Thioltransferase from Arabidopsis thaliana Seed: Purification to Homogeneity and Characterization

Molecules and Cells

Volumn 8, Issue 5, 1998, Pages 550-555

  Cho, Young Wook ^a   Kim, Joon Chul ^a   Jin, Chang Duck ^a   Han, Tae Jin ^b   Lim, Chang Jin ^a  

^a Kangwon National University   (South Korea)

^b Hallym University   (South Korea)

Author keywords

Arabidopsis thaliana; Glutaredoxin, Thioltransferase

Indexed keywords

CYSTEINE; DITHIOTHREITOL; GLUTATHIONE; MERCAPTOETHANOL; OXIDOREDUCTASE; PROTEIN DISULFIDE REDUCTASE (GLUTATHIONE); THIOL REAGENT; THIOLTRANSFERASE;

ARABIDOPSIS; ARTICLE; CHEMISTRY; DRUG EFFECT; ENZYME ACTIVATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; HEAT; ISOELECTRIC POINT; ISOLATION AND PURIFICATION; MOLECULAR WEIGHT; PH; PLANT SEED;

ARABIDOPSIS; CYSTEINE; DITHIOTHREITOL; ENZYME ACTIVATION; ENZYME STABILITY; GLUTATHIONE; HEAT; HYDROGEN-ION CONCENTRATION; ISOELECTRIC POINT; MERCAPTOETHANOL; MOLECULAR WEIGHT; OXIDOREDUCTASES; PROTEIN DISULFIDE REDUCTASE (GLUTATHIONE); SEEDS; SUBSTRATE SPECIFICITY; SULFHYDRYL REAGENTS;

EID: 0032585094     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Ahn, B.-Y. and Moss, B. (1992) Glutaredoxin homolog encoded by vaccinia virus is a virion-associated enzyme with thioltransferase and dehydroascorbate reductase activities. Proc. Natl. Acad. Sci. USA 89, 7060-7064.
2. Áslund, F., Ehn, B., Miranda-Vizuete, A., Pueyo, C., and Holmgren, A. (1994) Two additional glutaredoxins exist in Escherichia coli: glutaredoxin 3 is a hydrogen donor for ribonucleotide reductase in a thioredoxin/glutaredoxin 1 double mutant. Proc. Natl. Acad. Sci. USA 91, 9813-9817.
3. Axelsson, K. and Mannervik, B. (1983) An essential role of cytosolic thioltransferase in protection of pyruvate kinase from rabbit liver against oxidative inactivation. FEBS Lett. 152, 114-118.
4. Axelsson, K., Eriksson, S., and Mannervik, B. (1978) Purification and characterization of cytoplasmic thioltransferase (glutathione: disulfide oxidoreductase) from rat liver. Biochemistry 17, 2978-2984.
5. Bushweller, J.H., Áslund, F., Wüthrich, K., and Holmgren, A. (1992) Structural and functional characterization of the mutant Escherichia coli glutaredoxin and its mixed disulfide with glutathione. Biochemistry 31, 9288-9293.
6. Fernando, M., Sumimoto, H., Nanri, H., Kawabata, S., Iwanaga, S., Minakami, S., Fukumaki, Y., and Takeshuge, K. (1994) Cloning and sequencing of the cDNA encoding human glutaredoxin. Biochim. Biophys. Acta 1218, 229-231.
7. Flamgni, F., Marmiroli, S., Caldarera, C. M., and Guarnieri, C. (1989) Involvement of thioltransferase- and thioredoxin-dependent systems in the protection of 'essential' thiol groups of ornithine decarbozylase. Biochem. J. 259, 111-115.
8. Fuchs, J. A. (1977) Isolation of an Escherichia coli mutant deficient in thioredoxin reductase. J. Bacteriol. 129, 967-972.
9. Gan, Z.-R. and Wells, W. W. (1987) Preparation of homogeneous pig liver thioltransferase by a thiol:disulfide mediated pI shift. Anal. Biochem. 162, 265-273.
10. Gan, Z.-R. and Wells, W. W. (1986) Purification and properties of thioltransferase. J. Biol. Chem. 261, 996-1001.
11. Gan, Z.-R. and Wells, W. W. (1987) Identification and reactivity of the catalytic site of pig liver thioltransferase. J. Biol. Chem. 262, 6704-6707.
12. Gan, Z.-R., Polokoff, M. A., Jacobs, J. W., and Sardana, M. K. (1990) Complete amino acid sequence of yeast thioltransferase (glutaredoxin). Biochem. Biophys. Res. Commun. 168, 944-951.
13. Goswami, A. and Rosenberg, I. N. (1985) Purification and characterization of a cytosolic protein enhancing GSH-dependent microsomal iodothyronine 5′-monodeiodination. J. Biol. Chem. 260, 6012-6019.
14. Guagliardi, A., de Pascale, D., Cannio, R., Nobile, V., Bartolucci, S., and Rossi, M. (1995) The purification, cloning, and high level expression of a glutaredoxin-like protein from the hyperthermophilic archaeon Pvrococcus furiosus. J. Biol. Chem. 270, 5748-5755.
15. Hatekeyama, M., Tanimoto, Y., and Mizoguchi, T. (1984). Purification and some properties of bovine liver cytosol thioltransferase. J. Biochem. 95, 1811-1818.
16. Holmgren, A. (1976) Hydrogen donor system for Escherichia coli ribonucleoside-diphosphate reductase dependent upon glutathione. Proc. Natl. Acad. Sci. USA 73, 2275-2279.
17. Holmgren, A. (1979) Glutathione-dependent synthesis of deoxyribonucleotides. Purification and characterization of glutaredoxin from Escherichia coli. J. Biol. Chem. 254, 3664-3671.
18. Holmgren, A. (1989) Thioredoxin and glutaredoxin systems. J. Biol. Chem. 264, 13963-13966.
19. Höög, J. O., Jörnvall, H., Holmgren, A., Carlquist, M., and Persson, M. (1983) The primary structure of Escherichia coli glutaredoxin. Distant homology with thioredoxins in a superfamily of small proteins with a redox-active cystine disulfide/cysteine dithiol. Eur. J. Biochem. 136, 223-232.
20. Höög, J. O., Lindström, H., Jörnvall, H., and Holmgren, A. (1986) Cloning and expression of the glutaredoxin (grx) gene of Escherichia coli. Gene 43, 13-21.
21. Hopper, S., Johnson, R. S., Vath, J. E., and Biemann, K. (1989) Glutaredoxin from rabbit bone marrow. Purification, characterization, and amino acid sequence determined by tandem mass spectrometry. J. Biol. Chem. 264, 20438-20447.
22. Kim, H.-G., Park, E.-H., and Lim. C.-J. (1998) Thioltransferase from Schizosaccharomyces pombe: purification to homogeneity and some properties. Mol. Cells 8, 431-437.
23. Klintrot, I. M., Höög, J. O., Jörnvall, H., Holmgren, A. and Luthman, M. (1984) The primary structure of calf thymus glutaredoxin. Homology with the corresponding Escherichia coli protein but elongation at both ends and with an additional half-cystine/cysteine pair. Eur. J. Biochem. 144, 417-423.
24. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277, 680-685.
25. Lowry, O. H., Rogebrough, N. J., Farr, A. L., and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
26. Luthman, M. and Holmgren, A. (1982) Glutaredoxin from calf thymus: Purification to homogeneity. J. Biol. Chem. 257, 6686-6690.
27. Mannervik, B. and Axelsson, K. (1975) Reduction of disulphide bonds in proteins mixed disulphides catalysed by a thioltransferase in rat liver cytosol. Biochem. J. 149, 785-788.
28. Mannervik, B., Axelsson, K., Sundewall, A. C., and Holmgren, A. (1983) Relative contributions of thioltransferase- and thioredoxin-dependent systems in reduction of low molecular mass and protein disulphides. Biochem. J. 213, 519-523.
29. Mieyal, J. J., Starke, D. W., Gravina, S. A., Dothey, C., and Chung, J. S. (1991) Thioltransferase in human red blood cells: Purification and properties. Biochemistry 30, 6088-6097.
30. Minakuchi, K., Yabushita, T., Masumura, T., Ichihara, K., and Tanaka, K. (1994) Cloning and sequence analysis of a cDNA encoding rice glutaredoxin. FEBS Lett. 337, 157-160.
31. Morell, S., Follmann, H., and Häberlein, I. (1995) Identification and localization of the first glutaredoxin in leaves of a higher plant. FEBS Lett. 369, 149-152.
32. Padilla, C. A., Galisteo, E. M., Barcena, J. A., Spyrou, G., and Holmgren, A. (1995) Purification from placenta, amino acid sequence, structure comparisons and cDNA cloning of human glutaredoxin. Eur. J. Biochem. 227, 27-34.
33. Sa, J.-H., Kim, K., and Lim, C.-J. (1997) Purification and characterization of glutaredoxin from Cryptococcus neoformans. Mol. Cells 7, 655-660.
34. Sha, S., Minakuchi, K., Higaki, N., Sato, K., Kozo, O., Kurata, A., Yoshikawa, H., Kotaru, M., Masumura, T., Ichihara, K., and Tanaka, K. (1997a) Purification and characterization of glutaredoxin (thioltransferase) from rice (Oryza sativa L.). J. Biochem. 121, 842-848.
35. Sha, S., Yabushita, T., Minakuchi, K., Masumura, T., and Tanaka, K. (1997b) Structure of the rice glutaredoxin (thioltransferase) gene. Gene 188, 23-28.
36. Tsang, M. L.-S. (1981) Assimilatry sulfate reduction in Escherichia coli: Identification of the alternate cofactor for adenosine 3′-phosphate-5′-phosphosulfate reductase as glutaredoxin. J. Bacteriol. 146, 1059-1066.
37. Washburn, M. P. and Wells, W. W. (1997) Glutathione dependent reduction of alloxan to dialuric acid catalyzed by thioltransferase (glutaredoxin): a possible role for thioltransferase in alloxan toxicity. Free Radic. Biol. Med. 23, 563-570.
38. Wells, W. W., Xu, D. P., Yang, Y., and Rocque, P. A. (1990) Mammalian thioltransferase (glutaredoxin) and protein disulfide isomerase have dihydroascorbate reductase activity. J. Biol. Chem. 265, 15361-15364.
39. Wells, W. W., Yang, Y, Deits, T. L., and Gan, Z.-R. (1993) Thioltransferase. Adv. Enzvmol. Rel. Areas Mol. Biol. 66, 149-201.
40. Yang, Y., Gan, Z.-R., and Wells, W. W. (1989) Cloning and sequencing the cDNA encoding pig liver thioltransferase. Gene 83, 339-346.