AlgT (σ22) controls alginate production and tolerance to environmental stress in Pseudomonas syringae

Journal of Bacteriology

Volumn 181, Issue 23, 1999, Pages 7176-7184

  Keith, Lisa M W ^a   Bender, Carol L ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALGINIC ACID; ALGU PROTEIN, PSEUDOMONAS AERUGINOSA; BACTERIAL PROTEIN; SIGMA FACTOR; TRANSCRIPTION FACTOR;

ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; ENVIRONMENTAL FACTOR; GENE EXPRESSION REGULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PSEUDOMONAS SYRINGAE; REGULATOR GENE; STRESS; TRANSCRIPTION REGULATION; AMINO ACID SEQUENCE; GENETIC COMPLEMENTATION; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MULTIGENE FAMILY; MUTATION; OXIDATIVE STRESS; PHENOTYPE; PSEUDOMONAS; SEQUENCE HOMOLOGY; TEMPERATURE; TIME; TRANSPOSON;

PSEUDOMONAS AERUGINOSA; PSEUDOMONAS SYRINGAE;

ALGINATES; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA TRANSPOSABLE ELEMENTS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC COMPLEMENTATION TEST; MOLECULAR SEQUENCE DATA; MULTIGENE FAMILY; MUTATION; OXIDATIVE STRESS; PHENOTYPE; PSEUDOMONAS; SEQUENCE HOMOLOGY, AMINO ACID; SIGMA FACTOR; TEMPERATURE; TIME FACTORS; TRANSCRIPTION FACTORS;

EID: 0032586070     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.23.7176-7184.1999     Document Type: Article

References (64)

1. Alvarez, M. E., R. I. Pennel, P. J. Meijer, A. Ishikawa, R. A. Dixon, and C. Lamb. 1998. Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell 92:773-784.
2. Barta, T. M., T. G. Kinscherf, and D. K. Willis. 1992. Regulation of tabtoxin production by the lemA gene in Pseudomonas syringae. J. Bacteriol. 174: 3021-3029.
3. Beattie, G. A., and S. E. Lindow. 1995. The secret life of foliar bacterial pathogens on leaves. Annu. Rev. Phytopathol. 33:145-172.
4. Bender, C. L., S. A. Young, and R. E. Mitchell. 1991. Conservation of plasmid DNA sequences in coronatine-producing pathovars of Pseudomonas syringae. Appl. Environ. Microbiol. 57:993-999.
5. Bolwell, G. P., and P. Wojtaszek. 1997. Mechanisms for the generation of reactive oxygen species in plant defence - a broad perspective. Physiol. Mol. Plant Pathol. 51:347-366.
6. Boucher, J. C., J. Martinez-Salazar, M. J. Schurr, M. H. Mudd, H. Yu, and V. Deretic. 1996. Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrA. J. Bacteriol. 178:511-523.
7. Boucher, J. C., M. J. Schurr, H. Yu, D. W. Rower, and V. Deretic. 1997. Pseudomonas aeruginosa in cystic fibrosis: role of mucC in the regulation of alginate production and stress sensitivity. Microbiology 143:3473-3480.
8. 2 in transgenic tobacco. Proc. Natl. Acad. Sci. USA 95:5818-5823.
9. Chitnis, C. E., and D. E. Ohman. 1993. Genetic analysis of the alginate biosynthetic gene cluster of Pseudomonas aeniginosa shows evidence of an operonic structure. Mol. Microbiol. 8:583-590.
10. Deretic, V., J. F. Gill, and A. M. Chakrabarty. 1987. Pseudomonas aeniginosa infection in cystic fibrosis: nucleotide sequence and transcriptional regulation of the algD gene. Nucleic Acids Res. 15:4567-4581.
11. Deretic, V., M. J. Schurr, J. C. Boucher, and D. W. Martin. 1994. Conversion of Pseudomonas aeruginosa to mucoidy in cystic fibrosis: environmental stress and regulation of bacterial virulence by alternative sigma factors. J. Bacteriol. 176:2773-2780.
12. DeVries, C. A., D. J. Hassett, J. L. Flynn, and D. E. Ohman. 1995. Genetic linkage in Pseudomonas aeruginosa of algT and nadB: mutation in nadB does not affect NAD biosynthesis or alginate production. Gene 156:63-67.
13. DeVries, C. A., and D. E. Ohman. 1994. Mucoid-to-nonmucoid conversion in alginate-producing Pseudomonas aeruginosa often results from spontaneous mutations in algT, encoding a putative alternative sigma factor, and shows evidence of autoregulation. J. Bacteriol. 176:6677-6687.
14. E subunit of Escherichia coli RNA polymerase: a second alternate σ factor involved in high-temperature gene expression. Genes Dev. 3:1462-1471.
15. Fakhr, M. K., A. Peñaloza-Vázquez, A. M. Chakrabarty, and C. L. Bender. 1999. Regulation of alginate biosynthesis in Pseudomonas syringae pv. syringae. J. Bacteriol. 181:3478-3485.
16. Farr, S., and T. Kogoma. 1991. Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiol. Rev. 55:561-585.
17. Figurski, D., and D. R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76:1648-1652.
18. Gacesa, P. 1998. Bacterial alginate biosynthesis - recent progress and future prospects. Microbiology 144:1133-1143.
19. Goldberg, J. B., W. L. German, J. L. Flynn, and D. E. Ohman. 1993. A mutation in algN permits trans activation of alginate production by algT in Pseudomonas species. J. Bacteriol. 175:1303-1308.
20. Govan, J. R. W., and V. Deretic. 1996. Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol. Rev. 60:539-574.
21. E). Proc. Natl. Acad. Sci. USA 92:7941-7945.
22. Jensen, E. T., A. Kharazmi, K. Lam, J. W. Costerton, and N. Høiby. 1990. Human polymorphonuclear leucocyte response to Pseudomonas aeruginosa grown in biofilms. Infect. Immun. 58:2382-2385.
23. Jones, J. D. G., and N. Gutterson. 1987. An efficient mobilizable cosmid vector, pRK7813, and its use in a rapid method for marker exchange in Pseudomonas fluorescens strain HV37a. Gene 61:299-306.
24. Kado, C. I., and S. T. Liu. 1981. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 145:1365-1373.
25. Kato, J., and A. M. Chakrabarty. 1991. Purification of the regulatory protein AlgR1 and its binding in the far upstream region of the algD promoter in P. aeruginosa. Proc. Natl. Acad. Sci. USA 88:1760-1764.
26. Keane, P. J., A. Kerr, and P. B. New. 1970. Crown gall of stone fruit. II. Identification and nomenclature of Agrobacterium isolates. Aust. J. Biol. Sci. 23:585-595.
27. Keen, N. T., S. Tamaki, D. Kobayashi, and D. Trollinger. 1988. Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 79:191-197.
28. Keith, L. M. W., J. E. Partridge, and C. L. Bender. 1999. dnaK and the heat stress response of Pseudomonas syringae pv. glycinea. Mol. Plant-Microbe Interact. 12:563-574.
29. Kidambi, S. P., G. W. Sundin, D. A. Palmer, A. M. Chakrabarty, and C. L. Bender. 1995. Copper as a signal for alginate synthesis in Pseudomonas syringae pv. syringae. Appl. Environ. Microbiol. 61:2172-2179.
30. King, E. O., M. K. Ward, and D. E. Raney. 1954. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44:2172-2179.
31. Kovach, M. E., R. W. Phillips, P. H. Elzer, R. M. Hoop III, and K. M. Peterson, 1994. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 16:800-802.
32. Lamb, C., and R. A. Dixon. 1997. The oxidative burst in plant disease resistance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48:251-275.
33. Lukomski, S., R. A. Hull, and S. I. Hull. 1996. Identification of the O antigen polymerase (rfc) gene in Escherichia coli O4 by insertional mutagenesis using a nonpolar chloramphenicol resistance cassette. J. Bacteriol. 178:240-247.
34. Mager, W. H., and A. J. J. Kruijff. 1995. Stress-induced transcriptional activation. Microbiol. Rev. 59:505-531.
35. Martin, D. W., M. J. Schurr, M. H. Mudd, and V. Deretic. 1993. Differentiation of Pseudomonas aeruginosa into the alginate-producing form: inactivation of mucB causes conversion to mucoidy. Mol. Microbiol. 9:497-506.
36. Martin, D. W., M. J. Schurr, M. H. Mudd, J. R. Govan, B. W. Holloway, and V. Deretic. 1993. Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients. Proc. Natl. Acad. Sci. USA 90: 8377-8381.
37. Martin, D. W., M. J. Schurr, H. Yu, and V. Deretic. 1994 Analysis of promoters controlled by the putative sigma factor AlgU regulating conversion to mucoidy in Pseudomonas aeruginosa: relationship to stress response. J. Bacteriol. 176:6688-6696.
38. Martínez-Salazar, J. M., S. Moreno, R. Nájera, J. C. Boucher, G. Espin, G. Soberon-Chávez, and V. Deretic. 1996. Characterization of the genes coding for the putative sigma factor AlgU and its regulators MucA, MucB, MucC, and MucD in Azotobacter vinelandii and evaluation of their roles in alginate biosynthesis. J. Bacteriol. 178:1800-1808.
39. 22 for expression of the alginate regulon in Pseudomonas aeruginosa and localization of its antagonist proteins MucA and MucB (AlgN). J. Bacteriol. 179:3711-3720.
40. May, T. B., and A. M. Chakrabarty. 1994. Isolation and assay of Pseudomonas aeruginosa alginate. Methods Enzymol. 235:295-304.
41. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
42. Mohr, C. D., J. H. J. Leveau, D. P. Krieg, N. S. Hibler, and V. Deretic. 1992. AlgR-binding sites within the algD promoter make up a set of inverted repeats separated by a large intervening segment of DNA. J. Bacteriol. 174: 6624-6633.
43. Murillo, J., H. Shen, D. Gerold, A. K. Sharma, D. A. Cooksey, and N. T. Keen. 1994. Characterization of pPT23B, the plasmid involved in syringolide production by Pseudomonas syringae pv. tomato. Plasmid 31:275-287.
44. Palmer, D. A., C. L. Bender, and S. B. Sharma. 1997. Use of Tn5-gusA5 to investigate environmental and nutritional effects on gene expression in the coronatine biosynthetic gene cluster of Pseudomonas syringae pv. glycinea. Can. J. Microbiol. 43:517-525.
45. Peñaloza-Vázquez, A., and C. L. Bender. 1998. Characterization of CorR, a transcriptional activator which is required for biosynthesis of the phytotoxin coronatine. J. Bacteriol. 180:6252-6259.
46. Peñaloza-Vázquez, A., S. P. Kidambi, A. M. Chakrabarty, and C. L. Bender. 1997. Characterization of the alginate biosynthetic gene cluster in Pseudomonas syringae pv. syringae. J. Bacteriol. 179:4464-4472.
47. Pier, G. B. 1998. Pseudomonas aeruginosa: a key problem in cystic fibrosis. ASM News 64:339-347.
48. Rehm, B. H. A., and S. Valla. 1997. Bacterial alginates: biosynthesis and applications. Appl. Microbiol. Biotechnol. 48:281-288.
49. Rich, J. J., and D. K. Willis. 1990. A single oligonucleotide can be used to rapidly isolate DNA sequences flanking a transposon Tn5 insertion by the polymerase chain reaction. Nucleic Acids Res. 18:6673-6676.
50. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
51. Schurr, M. J., and V. Deretic. 1997. Microbial pathogenesis in cystic fibrosis: co-ordinate regulation of heat-shock response and conversion to mucoidy in Pseudomonas aeruginosa. Mol. Microbiol. 24:411-420.
52. E) which controls mucoidy in cystic fibrosis isolates of Pseudomonas aeruginosa. J. Bacteriol. 177:5670-5679.
53. E-like family of stress sigma factors, by the negative regulators MucA and MucB and Pseudomonas aeruginosa conversion to mucoidy in cystic fibrosis. J. Bacteriol. 178:4997-5004.
54. Shankar, S., R. Ye, D. Schlictman, and A. M. Chakrabarty. 1995. Exopolysaccharide alginate synthesis in Pseudomonas aeruginosa: enzymology and regulation of gene expression. Adv. Enzymol. 70:221-255.
55. Simpson, J. A., S. E. Smith, and R. T. Dean. 1989. Scavenging by alginate of free radicals released by macrophages. Free Radical Biol. Med. 6:347-353.
56. Staskawicz, B. J., D. Dahlbeck, and N. T. Keen. 1984. Cloned avirulcnce gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max (L.) Merr. Proc. Natl. Acad. Sci. USA 81:6024-6028.
57. Sundin, G. W., and C. L. Bender. 1993. Ecological and genetic analysis of copper and streptomycin resistance in Pseudomonas syringae pv. syringae. Appl. Environ. Microbiol. 59:1018-1024.
58. Thurman, R. B., and C. P. Gerba. 1989. The molecular mechanisms of copper and silver ion disinfection of bacteria and viruses. Crit. Rev. Environ. Control 18:295-315.
59. 2 and NO: redox signals in disease resistance. Trends Plant Sci. 3:330-334.
60. Wozniak, D. J., and D. E. Ohman. 1994. Transcriptional analysis of the Pseudomonas aeruginosa genes algR, algB, and algD reveals a hierarchy of alginate gene expression which is modulated by algT. J. Bacteriol. 176: 6007-6014.
61. Xie, Z., C. D. Hershberger, S. Shankar, R. W. Ye, and A. M. Chakraharty. 1996. Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA. J. Bacteriol. 178:4990-4996.
62. Yu, J., A. Peñaloza-Vázquez, A. M. Chakrabarty, and C. L. Bender. 1999. Involvement of the exopolysaccharide alginate in the virulence and epiphytic fitness of Pseudomonas syringae pv. syringae. Mol. Microbiol. 33:712-720.
63. E and Pseudomonas aeruginosa AlgU: E. coli rpoE restores mucoidy and reduces sensitivity to reactive oxygen intermediates in algU mutants of P. aeruginosa. J. Bacteriol. 177:3259-3268.
64. Zielinski, N. A., R. Maharaj, S. Roychoudhury, C. E. Danganan, W. Hendrickson, and A. M. Chakrabarty. 1992. Alginate synthesis in Pseudomonas aeruginosa: environmental regulation of the algC promoter. J. Bacteriol. 174: 7680-7688.