Purification of a 28kDa Babesia (Theileria) equi antigen and a 29kDa spurious erythrocyte antigen from in vitro culture through ion exchange chromatography

Veterinary Parasitology

Volumn 86, Issue 1, 1999, Pages 63-70

  Samuel, Temesgen ^a   Böse, Reinhard ^b   Schelp, Christian ^c  

^a UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^b Labor Dr Böse   (Germany)

^c Dr Bommeli AG   (Switzerland)

Author keywords

Anion exchange chromatography; Antigens; Babesia (Theileria) equi; Glycine SDS PAGE; Tricine SDS PAGE

Indexed keywords

ERYTHROCYTE ANTIGEN; PARASITE ANTIGEN;

ANIMAL CELL; ANION EXCHANGE CHROMATOGRAPHY; ANTIGEN DETECTION; ANTIGEN PURIFICATION; ARTICLE; BABESIA; BABESIOSIS; CELL CULTURE; CONTROLLED STUDY; ERYTHROCYTE; HORSE; IN VITRO STUDY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; SERODIAGNOSIS; TECHNIQUE; WESTERN BLOTTING; ANIMAL; ANIMAL DISEASE; BLOOD; IMMUNOLOGY; ION EXCHANGE CHROMATOGRAPHY; ISOELECTRIC FOCUSING; ISOLATION AND PURIFICATION; PARASITOLOGY; SENSITIVITY AND SPECIFICITY;

ANIMALS; ANTIGENS, PROTOZOAN; BABESIA; BABESIOSIS; BLOTTING, WESTERN; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERYTHROCYTES; HORSES; ISOELECTRIC FOCUSING; SENSITIVITY AND SPECIFICITY;

EID: 0032588576     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-4017(99)00115-6     Document Type: Article

References (16)

1. Ali S., Sugimoto C., Matsuda M., Sugiura T., Kanemaru T., Onuma M., Kamada M. Protein characterization of Babesia equi piroplasms isolated from infected horse erythrocytes. Parasitol. Res. 79:1993;639-643.
2. Böse R., Daemen K. Demonstration of the humoral immune responses of horses to Babesia caballi by Western blotting. Int. J. Parasitol. 22:1992;627-630.
3. Brown K.N., Grundy M.S., Hills L.A., Jarra W. Cold isohaemagglutinins in Plasmodium berghei-infected rats reacting with parasitised reticulocytes. Bull. WHO. 58:1980;449-457.
4. Brüning A. Review: Equine piroplasmosis an update on diagnosis, treatment and prevention. Br. Vet. J. 152:1996;139-151.
5. Brüning A., Phipps P., Posnett E., Canning E.U. Monoclonal antibodies against Babesia caballi and Babesia equi and their application in serodiagnosis. Vet. Parasitol. 68:1997;11-26.
6. Figueroa J.V., Buening G.M., Kinden D.A. Purification of the erythrocytic stages of Babesia bigemina from cultures. Parasitol. Res. 76:1990;675-680.
7. Goodger B.V., Wright I.G., Waltisbuhl D.J. Babesia bovis: the effect of acute inflammation and isoantibody production in the detection of babesial antigens. Experientia. 41:1985;1577-1579.
8. Holman P.J., Chieves L., Frerichs W.M., Olson D., Wagner G.G. Babesia equi erythrocytic stage continuously cultured in an enriched medium. J. Parasitol. 80:1994;232-236.
9. Knowles D.P., Perryman L.E., Goff W.L., Miller C.D., Harrington E.D., Gorham J.R. A monoclonal antibody defines a geographically conserved surface protein epitope of Babesia equi merozoites. Infect. Immun. 59:1991;2412-2417.
10. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
11. Posnett E.S., Fehrsen J., De Waal D.T., Ambrosio R.E. Detection of Babesia equi in infected horses and carrier animals using a DNA probe. Vet. Parasitol. 39:1991;19-32.
12. Richardson, B.J., Baverstock, P.R., Adams, M., 1986. Allozyme Electrophoresis, a Handbook for Animal Systematics and Population Studies. Academic Press, San Diego, pp. 22-30, pp. 249-251.
13. Samuel, T., 1998. Purification of the 28 kDa and 18 kDa antigens of Babesia equi from erythrocyte proteins by preparative isoelectric focusing and ion exchange chromatography, Doc. Med. Vet. Dissertation, School of Veterinary Medicine Hannover, Germany.
14. Schägger H., Von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100. kDa Anal. Biochem. 166:1987;368-379.
15. Schelp C., Böse R., Micha A., Hentrich B. Cloning and expression of two genes from Babesia equi merozoites and evaluation of their diagnostic potential. Appl. Parasitol. 36:1995;1-10.
16. Selander R.K., Caugant D.A., Ochman H., Musser J.M., Gilmour M.N., Whittam T.S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl. Environ. Microbiol. 51:1986;873-884.