Phe161 and Arg166 variants of p-hydroxybenzoate hydroxylase. Implications for NADPH recognition and structural stability

FEBS Letters

Volumn 443, Issue 3, 1999, Pages 251-255

  Eppink, Michel H M ^a   Bunthof, Christine ^a   Schreuder, Herman A ^b   Van Berkel, Willem J H ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b Hoechst Marion Roussel   (Germany)

Author keywords

Crystal structure; Flavoprotein monooxygenase; NADPH binding; p Hydroxybenzoate hydroxylase; Sequence motif; Site directed mutagenesis

Indexed keywords

4 HYDROXYBENZOATE 3 MONOOXYGENASE; ENZYME VARIANT; OXYGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UNSPECIFIC MONOOXYGENASE; ARGININE; FLAVINE ADENINE NUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PHENYLALANINE;

ARTICLE; CRYSTAL STRUCTURE; ENZYME STABILITY; ENZYME STRUCTURE; MUTATION; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS; SITE DIRECTED MUTAGENESIS; AMINO ACID SUBSTITUTION; BINDING SITE; CHEMISTRY; CRYSTALLIZATION; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; PH; PROTEIN CONFORMATION; SPECTROSCOPY; TEMPERATURE; TIME; X RAY CRYSTALLOGRAPHY;

4-HYDROXYBENZOATE-3-MONOOXYGENASE; AMINO ACID SUBSTITUTION; ARGININE; BINDING SITES; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; FLAVIN-ADENINE DINUCLEOTIDE; HYDROGEN-ION CONCENTRATION; KINETICS; NADP; PHENYLALANINE; PROTEIN CONFORMATION; PSEUDOMONAS FLUORESCENS; SPECTRUM ANALYSIS; TEMPERATURE; TIME FACTORS;

EID: 0032588643     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(98)01726-8     Document Type: Article

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