.
9. Bird RE et al. Single-chain antigen-binding proteins. Science 1988; 242: 423-426.
10. Huston JS et al. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc Natl Acad Sci USA 1988; 85: 5879-5883.
11. Fishman A, Bar-Kana Y, Steinberger I, Lorberboum-Galski H. Increased cytotoxicity of interleukin 2-pseudomonas exotoxin (IL2-PE) chimeric proteins containing a targeting signal for lysosomal membranes. Biochemistry 1994; 33: 6235-6243.
12. Chung DW, Collier R. Enzymatically active peptide from the adenosine diphosphate-ribosylating toxin of Pseudomonas aeruginosa. J Mol Biol 1977; 189: 113-130.
13. Sealfon SC, Weinstein H, Millar RP. Molecular mechanisms of ligand interaction with the gonadotropin releasing receptor. Endocrine Rev 1997; 18: 180-205.
14. Grosse R, Schoneberg T, Scultz G, Gudermann T. Inhibition of gonadotropin-releasing hormone receptor signaling by expression of a splice variant of the human receptor. Mol Endo 1997; 11: 1305-1318.