Flt3L induces the ex-vivo amplification of umbilical cord blood committed progenitors and early stem cells in short-term cultures

British Journal of Haematology

Volumn 106, Issue 1, 1999, Pages 133-141

  De Felice, Lidia ^a   Di Pucchio, Tiziana ^a   Mascolo, Maria Grazia ^a   Agostini, Francesca ^a   Breccia, Massimo ^a   Guglielmi, Cesare ^a   Ricciardi, Maria Rosaria ^a   Tafuri, Agostino ^a   Screnci, Maria ^a   Mandelli, Franco ^a   Arcese, William ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Ex vivo amplification; Flt3L; Haemopoietic stem cells; Quiescent cells; Umbilical cord blood

Indexed keywords

ARTICLE; CELL CULTURE; CELL PROLIFERATION; HEMATOPOIETIC STEM CELL; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; UMBILICAL CORD BLOOD; CYTOLOGY; FETUS BLOOD; FLOW CYTOMETRY; IMMUNOPHENOTYPING; NEWBORN;

CD135 ANTIGEN; CD34 ANTIGEN; FLT3 PROTEIN, HUMAN; ONCOPROTEIN; PROTEIN TYROSINE KINASE;

ANTIGENS, CD34; CELLS, CULTURED; FETAL BLOOD; FLOW CYTOMETRY; FMS-LIKE TYROSINE KINASE 3; HEMATOPOIETIC STEM CELLS; HUMANS; IMMUNOPHENOTYPING; INFANT, NEWBORN; PROTO-ONCOGENE PROTEINS; RECEPTOR PROTEIN-TYROSINE KINASES;

EID: 0032588058     PISSN: 00071048     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2141.1999.01519.x     Document Type: Article

References (34)

1. Andreeff, M., Darzynkiewicz, Z., Sharpless, T.K., Clarkson, B.D. & Melamed, M.R. (1980) Discrimination of human leukemia subtypes by flow cytometric analysis of cellular DNA and RNA. Blood, 55, 282-293.
2. Baum, C.M., Weissman, I.L., Tsukamoto, A.S., Buckle, A.M. & Peault, B. (1992) Isolation of a candidate human hematopoietic stem-cell population. Proceedings of the National Academy of Sciences of the United States of America, 89, 2804-2808.
3. Bhatia, M., Bonnet, D., Kapp, U., Wang, J.C.Y., Murdoch, B. & Dick, J.E. (1997) Quantitative analysis reveals expansion of human hematopoietic repopulating cells after short-term ex vivo culture. Journal of Experimental Medicine, 186, 619-624.
4. Broxmeyer, H.E., Lu, L., Cooper, S., Ruggieri, L., Li, Z.H. & Lyman, S.D. (1995) Flt3 ligand stimulates/costimulates the growth of myeloid stem/progenitor cells. Experimental Hematology, 23, 1121-1129.
5. Brugger, W., Heimfeld, S., Berenson, R.J., Mertelsman, R. & Kanz, L. (1995) Reconstitution of hematopoiesis after high-dose chemotherapy by autologous progenitor cells generated ex vivo. New England Journal of Medicine, 333, 283-287.
6. Craig, W., Kay, R., Cutler, R.L. & Lansdorp, P.M. (1993) Expression of Thy-1 on human hematopoietic progenitor cells. Journal of Experimental Medicine, 177, 1331-1342.
7. + subpopulations in umbilical cord blood and identification of long-term culture-initiating cells present in two subset. Stem Cells, 14, 566-576.
8. Gluckman, E., Rocha, V., Boyer Chammard, A., Locatelli, F., Arcese, W., Pasquini, R., Ortega, J., Souillet, G., Ferreira, E., Laporte, J.P., Fernandez, M. & Chastang, C. (1997) Outcome of cord-blood transplantation from related and unrelated donors. Eurocord Transplant Group and the European Blood and Marrow Transplantation Group. New England Journal of Medicine, 337, 373-381.
9. + cells isolated in subcompartiments of the G0/G1 phase of the cell cycle. Blood, 90, 4384-4393.
10. Jones, R.J., Wagner, J.E., Celano, P., Zicha, M.S & Sharkis, S.J. (1990) Separation of pluripotent haematopoietic stem cells from spleen colony-forming cells. Nature, 347, 188-189.
11. Knobel, K.M., McNally, M.A., Berson, A.E., Rood, D., Chen, K., Kilinski, L., Tran, K., Okarma, T.B. & Lebkowski, J.S. (1994) Long-term reconstitution of mice after ex vivo expansion of bone marrow cells: differential activity of cultured bone marrow and enriched stem cell populations. Experimental Hematology, 22, 1277-1235.
12. Kurtzberg, J., Laughlin, M., Graham, M.L., Smith, C., Olson, J.F., Halperin, E.C., Ciocci, G., Carrier, C., Stevens, C.E. & Rubinstein, P. (1996) Placental blood as a source of hematopoietic stem cells for transplantation into unrelated recipients. New England Journal of Medicine, 335, 157-166.
13. + hematopoietic progenitor cells residing in G0. Blood, 90, 658-668.
14. Lyman, S.D., James, L., Johnson, L., Brasel, K., de Vries, P., Escobar, S.S., Downey, H., Splett, R.R., Beckman, M.P. & McKenna, H.J. (1994) Cloning of the human homologue of the murine flt3 ligand: a growth factor for early hematopoietic progenitor cells. Blood, 83, 2795-2801.
15. + hematopoietic progenitor cells. Blood, 86, 3413-3420.
16. Ohmizono, Y., Sakabe, H., Kimura, T., Tanimukani, S., Matsumura, T., Miyazaki, H., Lyman, S.D. & Sonoda, Y. (1997) Thrombopoietin augments ex vivo expansion of human cord blood-derived hematopoietic progenitors in combination with stem cell factor and flt3 ligand. Leukemia, 11, 524-530.
17. Peters, S.O., Kittel, E.L.W., Ramshaw, H.S. & Quesenberry, P.Y. (1995) Murine marrow cells expanded in culture with IL-3, IL-6, IL-11 and SCF acquire an engraftment defect in normal hosts. Experimental Hematology, 23, 461-469.
18. Petzer, A.L., Hoge, D.E., Lansdorp, P.M., Reid, D.S. & Eaves, C.J. (1996) Self-renewal of primitive human hematopoietic cells (long-term-culture-initiating cells) in vitro and their expansion in defined medium. Proceedings of the National Academy of Sciences of the United States of America, 96, 1470-1474.
19. Piacibello, W., Sanavio, P., Garetto, L., Severino, A., Bergandi, D., Ferrario, J., Fagioli, F., Berger, M. & Aglietta, M. (1997) Extensive amplification and self-renewal of primitive hematopoietic stem cells from cord blood. Blood, 89, 2644-2653.
20. Rubinstein, P., Carrier, C., Scaradavon, A., Kurtzberg, J., Adamson, J., Migliaccio, A.R., Berkowitz, R.L., Cabbad, M., Dobrilla, L., Taylor, P.E., Rosenfield, R.E. & Stevens, C.E. (1998) Outcomes among 562 recipients of placental-blood transplants from unrelated donors. New England Journal of Medicine, 339, 1565-1577.
21. + bone marrow progenitor cells in vitro. Blood, 87, 1317-1325.
22. - cells and maintains progenitor cells in vitro. Blood, 87, 3563-3570.
23. Sui, X., Tsuji, K., Tanaka, R., Tajima, S., Muraoka, K., Ebihara, Y., Ikebuchi, K., Yasukawa, K., Taga, T., Kishimoto, T. & Nakahata, T. (1995) gp 1 30 and c-kit signalings synergize for ex vivo expansion of human primitive hemopoietic progenitor cells. Proceedings of the National Academy of Sciences of the United States of America, 92, 2859-2863.
24. Sutherland, H.J., Limsdorp, P.M., Henkelman, D.H., Eaves, A.C. & Eaves, C.J. (1990) Functional characterization of individual human hematopoietic stem cells cultured at limiting dilution on supportive marrow stromal layer. Proceedings of the National Academy of Sciences of the United States of America, 87, 3584-3589.
25. + cells. Journal of Experimental Medicine, 184, 1357-1364.
26. (+) human umbilical cord blood myeloid progenitors in serum-depleted culture medium. Annals of Hematology, 72, 131-135.
27. + cells an enhanced ex vivo expansion potential. Experimental Hematology, 22, 1264-1272.
28. Traycoff, C.M., Cornetta, K., Yoder, M.C., Davidson, A. & Srour, E.F. (1996) Ex vivo expansion of murine hematopoietic progenitor cells generate classes of expanded cells possessing different levels of bone marrow repopulating potential. Experimental Hematology, 24, 299-306.
29. Traycoff, C.M., Kosak, S.T., Grigsby, S. & Srour, E.F. (1995) Evaluation of ex vivo expansion potential of cord blood and bone marrow Hematopoietic progenitor cells using cell tracking and limiting dilution analysis. Blood, 85, 2059-2068.
30. Wagner, J.E., Kernan, N.A., Steinbuch, M., Broxmeyer, H.E. & Gluckman, E. (1995) Allogeneic sibling umbilical cord blood transplantation in children with malignant and non-malignant disease. Lancet, 346, 214-219.
31. Wagner, J.E., Rosenthal, J., Sweetman, R., Shu, X.O., Davies, S.M., Ramsay, N.K., McGlave, P.B., Sender, L. & Cairo, M.S. (1996) Successful transplantation of HLA-matched and HLA-mismatched umbilical cord blood from unrelated donors: analysis of engraftment and acute graft-versus-host disease. Blood, 88, 795-802.
32. Yonemura, Y., Ku, H., Lyman, S.D., & Ogawa, M. (1997) In vitro expansion of hematopoietic progenitors and maintenance of stem cells: comparison between FLT3/FLK-2 ligand and KIT ligand. Blood, 89, 1915-1921.
33. Young, J.C., Varma, A., di Giusto, D. & Backer, M.P. (1996) Retention of quiescent hematopoietic cells with high proliferative potential during ex vivo stem cell culture. Blood, 87, 545-556.
34. Zandstra, P.W., Conneally, E., Piret, J.M. & Eaves, C.J. (1998) Ontogeny-associated changes in the cytokine responses of primitive human haemopoietic cells. British Journal of Haematology, 101, 770-778.