In vivo association of pp60(v-src) and the gap-junction protein connexin 43 in v-src-transformed fibroblasts

Molecular Carcinogenesis

Volumn 25, Issue 3, 1999, Pages 187-195

  Loo, Lenora W M ^a,b   Kanemitsu, Martha Y ^c   Lau, Alan F ^b  

^a USA   (United States)

^b UNIVERSITY OF HAWAII CANCER CENTER   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cellular communication; Oncogene; Transformation; Tyrosine kinase

Indexed keywords

CONNEXIN 43; PROTEIN KINASE P60;

ANIMAL CELL; ARTICLE; CELL COMMUNICATION; CELL TRANSFORMATION; EPITOPE MAPPING; FIBROBLAST; GAP JUNCTION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; RAT; ANIMAL; CELL JUNCTION; CELL LINE; CONFOCAL MICROSCOPY; FLUORESCENT ANTIBODY TECHNIQUE; METABOLISM; PEPTIDE MAPPING; PHOSPHORYLATION; PHYSIOLOGY; PROTEIN BINDING; SERODIAGNOSIS;

ANIMALS; CELL LINE, TRANSFORMED; CONNEXIN 43; FIBROBLASTS; FLUORESCENT ANTIBODY TECHNIQUE; GAP JUNCTIONS; MICROSCOPY, CONFOCAL; ONCOGENE PROTEIN PP60(V-SRC); PEPTIDE MAPPING; PHOSPHORYLATION; PRECIPITIN TESTS; PROTEIN BINDING; RATS;

EID: 0032588111     PISSN: 08991987     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2744(199907)25:3<187::AID-MC5>3.0.CO;2-O     Document Type: Article

References (51)

1. Goodenough DA, Goliger JA, Paul DL. Connexins, connexons, and intercellular communication. Annu Rev Biochem 1996;65:475-502.
2. Kumar NM, Gilula NB. The gap junction communication channel. Cell 1996;84:381-388.
3. Yeager M, Unger VM, Falk MM. Synthesis, assembly and structure of gap junction intercellular channels. Curr Opin Struct Biol 1998;8:517-524.
4. Loewenstein WR, Rose B. The cell-cell channel in the control of growth. Seminars in Cell Biology 1992;3:59-79.
5. Yamasaki H, Naus CCG. Role of connexin genes in growth control. Carcinogenesis 1996;17:1199-1213.
6. Murray A, Fitzgerald DJ. Tumor promoters inhibit metabolic cooperation in co-cultures of epidermal and 3T3 cells. Biochem Biophys Res Commun 1979;91:395-401.
7. Yotti LP, Chang CC, Trosko JE. Elimination of metabolic cooperation in Chinese hamster cells by a tumor promoter. Science 1979;208:1089-1091.
8. Brissette JL, Kumar NM, Gilula NB, Dotto GP. The tumor promoter 12-O-tetradecanoylphorbol-13-acetate and the ras oncogene modulate expression and phosphorylation of gap junction proteins. Mol Cell Biol 1991;11:5364-5371.
9. El-Fouly MH, Trosko JE, Chang CC, Warren ST. Potential role of the human Ha-ras oncogene in the inhibition of gap junctional intercellular communication. Mol Carcinog 1989; 2:131-135.
10. Hayashi T, Nomata K, Chang CC, Ruch RJ, Trosko JE. Cooperative effects of v-myc and c-Ha-ras oncongenes on gap junctional intercellular communication and tumorigenicity in rat liver epithelial cells. Cancer Lett 1998;128: 145-154.
11. Jou YS, Layhe B, Matesic DF, et al. Inhibition of gap junctional intercellular communication and malignant transformation of rat liver epithelial cells by neu oncogene. Carcinogenesis 1995;16:311-317.
12. Atkinson MM and Sheridan JD. Reduced junctional permeability in cells transformed by different viral oncogenes. In: Bennett MVL and Spray DC, editors. Gap junctions. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press; 1985. p 205-213.
13. Azarnia R, Loewenstein WR. Intercellular communication and the control of growth. X. Alteration of junctional permeability by the src gene. A study with temperature-sensitive mutant Rous sarcoma virus. J Membr Biol 1984;82:191-205.
14. Azarnia R and Loewenstein WR. Polyomavirus middle T antigen downregulates junctional cell-to-cell communication. Mol Cell Biol 1987;7:946-950.
15. Azarnia R, Reddy S, Kmiecik TE, Shalloway D, Loewenstein WR. The cellular src gene product regulates junctional cell-cell communication. Science 1988;239:398-401.
16. Loewenstein WR. Regulation of cell-to-cell communication by phosphorylation. Biochem Soc Symp 1984;50: 43-58.
17. Saez JC, Spray DC, Nairn AC, Hertzberg EL, Greengard P, Bennett MVL. cAMP increases junctional conductance and stimulates phosphorylation of the 27-kDa principal gap junction polypeptide. Proc Natl Acad Sci USA 1986;83: 2473-2477.
18. 2+/calmodulin-dependent protein kinase II. Eur J Biochem 1990;192:263-273.
19. Kanemitsu MY, Lau AF. Epidermal growth factor stimulates the disruption of gap junctional communication and connexin43 phosphorylation independent of 12-O-tetradecanoylphorbol 13-acetate-sensitive protein Kinase C: The possible involvement of mitogen-activated protein kinase. Mol Biol Cell 1993;4:837-848.
20. Lau AF, Kanemitsu MY, Kurata WE, Danesh S, Boynton AL. Epidermal growth factor disrupts gap-junctional communication and induces phosphorylation and connexin43 on serine. Mol Biol Cell 1992;3:865-874.
21. Warn-Cramer BJ, Trevor Cottrell G, Burt JM, Lau A. Regulation of connexin43 gap junction intercellular communication by mitogen-activated protein kinase. J Biol Chem 1998;273:9188-9196.
22. Crow DS, Beyer EC, Paul DL, Kobe SS, Lau AF. Phosphorylation of connexin43 gap junction protein in uninfected and Rous sarcoma virus-transformed mammalian fibroblasts. Mol Cell Biol 1990;10:1754-1763.
23. Filson AJ, Arzarnia R, Beyer EC, Loewenstein WR, Brugge JS. Tyrosine phosphorylation of a gap junction protein correlates with inhibition of cell-to-cell communication. Cell Growth Diff 1990;1:661-668.
24. v-src. Oncogene 1994;9:329-335.
25. Parsons JT, Weber MJ. Genetics of src: Structure and functional organization of a protein tyrosine kinase. Curr Top Microbiol lmmunol 1989;147:79-127.
26. Brown MT, Cooper JA. Regulation, substrates and functions of src. Biochimica et Biophysica Acta 1996;1287: 121-149.
27. Thomas SM and Brugge JS. Cellular functions regulated by Src family kinases. Ann Rev Cell Dev Biol 1997;13: 513-609.
28. Kamps MP, Buss JE, Sefton BM. Rous sarcoma virus transforming protein lacking myristic acid phosphorylates known polypeptide substrates without inducing transformation. Cell 1986;45:105-112.
29. Loewenstein WR, Azarnia R. Regulation of intercellular communication and growth by the cellular src gene. Ann NY Acad Sci 1988;551:337-346.
30. src prevents both myristolation and morphological transformation. Proc Natl Acad Sci USA 1985; 82:4625-4628.
31. Beyer EC, Paul DL, Goodenough DA. Connexin43: A protein from rat heart homologous to a gap junction protein from liver. J Cell Biol 1987;105:2621-2629.
32. Kanemitsu MY, Loo LWM, Simon S, Lau AF, Eckhart W. Tyrosine phosphorylation of connexin43 by v-Src is mediated by SH2 and SH3 domain interactions. J Biol Chem 1997;272:22824-22831.
33. src-mediated phosphorylation of connexin43, a gap junction protein. J Biol Chem 1995;270:12751-12761.
34. Laemmli UK Cleavage of structural proteins during the assembly of the head bacteriophage T4. Nature 1970;227: 680-685.
35. Broome MA, Hunter T. The PDGF receptor phosphorylates Tyr 138 in the c-Src SH3 domain in vivo reducing peptide ligand binding. Oncogene 1997;14:17-34.
36. Kamps MP, Sefton BM. Acid and base hydrolysis of phosphoproteins bound to immobilon facilitates analysis of phosphoamino acids in gel-fractionated proteins. Anal Biochem 1989;176:22-27.
37. Boyle WJ, van der Geer P, Hunter T. Phosphopeptide mapping and phosphoamino acid analysis by two dimensional separation on thin-layer cellulose plates. Methods Enzymol 1991;201:110-149.
38. Hertzberg EL, Disher RM, Tiller AA, Zhou Y, Cook RG. Topology of the M, 27,000 liver gap junction protein. J Biol Chem 1988;263:19105-19111.
39. Willingham MC, Jay G, Pastan I. Localization of the ASV src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistry. Cell 1979; 18:125-134.
40. v-src-induced inhibition of communication. Cell Regul 1990;1:989-1002.
41. src and selected cytoskeletal proteins within adhesion plaques and junctions of Rous sarcoma virus-transformed rat cells. J Cell Biol 1981;89:525-535.
42. Crow DS, Kurata WE, Lau AF. Phosphorylation of connexin43 in cells containing mutant srconcogene. Oncogene 1992;7:999-1003.
43. src neutralize the tyrosine specific kinase activity. J Biol Chem 1983;258: 11219-11228.
44. Superti-Furga G, Courtneidge SA. Structure-function relationships in Src family and related protein tyrosine kinases. Bioessays 1995;17:321-330.
45. src direct stable association with tyrosine phosphorylated proteins p130 and p110. EMBO J 1991;10:1689-1698.
46. FAK. Mol Cell Biol 1994;14:147-155.
47. Rozakis-Adcock M, McClade J, Mbamalu G, et al. Association of the Shc and Grb2/Sem5 SH2-containing proteins is implicated in activation of the Ras pathway by tyrosine kinases. Nature 1992;360:689-692.
48. Esinduy CB, Chang CC, Trosko JE, et al. In vitro growth inhibition of neoplastically transformed cells by non-transformed cells: Requirement for gap junctional intercellular communication. Carcinogenesis 1995;16: 915-921.
49. Cesencummings K, Fernstrom MJ, Malkinson AM, et al. Frequent reduction of gap junctional intercellular communication and connexin43 expression in human and mouse lung carcinoma cells. Carcinogenesis 1998; 19:61-67.
50. Martyn KD, Kurata WE, Warn-Cramer BJ, Burt JM, TenBroek E, Lau AF. Immortalized connexin43 knockout cell lines display a subset of biological properties associated with the transformed phenotype. Cell Growth Differ 1997;8: 1015-1027.
51. Hirschi KK, Xu CE, Tsukamoto T, Sager R. Gap junction genes Cx26 and Cx43 individually suppress the cancer phenotype of human mammary carcinoma cells and restore differentiation potential. Cell Growth Differ 1996;7: 861-870.