A new modification of the 32P-post-labeling method to recover IQ-DNA adducts as mononucleotides

Mutagenesis

Volumn 14, Issue 2, 1999, Pages 239-242

  Ochiai, Masako ^a   Nakagama, Hitoshi ^a   Turesky, Robert J ^b   Sugimura, Takashi ^a   Nagao, Minako ^a  

^a NATIONAL CANCER CENTER RESEARCH INSTITUTE   (Japan)

^b NESTLÉ RESEARCH CENTER   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

2 AMINO 3 METHYLIMIDAZO[4,5 F]QUINOLINE; DEOXYGUANOSINE DERIVATIVE; DNA; HETEROCYCLIC AMINE; NUCLEASE; NUCLEOTIDE; PHOSPHODIESTERASE I; PHOSPHORUS 32; POLYNUCLEOTIDE 5' HYDROXYL KINASE; DNA (2 AMINO 3 METHYLIMIDAZO(4,5 F)QUINOLINE) ADDUCT; DNA-(2-AMINO-3-METHYLIMIDAZO(4,5-F)QUINOLINE) ADDUCT; QUINOLINE DERIVATIVE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; DIETARY INTAKE; DNA ADDUCT; DNA DETERMINATION; ENZYME ACTIVITY; GENOTOXICITY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; RAT; ANIMAL; CHEMICAL MODEL; FISCHER 344 RAT; LIVER; METABOLISM; METHODOLOGY; MUTAGEN TESTING;

ANIMALIA;

ANIMALS; CHROMATOGRAPHY; DNA ADDUCTS; LIVER; MODELS, CHEMICAL; MUTAGENICITY TESTS; QUINOLINES; RATS; RATS, INBRED F344;

EID: 0032587394     PISSN: 02678357     EISSN: None     Source Type: Journal    
DOI: 10.1093/mutage/14.2.239     Document Type: Article

References (13)

1. 32P-postlabeling assay. Carcinogenesis, 13, 1053-1074.
2. Cameron, V. and Uhlenbeck, O.C. (1977) 3′-Phosphatase activity in T4 polynucleotide kinase. Biochemistry. 16, 5120-5126.
3. 32P-postlabeling method. Jpn. J. Cancer Res., 85, 113-117.
4. 32P-postlabeling analysis of aromatic carcinogen:DNA adducts. Cancer Res., 45, 5656-5662.
5. 32P-postlabeling analysis of non-radioactive aromatic carcinogen-DNA adducts. Carcinogenesis, 3, 1081-1092.
6. Hall, M., She, M.N., Wild, D., Fasshauer, I. Hewer, A. and Phillips, D.H. (1990) Tissue distribution of DNA adducts in CDF1 mice fed 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and 2-amino-3,4-dimethylimidazo[4.5-f]quinoline (MeIQ). Carcinogenesis. 11, 1005-1011.
7. 32P-Postlabeling analysis of DNA adducts persisting for up to 42 weeks in the skin, epidermis and dermis of mice treated topically with 7,12-dimethylbenz[a]anthracene. Carcinogenesis, 6, 1117-1126.
8. 32P-postlabeling test for structurally diverse DNA adducts. Carcinogenesis, 7, 1543-1551.
9. Snyderwine, E.G., Roller, P.P. Adamson, R.H., Sato, S. and Thorgeirsson, S.S. (1988a) Reaction of N-hydroxylamine and N-acetoxy derivatives of 2-amino-3-methylimidazolo[4,5-f]quinoline with DNA. Synthesis and identification of N-(deoxyguanosin-8-yl)-IQ. Carcinogenesis, 9, 1061-1065.
10. 32P-postlabeling method to detect DNA adducts of 2-amino-3-methylimidazolo[4,5-f]quinoline (IQ) in monkeys fed IQ: identification of the N-(deoxyguanosin-8-yl)-IQ adduct. Carcinogenesis, 9, 1739-1743.
11. Tada, A., Ochiai, M., Wakabayashi, K., Nukaya, H., Sugimura, T. and Nagao, M. (1994) Identification of N-(deoxyguanosin-8-yl)-2-amino-3,4-dimethylimidazo[4,5-f]quinoline (dG-C8-MeIQ) as a major adduct formed by MeIQ with nucleotides in vitro with DNA in vivo. Carcinogenesis, 15, 1275-1278.
12. 2 atoms of guanine. Chem. Res. Toxicol., 7, 752-761.
13. Turesky, R.J. and Markovic, J. (1995) DNA adduct formation of the food carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) in liver, kidney and colo-rectum of rats. Carcinogenesis, 16, 2275-2279.